查询词典 mutation stop

YMDD mutation: HBV YMDD mutation were developed in 12 lamivudine recipients, whereas the treated group had no incidence of HBV YMDD variation, there were significant difference between two groups (p=0.000). YMDD mutation resulted in increase of the mean HBVDNA levels (p=0.000), but no contribution to serum ALT diversification (p＞0.05). 2. Precore nt1896 mutation: Precore nt1896 mutation incidence had no difference between lamivudine recipients and objects who received combination therapy of Kidney-invigorating Granule and Marine Injection, and patients of HBeAg negative and HBeAg positive had a close incidence of precore mutation as well as. Precore nt1896 mutation had no influence on serum ALT level. The occurrence of precore nt1896 mutation contributed to serum HBVDNA load increased and worse response of HBVDNA extenuation. 3. HBV genotype: The baseline of serum HBVDNA gradually increase in turn of genotype B, genotype C and genotype B/C. Genotype B seemed to obtain higher rate of serum HBVDNA loss and HBeAg/anti-HBe seroconversion after combination therapy of Kidney-invigorating Granule and Marine Injection, differencing with lamivudine therapy.

疗效相关因素研究：1YMDD变异：对照组有12例检出YMDD变异，治疗组无1例检出，两组比较有显著性差异（P=0.000）；发生YMDD变异者血清HBVDNA和ALT水平均有所升高，HBVDNA平均水平高于非变异者（P=0.000）；血清ALT与非变异者无明显差异（P＞0.05）。2前C区nt1896变异：前C区nt1896变异发生率在治疗组和对照组以及HBeAg阴性和HBeAg阳性患者之间均无明显差异（P＞0.05）；1896c变异对血清ALT水平无明显影响；治疗组发生1896c变异者，其HBVDNA平均水平较之未变异者明显升高（P＜0.05），治疗后HBVDNA下降幅度明显低于未发生1896c变异者（P=0.000）。3HBV基因型：血清HBVDNA基础水平在B、C、B/C混合型间存在明显差异（P＜0.05），按B型、C型、B/C混合型依次升高；治疗组HBVDNA阴转率、HBeAg/抗-HBe血清转换率，B型似乎高于C型和B/C混合型，但无统计学意义（P＞0.05）；对照组HBVDNA阴转、HBeAg/抗-HBe改变在B、C、B/C混合型间无明显差异（P＞0.05）。

The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.

结果显示：先证者尿检呈强阳性；其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变，为半合子突变，而其母、其姐为杂合突变；正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变；不同物种氨基酸序列的同源性比对显示： c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守；酶活性测定的结果显示：先证者的IDS酶活性仅为2.3 nmol/4 h/mL，大大低于正常值，而其父的为641.9 nmol/4 h/mL，其母的血浆酶活性为95.8 nmol/ 4h/mL，其姐的为103.2 nmol/4 h/mL。

Studies on the mutant breeding of high-yield gibberellins strain The protoplast of Gibberella fujikuroi m978 were treated by mutation breeding forhigh-yield gibberellins, LiCl mutation was better than DES mutation or UV irradiation ifsingle mutation, but any single mutation was worse than compound mutation by whichthe mutants were more overproducting and genetically stable.

采用传统诱变育种，筛选高产菌株在原生质体条件下，对赤霉素产生菌m978的原生质体进行菌种选育；使用单因子诱变方法，LiCl处理的效果优于DES诱变和UV诱变；复合诱变比单因子诱变更容易得到高产突变株，且获得的高产突变株稳定性更优。

I'll never stop I don't know, will you believe me After all is said and done All the lies, how I regret them baby Now, I am the loser And you're shining like the sun Tell me why can't I still be the one, alright I will never stop Until you're mine I can wait forever, till the end of time Cause my heart is in your hands Don't you understand I'll never stop I'll never stop How could I ever When my heart is in your hands And I know, baby there is no turning back They say that I'm crazy And I kind of understand How I wish for this nightmare to end Oh yes I will never stop Until you're mine I can wait forever, till the end of time Cause my heart is in your hands Don't you understand I'll never stop I'll never stop Ooh ooh, ooh ooh, ooh oohooh Do you believe me, when my heart is in your hands Don't you understand I'll never stop I will never stop Till the end of time Heart is in your hands I'll never stop Until you're mine I can wait forever, till the end of time Cause my heart is in your hands Don't you understand I'll never stop ..

我将从不停下来我不知道，你将相信我终归被说和做所有谎话，如何我惋惜他们婴儿现在，我是输者同时，你发光像太阳一样告诉我为什么我仍然不能是一，alright 我将从不停下来直到你是我的我永远能等待，直到时间的末端原因我的心在你的手中你不要理解我将从不停下来我将从不停下来如何能我曾经当我的心在你的手中时同时，我知道，婴儿没有往回旋转他们说我是疯狂的同时，我理解的种类我如何希望这恶梦结束啊是我将从不停下来直到你是我的我永远能等待，直到时间的末端原因我的心在你的手中你不要理解我将从不停下来我将从不停下来 Ooh ooh，ooh ooh，ooh oohooh 做当我的心在你的手中时，你相信我，你不要理解我将从不停下来我将从不停下来直到时间的末端我将从不停下来直到你是我的我永远能等待，直到时间的末端原因我的心在你的手中你不要理解我将从不停下来。。

Results The p53 gene mutation rate of colorectal cancer was 50%. The rate of apoptosis of tumor cells having mutation of p53 gene (22.11%) was much lower than that having no p53 mutation (40.57%, P＜0.05). Heteroploid cells had been found in 10/13 tumor samples with p53 gene mutation and 6/13 samples without p53 mutation (P＜0.05). The rate of apoptosis had no difference between the heteroploid cases and diploid cases.

