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A unisexual reproductive structure of gymnospermous plants such as conifers and cycads, typically consisting of a central axis around which there are scaly, overlapping, spirally arranged sporophylls that develop pollen-bearing sacs or naked ovules or seeds.

球果单性裸子植物的生殖器，例如针叶树和铁树目植物，一般由覆满鳞片和褶皱的中心轴构成，长螺旋状排列的孢子叶，孢子叶最终成为花粉囊或裸露的孢子或种子

A unisexual reproductive structure of gymnospermous plants such as conifers and cycads, typically consisting of a central axis around which there are scaly, overlapping, spirally arranged sporophylls that develop pollen-bearing sacs or naked ''.

球果单性裸子植物的生殖器，例如针叶树和铁树目植物，一般由覆满鳞片和褶皱的中心轴构成，长螺旋状排列的孢子叶，孢子叶最终成为花粉囊或裸露的孢子或种子

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Objective To investigate the histomorphological changes of soft tissue after extrema expansion by overlapping tissue expansion.

目的：通过观察皮肤经重叠扩张至极限后的组织学变化。

By overlapping the charting of land desertification and the types of soil, the writer concludes that desertification is common to semi- hydromorphic soil and desert soil. And the reversion of alkaline soil shows the achievements of improvement of quality of alkaline soil.

将土地荒漠化图与土壤类型进行叠加，发现荒漠化在半水成土和漠土上发育比较普遍，而盐碱土类型上出现荒漠化逆转，说明多年来新疆对于盐碱地的改良和利用取得了有效的改善； 5。

The result showed that the imbricative cuticle scales arranged irregularly with different distance and irregular orientation. There were overlapping of cuticle cells, smoothing of the zigzag appearance of their edges, local denudation and attached debris.

观察结果表明：矿工头发超微结构的变化主要表现为毛小皮鳞片排列疏密不均，毛小皮间的间距有宽有窄、走向不甚规则；观察到有数个毛小皮细胞相互重叠，游离缘锯齿状变得较为平滑，局部出现片层脱落及在毛干表面附着一些碎片。

They interweave together each other, tend to changing constantly, power and shape , they fine long hair sometimes flow , sometimes congeal and stop , erupt sometimes irregular, disappear sometimes," some such things change like lights of forest like that in feeling of us, it hand in again and the overlapping:" Gather into certain form again when they have not offsetted and concealed each other, but this kind of form in often resolving, or break out in the fierce conflict for the passion, or become changed beyond recognition in this kind of conflict , all these things blend subjective reality make up man we call that inalienably for an organic whole " The inherent life " Thing promptly emotions human so-called.

它们相互交织在一起，不断地改变着趋向，强弱和形态，它们毫无规律地时而流动，时而凝止，时而爆发，时而消失，&这样一些东西在我们的感受中就象森林中的灯火那样地变化不定，相互交又和重迭&当它们没有相互抵消和掩盖时便又聚成一定的形状，但这种形状又在时时的分解着，或是在激烈的冲突中爆发为激情，或是在这种冲突中变得面目全非，所有这些交融为一体不可分割地主观现实就组成丁我们称之为&内在生命&的东西即所谓人类情感。

The optimized classification andtracking results are determined by the viewpoint correspondence and data fusionamong the overlapping cameras.By use of the different view directions of theoverlapping cameras,object classification accuracy and tracking stabilization incomplex occlusion scenes are improved.

借助于重叠摄像机之间不同的视角方向，利用摄像机之间的视点对应和数据融合决定最优的分类与跟踪结果，提高了遮挡状态下运动目标分类的准确度和目标跟踪的稳定性。

Inductiveness the above points, special gravure packaging software: cyclophos phamide connectivity software; II pages joint image stitching software; and aim at a spot-facture feature software about trapping features software (to compensate for business card printing and membership card making alignment deviation, the various color version minor overlapping contours, mainly targets of plastic as gravure printing), etc.

归纳以上各点，包装凹印制版专用的软件有：①连接功能软件；②制作无接缝图像的拼接功能软件；③专色制作分色功能软件；④陷印功能软件（为弥补制卡和会员卡制作套准偏差，将各色版图像轮廓进行少量重叠，主要针对塑膜凹印）等。

While on one hand the two are correlative, overlapping and interactive, they are on the other hand independent and interfluvial

因此，确立现代大传播观念，将新闻传播纳入大众传播和整个社会信息与情报传播的宏大范围内，是当务之急。

There had been a moment during the breakfast meeting, though, after the backslapping and the small talk and when all of us were seated, with Vice President Cheney eating his eggs Benedict impassively and Karl Rove at the far end of the table discreetly checking his BlackBerry, that I witnessed a different side of the man.

那次早餐会期间，在表示过关心和寒暄以后，我们所有人坐了下来，我注意观察了一下，副总统切尼面无表情地吃着班尼迪克蛋（Eggs benedict，源自美国，以英式松饼、火腿、水煮蛋以及荷兰酱组合而成），卡尔。罗夫在餐桌的远端谨慎地经常查看他的黑莓手机，我目睹了他所不为人知的一面。

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