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Methods Rabbits were randomized to three groups. Complex model group were fed with feed containing 0.5% cholesterol for 3 weeks. At the fourth, eighth and twelfth weeks, rabbits were given injection of lipopolysaccharide (1 μg) at auricular artery, cervical part and fold inguen, respectively. Normal group were fed with normal diet, while High cholesterol group were fed with feed confaining 0.5% cholesterol only. After 16 weeks, vascular tissues were analyzed using morphometric and histological methods, and the sera were checked for cholesterol, LDL-C, C-reaction protein and tumor necrosis factor-α. Results The cholesterol and LDL-C of High cholesterol group were increased, compared to those of normal group.

给含0.5%胆固醇的饲料,3周后,分别在第4、8、12周采用耳动脉内、颈部、腹股沟处肌肉注射大肠埃希菌脂多糖,并设立正常组和单纯高脂组。16周后观察兔的一般状态,取血清检查血脂六项、C-反应蛋白和TNF-α,取耳动脉、颈动脉、主动脉弓、胸主动脉、腹主动脉、髂动脉、肝脏,放置4%多聚甲醛中过夜,常规行HE染色,检查血管病变和相关脏器病变情况。

Based on this,We designed a series of experiments to study the treatment and its mechanism of Iridoids composition of Valeriana jatamansi Jones of the IBS animal model.1 Research on Iridoids composition of Valeriana jatamansi Jones in the role of gastrointestinal function.1.1 Effection of Iridoids composition of Valeriana jatamansi Jones on gastrointestinal function of normal miceMethods:Compared with normal group and YJBH group,mice which has given Iridoids composition of Valeriana jatamansi Jones as 71.2mg/kg,35.6mg/kg,17.8mg/kg,8.9mg/kg was detected gastric emptying and small intestinal propulsion after 4 days drug given.Results:Compared with normal group,gastric emptying and small intestinal propulsion in 35.6mg/kg and 17.8mg/kg group was inhibited.

课题通过观察蜘蛛香环烯醚萜对正常及IBS模型动物的胃肠道功能、精神活动的影响,以及蜘蛛香环烯醚萜干预下IBS大鼠结肠、血清、中枢5-HT含量,结肠5-HTR3A受体的mRNA和色氨酸羟化酶(TPH1)的mRNA表达,结肠和血浆中SP和VIP含量以及结肠中肥大细胞分布,探讨蜘蛛香环烯醚萜对腹泻型IBS的治疗作用及其作用机理,为临床治疗IBS提供实验依据。1蜘蛛香环烯醚萜对胃肠功能的作用目的:探讨蜘蛛香环烯醚萜对胃肠功能的影响1.1蜘蛛香环烯醚萜对正常小鼠胃肠功能的作用方法:正常小鼠灌胃给予蜘蛛香环烯醚萜71.2mg/kg、35.6mg/kg、17.8mg/kg、8.9mg/kg与空白组和越鞠保和丸组对照,给药4日后测定动物的胃排空率和小肠推进率。

Then the index of ulcer was measured and the ulcer inhibitory rate was measured.Results: The weight of ear of mice treated with XSG was reduced compared with that of normal group,and the joint swelling of rat was less than that of normal group. The latence of sole-ache caused by hot-plate and bellyache caused by acetic were both delayed and the times of skewing body within 15 minute were reduced compared with that of the normal group.

结果:香砂六君颗粒对小鼠二甲苯性耳廓肿有明显对抗作用;对大鼠蛋清性关节肿有一定对抗作用;对热板和醋酸所致小鼠疼痛香砂六君颗粒能明显延长其舔足潜伏期和减少扭体次数;对大鼠利血平型及乙酸型胃溃疡香砂六君颗粒组的溃疡指数从模型组的46.26±1.64mm2和23.02±2.05mm2减少为34.75±0.82mm2和13.84±1.2mm2;对大鼠利血平型及乙酸型胃溃疡香砂六君颗粒的溃疡抑制率分别为51.43%和39.87%。

WTHZ Results The telomerase activity and expression of telomerase rela ted genes were significantly higher in 42 leukemia patients in progress stage th an that in complete remission/chronic stage and in normal/anemia cases (P.01).The telomerase activity and expression of TP1,hTR gene in 14 cases of acute leuk emia complete remission/chronic myelogenous-chronic stage were h igher than that in normal/anemia cases (P.05). Low levels of telom erase activi ty and telomerase related genes expression could be detected in part of normal b one marrow cells. The levels of telomerase activity and telomerase related genes expression in 3 cases of anemia increased after short-term culture.

