查询词典 nucleases

The group of low migration nucleases were prepared by ammonium carbonate processing +acid denaturation + thermal denaturation + acetone extraction.

在提取过程中产生两组核酸酶，在SDS-PAGE-DNA功能胶中迁移率较慢的一组采用碳酸铵处理+酸变性+热变性+丙酮沉淀的提取方法来获得较好的层析样品。

The importance of such proteins in the organised processing of pro-proteins such as nucleases (Nicieza et al. 1999) and the dismantling of cell structure, rather than uncoordinated autolysis (Miguelez et al. 1999), makes an attractive hypothesis for studying development of bacteria and the evolutionary important processes of cell death.

在亲蛋白质等组织处理的重要性，如核酸蛋白（尼谢萨等。1999年）和细胞结构，而不是不协调的自溶（Miguelez等人，拆除。1999年），为使研究开发的一个有吸引力的假说细菌和细胞死亡进化的重要过程。

This was circumvented using specially engineered 'zinc-finger' nucleases as molecular scissors to cut DNA inside cells at a specific sequence.

现在，用特殊设计的"锌手指"核酸酶作为分子剪刀来在细胞内的特定序列上切割DNA，便能绕开这个问题。

We have been interested in design and synthesis of macrocyclic lanthanide complexes which are capable of catalytically hydrolyzing phosphodiester bonds. Because of their high thermodynamics stability, low kinetics lability, high coordination numbers, and charge density, macrocyclic lanthanide complexes will be suitable as artificial nucleases.

合成设计能促进磷酸酯键水解的金属错合物，一直是我们实验室很感兴趣的研究领域，尤其是以镧系金属离子大环配位子错合物这方面的研究，因其具有高度的热力学稳定、动力学稳定、高配位数及电子密度等特性，使得镧系金属大环错合物非常适合作为人造水解酶。

Naturally occurring oligonucleotides are susceptive to degradation by nucleases in blood and cells.

由于反义寡核苷酸是大分子物质，细胞膜对其通透性低，因此必须在载体的帮助下，才能有效进入细胞。

PNAs are uncharged DNA mimics, which are resistant to nucleases and peptidases, and hybridize with complementary DNA and RNA with high affinity.

PNA是一类不带电荷的DNA相似物，不受核酸酶和肽酶的作用，可以和cDNA、RNA高亲和性杂交。

Positive rate was relatively high, and the foreign gene could also maintain in testicular tissue for a long time, it could still be detected in the tissue in 7 months after being injected. However. Deferent results could be gained when the detection were made with different primers on different sites of the recombinant plasmid. This could be explained by transgene lost when the foreign gene recombinated and integrated in heterogenetic cell, or transgene degradated by cell nucleases.

然而，我们也看到，在后代检测中，利用不同的引物扩增重组质粒不同部位时，检测结果存在差异，转基因阳性率不同，这种差异说明当外源基因进入到异源细胞内，在重组整合时发生了外源基因丢失的现象，并由此导致外源基因的整合率下降，也可能是受细胞内核酸酶的作用，使得外源基因遭到降解。

Exploring the Structures, Coding Genes and Characteristics of Lumbricus Bimastus Nucleases EWD_1,2,3

双胸蚓组织中核酸酶EWD_（1，2，3）的结构、基因和相关性质研究在线阅读整本下载分章下载分页下载

Results1. Preparation of rude extracts of Lumbricus Bimastus nucleases and its purification Two groups of nucleases with different characters lied in the DNA-casting SDS-PAGE.

结果1。双胸蚓组织中核酸酶粗提物的制备及核酸酶的纯化应用本室建立的SDS-PAGE-DNA功能胶测活方法对核酸酶提取过程进行监测。

The research on artificial nucleases has been one of the most active frontiers of biochemistry and molecular biology. The investigation on DNA and transition metal complexes as the potential artificial nucleases is the significant branch of bioinorganic chemistry, and is of important theory and application values.

人工核酸切割试剂的研究一直是生物化学和分子生物学最为活跃的研究领域之一，过渡金属配合物作为潜在的人工核酸酶与DNA的作用是生物无机化学研究领域的重要分支，具有很重要的理论价值和应用价值。

It goes back to what I told you...

现在回到我告诉过你的。。。

With a supporter in Mr Charest and an admirer in Mr Dumont, Mr Harper may be encouraged to call an election himself.

由于在沙雷那边有个支持者，杜蒙那边有个崇拜者，鼓励哈珀为自己举行一次选举。