查询词典 mutagenesis

Several hrp mutants, which losted their ability to trigger hypersensitive response on nonhost plant tobacco and to be pathogenic on rice, were produced from Xanthomonas oryzae pv.oryzicolaby chenmical mutagenesis.

化学诱变水稻条斑病细菌（Xanthomonas oryzae pv.oryzicola，Xooc），获得了在水稻上丧失致病性和在非寄主烟草上丧失激发过敏反应的hrp基因突变体。

OLIGOMAP - OLIGOMAP is a tool to help researchers engineer restriction sites into oligonucleotides using silent mutagenesis and reverse translation algorithms.

OLIGOMAP是一个帮助研究人员使用哑诱变和反向转换算法来约束寡核苷酸位置点的工具。

Unshedule DNA synthesis test is a very important indicator which is used to detect the mutagenesis and carcinogenesis of chemical carcinogens.

非程序DNA合成试验已被用作检测化学物质的致癌性/诱变性，筛选化学致癌物的一项重要参考指标。

The dose-dependent experiment revealed that Id1 antagonised E47 and Ets1 mediated transcriptional activation in a dose-dependent manner. With pGL3-870 as a template, site-directed mutagenesis of E-box and / or ETS-binding site was introduced into p16 promoter. E47 could not activate the transcription of p16 promoter with E-boxes mutated, and Ets1 could not activate the transcription of p16 promoter with EBS mutated. The synergy effect between E47 and Ets1 would occur only if the E-boxes and EBS were present. These facts demonstrated that E47 and Ets1 proteins were not recruited to the promoter by binding to other cis-elements or through interaction with other protein factors, however, they bound to DNA directly through respective consensus sequences in p16 promoter.

以pGL3-870为模板，构建了E-box或/和EBS点突变的p16启动子报告基因载体，将野生型和突变型的报告载体分别与pI-E47、pI-Etsl以及pI-E47+pI-Ets1共转染，发现E47和Ets1所诱导的转录激活作用依赖于启动子DNA上存在的各自的特异结合序列，二者的协同作用也只有当两个结合位点都存在时才会发生，说明E47和Ets1不是与其它作用元件或其它蛋白质因子相作用而被招募到该启动子的，它们是通过启动子上各自的特异结合位点与DNA直接结合的。

And then re-use the nitroso muscle strain mutagenesis, in order to collect acid analog of GABA as a selection factor has been a 18% increase in production of new strains 42,, this new high-yield flask under the conditions of strain in the process were studied.

然后又对该菌株再采用亚硝基肌诱变，以撷氨酸结构类似物氨基丁酸作为筛选因子又得到一株新的产量提高18%的菌株42，，对此新的高产菌株在摇瓶条件下的工艺进行了研究。

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

A SV40 based shuttle vector pSP189 and African green monkey kidney cell line was appied , which constitute a shuttle vector /mammalian cell system to detect mutagenesis in vitro induced by animal drugs olaqiundox and quinocetone.

本研究将基于SV40病毒的短暂复制型穿梭质粒pSP189与非洲绿猴肾Vero细胞组成穿梭质粒／哺乳动物细胞诱变检测系统，并将该系统应用于兽药喹乙醇和喹烯酮的诱变性检测。

Molecular cloning and expression of FKN/CX3CR1 and their mutated counterpartsTo study the evolutionary rule in function from Old World Monkey to Homonid and the role of negative selection sites, possible Darwinian positive selection sites and deletion mutation, full-length FKN/CX3CR1 and mutant gene were obtained by overlapping extension PCR and site-directed mutagenesis.

FKN／CX3CR1分子及突变子的基因克隆和表达克隆旧大陆猴和人猿超科7个物种FKN、CX3CR1全基因及其突变体并进行体外表达，为后续观察FKN／CX3CR1在高级灵长类物种进化过程中免疫学功能的演变并在功能上验证FKN／CX3CR1协同进化以及研究上述位点对FKN／CX3CR1介导的免疫学功能的影响奠定分子生物学基础。

I am sure after a few weeks of adaptation and familiarization I will have no difficulty in functioning on a daily basis in an English speaking society or in participating fully in graduate studies.

在大学的三年的学习生活不仅给予我自身的充实，更重要的是磨练和培养了我较强的自觉能力和严谨，踏实的作风以及不怕困难的精神，对未来充满了信心！

As the first volcanoes erupted, one of the gases that bellowed out was steam.

在第一批火山喷发时，喷出的气体之一就是水蒸汽。