结果 大肠癌标本p53基因突变率为50%；其中突变组肿瘤细胞凋亡率(22.11%)明显低于未突变组细胞凋亡率(40.57%)，(P＜0.05)；突变组异倍体细胞百分率(占76.9%)明显高于未突变组(占46.2%)，(P＜0.05)；异倍体肿瘤细胞凋亡率为34.0%，二倍体肿瘤细胞凋亡率为40.7%，二者差异无显著性(P＞0.05)。

I can't stop my love for you 2つの爱が离れてしまわないように 1つに抱いててそれでも不安な夜は访れるものだからねそんなときはギュッと大げさに抱きしめていてかけがえのないもの君がたくさんここにくれただけど何よりもいちばんかけがえのないものは君なんだ I can't stop my love for you 2人语った未来に君との居场所があるように Oh baby I can't stop my love for you 君のすべてになってねつないだ手信じて歩いてゆくと誓うよ发音: I can't stop my love for you futari katatta mirai ni kimi to no ibasho ga aru you ni Oh baby I can't stop my love for you kimi no subete ni natte ne tsunaida te shinjite aruite yuku to chikau yo futari no me no mae nazomeki hirogatta mikansei na chizu demo totemo totemo kokoro ugoita… donna kotoba narabete mite mo katarikirenai omoi kono ai ga sasayaku yo itoshisa no imi o I can't stop my love for you futari ayumu supiido wa kitto onaji hazu ja nai kara ne moshi mo kimi no tonari kono te ga hanareta toki wa miushinawanai you ni kitto mukae ni kite ne "daikirai" nante uso demo iwanai kedo "daisuki" datte uso nanka ja iwanai kara ne aenai toki sae kimi wa nani mo kangaeru yoyuu mo nai kurai kono kokoro o hitorijime ni surun dakara I can't stop my love for you futatsu no ai ga hanarete shimawanai you ni hitotsu ni daitete sore demo fuan na yoru wa otozureru mono dakara ne sonna toki wa gyutto oogesa ni dakishimete ite kakegae no nai mono kimi ga takusan koko ni kureta dakedo nani yori mo ichiban kakegae no nai mono wa kimi nan da I can't stop my love for you futari katatta mirai ni kimi to no ibasho ga aru you ni Oh baby I can't stop my love for you kimi no subete ni natte ne tsunaida te shinjite aruite yuku to chikau yo

中文歌词： I can't stop my love for you 在我俩所诉说的未来里就像有着与你共处的栖身地一般 Oh baby I can't stop my love for you 请把我当成你的全部喔相系的手相信着我走下去发誓喔在我俩的眼前像谜般扩大的未完成地图可是非常非常心动……即使我尝试着用尽所有话语都无法诉说我的思念这个爱是悄悄的诉说喔爱的意义 I can't stop my love for you 因为我俩走路的速度应该一定不会是相同的才对啊如果当你的身边这相系的手离去时为了不失散你一定得要来接我喔虽然「最讨厌你了」这句话即使说谎也不说但是就是因为「最喜欢你了」这句话虽然不是说谎也不说啊连无法见面的时间你就像都无法思考任何其它东西般因为这个心你想独占 I can't stop my love for you 为了不让两个爱分离请将它抱在一起即使如此不安的夜晚也会来临的啊到时候请你要紧紧地夸张地抱住我喔无法替代的东西你在这里给予我很多话虽如此比任何东西都还重要无法替代的东西是你 I can't stop my love for you 在我俩所诉说的未来里就像有着与你共处的栖身地一般 Oh baby I can't stop my love for you 请把我当成你的全部喔相系的手相信着我走下去发誓喔日文歌词： I Can't Stop My Love For You 2人语った未来に君との居场所があるように Oh baby I can't stop my love for you 君のすべてになってねつないだ手信じて歩いてゆくと誓うよ 2人の目の前谜めきひろがった未完成な地図でもとてもとても心动いた…どんな言叶并べてみても语りきれない想いこの爱がささやくよ爱しさの意味を I can't stop my love for you 2人歩むスピードはきっと同じはずじゃないからねもしも君のとなりこの手が离れたときは见失わないようにきっとむかえに来てね大嫌い？

Phage display of recombinant human lymphotoxin mutation librariesand receptor affinity screeningContraction of conformable large molecule mutation library is pivotal for molecular evolution study in vitro. In order to extend the range of the screening region of amino acid in LT gene, several receptor binding sites of LT were mutated randomly by overlap PCR amplification with the random nucleotide primers, the R46, S106, L130 combined site-directed random mutation library and R46~A52, S106~F110, R46~A52 + S106~F110 region random mutation libraries of rhLT were constructed successfully.