结果 42例白血病进展阶段标本的端粒酶活性和相关基因表达水平高于急性白血病完全缓解期、慢性粒细胞白血病慢性期、正常和贫血标本(P.01);14例急性白血病完全缓解期和慢粒慢性期标本端粒酶活性和TP1、hTR基因表达水平高于正常和贫血标本(P.05);在部分正常和贫血标本中检测到相对低水平的端粒酶活性和相关基因表达,3例贫血标本经短期常规培养后检测到端粒酶活性和基因表达增高。

Bamboo first crystallizes then lignifies during bamboo shoot growth and basically forms crystal structure in one month. 1-year-old bamboo culm ends growth and its height,thickness, volume almost change little, but culm tissue is small and tender and there is more water and less substance in it. Bamboo crystal proportion keeps invariable during this period.2-year-old bamboo culm begin to lignify,its cellulose proportion debases and its crystal proportion decreases. Bamboo lignin debases, crystal proportion changes a little in the end of this period.Many-old-year bamboo culm crystal proportion is almost invariable. Freaky bamboo cell wall crystal region's thickness is less than normal bamboo's. Bamboo cross section crystal area and the ratio of long axis to short axis are less than normal bamboo's,and roundness,irregular index, the fiber cap density per unit area increase more than normal bamboo's.

竹笋生长阶段是先结晶后木素化的过程,一个月内细胞壁结晶组织基本形成;2)一年生竹材的植株秆茎生长结束,秆茎的高度、粗度和体积变化不大,但秆茎的组织幼嫩,含水量高,干物质少,竹材结晶度基本不变;3)二年生竹材的植株开始木质化,纤维素比例降低,结晶度减少,到后期竹子木质化降低,竹材结晶度变化不大;4)多年生竹材的结晶度没有太大的变化,结晶度大小处于波动状态;5)畸形竹材纤维帽细胞壁结晶区的厚度比正常竹小,其横截面结晶区的面积、纤维帽长轴与短轴比有所减少,而圆形度、异型指数有所加大,单位面积纤维帽的密度增大。

Thirty two point six percent (42/129) of menopausetransition women and 18.9%(60/318) of menopause women had breast structure changes,〓=9.26, P<0.01. The frequency of breast structure changes decreased with decreasing ofserum of 〓 level and increasing of age. Four (4/29, 13.8%) in HRT group had breaststructure changes, which disappeared after discontinuing HRT or reducing the dose of HRTin 3 subjects. Conclusions: Most of normal menstrual women and menopause transitionwomen experienced Mastalgia; Mastalgia occurred in 89.7% of subjects and lasted forabout 2 M. in the 1-year ccHRT; Image of breast ultrasound changed following menstrualcycle in normal menstrual women; Breast pain and breast structure change may be causedby higher 〓 and P level in luteal phase; The breast structure changes of HRT user weresimilar to that in normal women and were reversible.

大部分正常月经妇女和绝经过渡期妇女有周期性乳房胀疼,绝经后妇女应用17 β-雌二醇2mg加醋酸炔诺酮1mg连续联合治疗1年中大部分使用者有持续约两个月的乳房胀疼;乳腺体层厚度随体内雌孕激素水平的下降和年龄的增加而逐渐萎缩,绝经后妇女应用17 β-雌二醇2mg加醋酸炔诺酮1mg连续联合治疗1年后,对乳腺体层的厚度没有明显的影响;超声下乳腺结构改变主要受体内雌孕激素水平的影响,正常月经妇女中1/3的乳腺结构改变具有可逆性,绝经过渡期和绝经后妇女中有一半的乳腺结构改变的特征类似正常月经妇女具有可逆性的乳腺结构改变;绝经后妇女应用17 β-雌二醇2mg加醋酸炔诺酮1mg连续联合治疗1年后,少数使用者出现的乳腺结构改变可随药物剂量的调整或停药而恢复;对长期HRT使用者仍需加强乳腺的监测,长期HRT对乳腺的影响值得进一步研究。

