查询词典 northern

The cloning, sequencing, homological blasting and Northern blotting results of 5 desiccation-induced cDNAs and 3 phosphate induced cDNAs implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as G protein, protein kinase, VP3and MAD3-like genes might be involved in dehydration in the resurrection plant Boea hygrometrica.

对其中5个脱水特异诱导表达的cDNA（包括可能与复苏能力有关的cDNA）和3个磷酸盐处理诱导表达的cDNA进行克隆测序、同源性探测和Northern杂交检测表明，牛耳草脱水过程中诱导表达的基因可能涉及到脱水胁迫的信号转导、调节基因的级联作用（VP3，MAD3样基因等）、结构基因产物调节细胞结构在脱水胁迫中的稳定性等。

Among these techniques, Northern blot, ribonuclease protection assay, real-time quantitative polymerase chain reaction, which are based on differentially screening and hybridization technology, have been applied most frequently.

本文概述了用于检测mRNA丰度的常规分析方法，即Northern印迹、RNase保护试验和实时定量PCR技术，特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。

Secondly, Northern blot showed that OsBP-73 was weakly expressed in root, leaf and immature seed.

Northern印迹结果表明OsBP-73基因在水稻根，叶片和未成熟种子中均有较低水平的表达，而且在各组织中的表达没有明显的差异。

Eleven differentially exp ressed fragmentswere confirmed by bulked segregant analysis and reverse Northern blotting, further cloned and sequenced.

经过BSA 分析和反向Northern杂交鉴定，获得了11个差异表达的cDNA片段，并进行了克隆、测序和序列同源性检索分析。

Methods: The traditional method of chronic serum sickness was modified by adding followings: one kidney removed, intraperitoneal inject LPS etc., and the effect of Shenhua Tablet on 24h proteinuria, blood biochemical indices, kidney function, pathology and immunofluorescence changes was observed compared with Fosinopril, and and the effect of Shenhua Tablet on the ED-1 positive cell number in the glomerulus, protein and mRNA expression and activation of Stat3 and protein and mRNA expression of TIMP-1 in the kidney tissue, and a-SMA positive mesangial cells were observed compared with Fosinopril using western blot, northern blot and immunohistochemistry methods.

对传统的大鼠慢性血清病肾炎模型制作方法进行以下改良：切除大鼠一侧肾脏、腹腔注射大肠杆菌内毒素、以及改尾静脉注射为腹腔注射。与西药福辛普利作对照，观察中药肾华对改良慢性血清病肾炎大鼠尿蛋白、血生化、肾功能、肾脏病理变化的影响。并通过免疫组化、Western蛋白印迹及Northern杂交方法，观察中药肾华对改良慢性血清病肾炎大鼠肾组织肾小球内炎细胞浸润、Stat3蛋白与基因表达及活化、以及其下游调控的TIMP-1蛋白与基因表达、系膜细胞增殖的影响。

Transferred the NP-1 gene into C. ellipsoidea by electroporation with high efficiency expression vector pBinUΩNP1. PCR, Southern blot and Northern dot blot analysis of the G418resistant alga strains indicated the stable integration and correct transcription of NP-1 in C. ellipsoidea genome. In vitro antagonistic assays showed the proper expression of defense gene in C. ellipsoidea. Transgenic C. ellipsoidea is antagonistic to gram negative bacteria—Bacillus subtonic, gram positive bacteria—Escherichiacoli and fungi—Fusarium oxysporum to some extent.

以椭圆小球藻完整细胞为受体，采用电激转化法将连接在高效表达载体pBinUΩNP1上的兔防御素NP-1基因导入小球藻，通过PCR、Southern杂交、Northern点杂交对转基因小球藻进行了分子检测，同时通过体外离体抑菌实验检测了兔防御素NP-1基因表达产物的活性，结果表明NP-1基因已稳定整合到小球藻基因组中，并进行了正确转录和表达。

Total RNA samples used in Northern blotting were extracted from the light-exposed and light-interrupted cortex tissue of tuberous root of the two turnip cultivars.

Northern分析采用的RNA样品，是从上述两品种的光处理和暗处理的肉质根部周皮层中提取的总RNA。

Northern blot and dot blot hybridizations indicate that it is expressed ubiquitously in human 50 tissues and 7 cancer cell lines.

Northern杂交和点杂交分析显示，该基因以大约5.4kb的单一转录物广泛表达于人体各种组织，而且在人部分肿瘤细胞中高表达。

An article from Psittacine Vol.52 (Aug.2002) describes how this can be done. There is an Indian Tribes, Zuni, in Northern American that use different feathers for different ceremonies at various times of the year.

在PsittaSceneVol.52 (Aug 2002)内有一篇文章提到，美国有一个部族(Zuni， a Northern American Indian tribe)在举行不同议式的时候，都会用到大量雀鸟的羽毛。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。