二。重组人淋巴毒素噬菌体库的构建及受体亲和筛选构建适合的大容量生物大分子变异体文库是进行体外分子进化研究的关键，为了扩大LT基因序列中被筛选的氨基酸范围，我们采用含随机核苷酸序列的引物，通过Overlap PCR的方法对LT的受体结合区域进行多点随机突变，分别构建了rhLT R46+S106+L130三点随机突变组合文库和R46～A52、S106～F110、R46～A52+S106～F110区域随机突变体库。

Results The DNA was obtained from PPJ through a nasopancreatic tube. Aberrant p16 methylation and K- ras gene mutation were detected in the same samples of PPJ. Sensitivity,specificity,positive predictive values,negative predictive values and accuracy of HE staining for pancreatic cancer were 40%,100%,100%,45.4% and 60.0%,respectively. Of the 20 cases of pancreatic cancer,K- ras gene mutation was detected in 14 (70%) and the p16 gene was shown to be methylated in 7 (35%). Of the 8 cases of chronic pancreatitis,K-ras gene mutation was detected in 2 (25%). Of the 2 cases of mucinous cystoadenoma of pancreas,K-ras gene mutation was detected in 1 (50%). Aberrant methylation of p16 was not detected in pancreatic juice samples from patients with chronic pancreatitis and mucinous cystoadenoma of pancreas.

结果 所有胰液标本均成功抽提出DNA，30例胰腺疾病病人胰液标本同时进行了K-ras基因突变和p16基因启动子区5′CpG岛甲基化检测，其中20例胰腺癌病人胰液中K-ras基因突变率为70%（14/20），p16基因甲基化率为35%（7/20），8例慢性胰腺炎中K-ras基因突变率为25%（2/8），2例胰腺囊腺瘤病人中K-ras基因突变率为50%（1/2），慢性胰腺炎和胰腺囊腺瘤病人胰液中无p16基因甲基化。

Objetive:To investigate the histopathological features and detect the mutation of JAK2V617F in PV、ET and IMF;to analyze the effects of the stages of myelofibrosis and the mutation on the number of leucocyte in PV and ET patients;Methods: the morphological features of 69 patients who dignosed in the first time were studied by HGF and Gomori stains ; the mutation of JAK2V617F was detected in 38 blood or marrow samples of PV、ET and IMF patients by real-time PCR;the effects of the stages of myelofibrosis and the mutation on leucocytes were analyzed;Results: Different stages of reticulin were observed in 15 PV and 15 ET patiens'marrow sections,and the cytosis、the pleomorphism and the bone trabecula side location of megakaryocytes were observed in all PV、ET and IMF patients;the means of leucocyte in patients with and without JAK2V617F were 20.4×109/Land 11.4×109/L respectively;the P value was 0.367 as comparing the leucocytes among different stages of reticulin increasing.

目的：探讨PV、ET、IMF患者的骨髓病理特点并且检测JAK2V617F突变的发生率，分析骨髓中纤维增生程度以及JAK2V617F对PV、ET患者白细胞数的影响；方法：69例初诊患者的骨髓切片经HGF染色以及Gomori染色后进行形态学研究；并利用real-time PCR法测38例患者的骨髓或者外周血的JAK2V617F突变；分析骨髓切片中纤维组织增生程度以及JAK2突变对患者白细胞数的影响；结果：15例PV和15例ET患者的骨髓切片中可以观察到不同程度的纤维组织增生，所有PV、ET、IMF患者骨髓病理中可见到巨核细胞增多、巨核细胞多形性改变以及巨核细胞定位于骨小梁旁；12例PV、4例ET、4例IMF中检测到JAK2V617F；有突变和无突变患者发病时白细胞数均值分别为20.4×109/L和11.4×109/L，比较不同纤维增生程度的PV、ET患者的白细胞数均值，P值为0.367。

The results were as follows. In the insert mutation genotypes, there was significant difference between body weights of 2 weeks aged BB and AB genotype (P.05), while there was no significant difference between AA and other genotypes. In the mutation genotypes, there was no significant difference in body weight. Insert mutation loci had no significant effect on fossil bone length and shank length of 0-12 weeks aged chickens, only mutation loci had a significant effect on fossil bone length and shank length of 6 weeks aged chickens.

分析多态位点与0-12周龄鸡生长发育性状的关联性，结果表明：插入突变基因型中，2周龄鸡体重BB型与AB型差异显著(P.05)，但AA型与AB型和BB型差异均不显著，而突变各基因型间没有显著差异；插入突变位点对0-12周龄鸡的龙骨长、胫骨长均没有显著影响，而突变位点仅在鸡6周龄时表现出与龙骨长、胫骨长间的差异显著性。

The teacher likes the honeymouthed little girl very much.

老师很喜欢这个嘴甜的小姑娘。

Mr. Notker Bien's interests are traveling, spending quality time with the family and long-distance-running.

诺特卡·柏恩先生热爱旅游，长跑，以及和家人一起共度美好时光。