Monocellular Raman spectrums of normal liver cell line (LO2) and carcinoma liver cell line (SMMC-7721) were studied with laser tweezers Raman spectrums system and each cell was investigated in three different points, the result of which showed; significant differences of average Raman spectrums were found between the normal cell and the carcinoma one; And, the spectra line of carcinoma cell became weak at the whole; Additionally, the spectral peak intensity ratio of normal cell at 1658cm^(-1) and 1450cm^(-1) was 0.63 while that of carcinoma was 0.99; Moreover, the structure and the amount of protein; nucleic acid, lipids and other such molecules had also changed.

本工作利用激光镊子拉曼光谱系统研究了人正常肝细胞株(LO2)和肝癌细胞株(SMMC-7721)的单细胞拉曼光谱,对于每个细胞在不同部位测三个点。实验结果显示:正常细胞和癌细胞的平均拉曼光谱存在显著差异;癌细胞谱线强度整体变弱;正常细胞的1658cm^(-1)处峰和1450cm^(-1)处峰的强度比值为0.63,癌细胞的为0.99;癌细胞的核酸、蛋白质、脂类等重要生物分子在结构或含量上都发生了不同的改变。

Each female child has the following chances of inheriting from her mother: a 50% risk of inheriting one faulty X gene and thus being a normal carrier and a 50% chance of inheriting the normal X gene and thus being a normal noncarrier.

各个女性孩子有继承的以下机会从她的母亲: 50%风险继承一个有毛病的 X 基因和如此是一个正常载体和50%机会的继承正常 X 基因和如此是法线 no carrier 。

Two hundred 16-day-old of age commercial broiler chickens were randomly divided into 4 groups as follows: 50 birds were exposed to normal temperature (20℃) and peritoneally injected with normal saline as control group; 50 birds were exposed to low temperature (7-9℃) and peritoneally injected normal saline as low temperature group; 50 birds were exposed to low temperature and peritoneally injected with low dose BQ123 as low temperature low dose BQ123 group; 50 birds were exposed to low temperature and peritoneally injected high dose BQ123 as low temperature high dose BQ123 group.

本试验采用高选择性ETA受体拮抗剂BQ123预处理低温肉鸡,探讨ET在低温诱发AS模型的肺血管重塑和肺动脉高压发生发展中的作用。200只16日龄AA肉鸡随机均分为4组:常温(20℃)对照组、低温(7~9℃)对照组、低温低剂量BQ123组和低温高剂量BQ123组。23日龄、30日龄时进行肺动脉压的测定,肺组织取材、石蜡切片,Weigert间苯二酚复红染色。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色:翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成;基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质,在翼状胬肉体部深层基质中存在粗糙的纤维组织;正常球结膜组织细胞排列整齐;基质为疏松结缔组织,胶原纤维平行排列,其间可见成纤维细胞,散在少量中性粒细胞、毛细血管;Masson染色:翼状胬肉浅层基质中存在致密的胶原纤维染色,深层基质中的胶原纤维存在变性样改变;VG染色:翼状胬肉组织深层基质中存在大量变性的胶原纤维,其间夹杂黑色的弹性纤维;免疫组化染色法:MMP-3在翼状胬肉上皮细胞中呈强表达,成纤维细胞中呈中等强度表达,血管内皮细胞中未见表达;LN在血管壁中呈强表达,在上皮细胞基底膜中呈中等强度表达,在整个基质中未见明显表达;col Ⅲ在整个翼状胬肉基质中呈强表达。

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Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化,采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。

The latest results are published online January 13 in the Journal of the National Cancer Institute. They come from a case–control study that involved 459 cases,"which, for the rarity of this cancer, is a very big study," Dr. Stang said.

Stang医师表示,这项最新的研究结果线上发表在1月13日的国家癌症机构期刊上,研究来自一项收纳459个病例的病例控制研究,这是一项非常大的研究。