查询词典 medium deep

The strains could overgrow in CMC Na-Congo red medium and tobacco stem powder medium within 2~5 days.

菌株在2~5d内可长满CMC-Na-刚果红平板和烟杆粉末培养基。

Eyes are brown, medium in size, oval in contour, bright and clear and overhung with medium length eyebrows.

眼睛：褐色，中等大小，卵形轮廓，明亮而整洁，下垂的、中等长度的眉毛。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The additional supplements including glucose,ascorbic acid and insulin had the enhancement effects on C.andersoni development,while folic acid had no significantly effect on C. andersoni development and calcium pantothenate had inhibited effect.When added 50 mmol/L glucose,50μg/ml ascorbic acid,0.3 U/ml insulin into 10%FCS RPMI-1640 medium,the proliferation of C.andersoni had a great significantly increase,and the gene copies of C.andersoni were 8-fold compared with that in 10%FCS RPMI-1640 medium.

冻融法+蛋白酶K法+声裂法提纯隐孢子虫卵囊DNA效果最佳，其次为冻融法+声裂法和蛋白酶K法+声裂法，冻融法+蛋白酶K法效果最差。10%FCS是培养安氏隐孢子虫的最佳FCS浓度，葡萄糖、维生素C和胰岛素都具有促安氏隐孢子虫增殖作用，泛酸钙无促虫体增殖效果，而叶酸则抑制虫体增殖。10%FCS RPMI-1640培养液中加入50 mmol/L葡萄糖，50μg/ml维生素C和0.3 U/ml胰岛素促安氏隐孢子虫增殖效果最显著，虫体增殖8倍。

The path or one of the set of paths, parallel to the reference edge on a data medium, associated with a single reading or writing component as the data medium moves past the component .

与数据媒体基准边并行的轨道或一组轨道中的一条，当数据媒体移动而经过单个读元件时，就与这种读元件相联系。

The results showed as follows: 3mg/LBA in differentiation medium was favorable for regeneration of callus; addition of 3mg/LABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mg/LBA对分化有利；继代培养基中加入3mg/LABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

The results showed as follows 3mgLBA in differentiation medium was favorable for regeneration of callus; addition of 3mgLABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mgLBA对分化有利；继代培养基中加入3mgLABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

In this paper, an analysis of Linyi City Lanshan district the status of small and medium enterprises financing difficulties and the emergence of the problem, focused on financing small and medium-sized Lanshan district Linyi several difficult issues an effective way, including the self-construction companies, banks and the Government of the corresponding support and improve the information technology business, as well as the development of Lanshan district pawn broking and security industries.

本文主要分析了某区中小企业的现状及其出现融资难问题的原因，着重阐述了某区中小企业融资难问题的几条有效途径，包括完善企业自身建设，银行和政府的相应支持，提高企业的信息化，以及发展兰山区的典当业和担保业等。

However, as China's pawnbroking industry has experienced ups and downs in the growth and development its social function from the past to save the poor personal urgency to save individuals and small and medium-sized enterprises. not only to solve the problem of funds from the settlement of the fund issue, short-term financing small and medium-sized enterprises but also become an important organization. Pawnbroking 2001 made major changes to the nature of this new financing channels for more spacious, Compared with the banks increasingly significant advantages.

然而，随着典当业在我国历尽波折的成长和发展，其社会功能已由以往的救个人之穷转变为救个人和中小企业之急，由原先仅仅解决资金问题发展到不仅解决资金问题，而且还成为中小企业开展短期融资的重要机构。2001年典当业性质的重大变更使得这种新的融资渠道越来越宽阔，较之银行的优势也日益显著。

But law environmental problem of development of small and medium-sized enterprises perplex and hinder further development of small and medium-sized enterprises seriously.

但是 ，中小企业发展的法律环境问题却严重困扰和阻碍着中小企业的进一步发展。

In this new architectural plan the Garden of Golgotha came to be at the centre of the new city.

Aelia 是高举他自己，并在此城内朝拜罗马诸神。

The continuous nature of peat accumulation means that terrestrial coaly rocks may provide a higher resolution and more complete record of base-level change than the time-equivalent shallow-marine strata, thus enabling the identification of highfrequency cycles that are not recorded in themarine strata.

泥炭积累的持续性质的手段，陆地coaly岩石可提供较高的分辨率和更完整的记录基准面变化的影响时，相当于浅海阶层，从而使确定的高频周期，不记录在themarine 阶层。