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Albicans strains with fluconazole. Method: A series of 16 strains C. albicans from the same body were induced to form pseudo-hyphae in RPMI 1640 medium and DMEM medium at 37 癈 for twenty-four hours respectively. The pseudo-hyphae and yeast were counted and the ratio between pseudo-hypha and total cells was calculated. C. albicans strains were induced to form pseudo-hyphae in RPMI 1640 medium for seven days by transferring twelve times. The genome DNAs of the hyphal-form of C. albicans was cleaved by two restriction enzymes EcoR I and Bgl II, the products were electrophored and photoed.

以同一母体来源的白念珠菌CA-1～A-17(共16株)作为研究对象，采用RPMI1640和DMEM培养基，37℃培养24h诱导其假菌丝形成，记录不同观察时间时的菌丝相和酵母相细胞数，计算菌丝相白念珠菌形成率；以RPMI1640培养基，37℃连续传代培养7天(转种12次)后，分离菌丝相白念珠菌并提取其基因组DNA；以EcoRⅠ和BglⅡ消化菌丝相基因组DNA，消化产物进行琼脂糖凝胶电泳并拍照；用随机引物(5'…TCACCACGGT…3')扩增菌丝相基因组DNA，采用变性聚丙烯酰胺凝胶电泳分离扩增产物并拍照。

Second, based on the spherical vector wave function in uniaxial anisotropic medium, and the first, second, third and fourth spherical Bessel functions satisfy the same differential equation and recursive formula. The scattering fields in terms of spherical vector wave function from a uniaxial anisotropic spherical shell and an anisotropic uniaxial-coated conducting sphere by a plane wave are derived. The electromagnetic fields in uniaxial anisotropic medium and free space can be expressed in terms of spherical vector wave functions in uniaxial anisotropic media and isotropic medium.

二、在建立均匀各向异性单轴介质球矢量波函数理论的基础上，利用二阶线性偏微分方程的性质和第一、第二、第三和第四类球Bessel函数满足相同的微分方程和递推关系，我们分别研究了单轴介质球壳和单轴介质涂覆导体球对平面波的电磁散射特性，首先给出了各个区域的电磁场用球矢量波函数来表示的解析表达式，进而利用电磁场在边界上满足电磁场切向连续的边界条件和球谐函数的正交性，得出了各向异性单轴介质球结构中电磁场用球矢量波函数表示的系数所满足的矩阵方程。

Where sterility test media are to be used in the Direct Inoculation of the Culture Medium method under Test for Sterility of the Product to be Examined , modify the preparation of Fluid Thioglycollate Medium and the Soybean–Casein Digest Medium as follows.

当无菌检查培养基用于供试产品无菌检查项下的培养基直接接种法时，按如下内容变更巯基醋酸盐液体培养基和大豆-酪蛋白消化物培养基的制备方法。

The connection of Literature and medium is more and more tightness in the mass media age, and attended widely by academia. The academician discussed deeply the influence of medium to literature, developing progress and so on. But they had less attention at the influence of literature medium to accepters, especially about their aesthetic change.

大众传媒时代文学和媒介的关系日益紧密，并为学界广泛关注，学者从媒介对文学产生的影响、发生过程等多方面进行了深入地探讨，但是在文学媒介对受众的影响，尤其是受众审美转变方面关注不够。

In the end, I put forward how to solve the good and bad influences of literature medium to accepters' aesthetic, and put forward extant language and character how to existing continue affected by mass medium and network., and resolve how the accepters can make use of medium better but not controlled by it via improving their spiritual ambit and aesthetic level.

最后，提出如何应对文学媒介对受众审美的利弊影响，在大众媒介和网络媒介的冲击下，原有的语言、文字媒介如何继续生存下去，受众如何通过提高自身的精神境界和审美素养来更好地利用媒介而不是被媒介控制。

Results The pyronaridine-coated plate and culture medium were proved to be stable. The effective period of pyronaridine-coated plate, the ampule sealed liquid culture medium and the bottled lyophilized culture medium, all stored at 4 ℃ was 6 months, 2 months and 2 years respectively.

结果 研制的咯萘啶涂药板及现场用的培养基效果稳定，咯萘啶涂药板4 ℃贮存有效期为6个月，4 ℃贮存的安瓿封装液体培养基及瓶装冰冻干燥培养基有效期分别为2个月和2年。

There were also conspicuous accumulations of membrane bound esicles, dense bodies, myeloid figures, and areas of esicular in Schwann cells. These changes were seen with dialyzer exposed medium or with medium containing patient serum but not with controls or with medium incubated in plate dialyzers.

这些变化见于使用了暴露于透析器的培养基的DRG，或使用含有病人血清的培养基的DRG，但变化不发生于使用对照用组织培养基或者在平板透析膜中孵育的DRG。

Electron microscope findings were compared between DRG grown in control medium containing 10% calf serum, DRG incubated in the same medium exposed to the blood compartment of capillary flow or plate dialyzers and DRG grown in medium supplemented with 10% dialysis patient serum.

对下列基质中生长的DRG的电镜发现进行比较：即DRG在含有10%牛血清的对照基质中生长、然后在暴露于毛细流血隔室或平板过滤膜（ blood compartment of capillary flow or plate dialyzers）的相同基质中孵育；DRG生长于加有10%血透病人血清的组织培养基内。

To achieve this, the or each steam generator pipe (22, 50, 60) has a respective downpipe section (34, 52, 62, 64), which is approximately vertical and through which the flow medium can flow downwards and a respective riser pipe section (36, 54, 66, 68) connected downstream of the downpipe on the flow medium side, which is approximately vertical and through which the flow medium can flow upwards.

为达到此目的，按本发明这一根或每根蒸汽发生器管(22、50、60)分别包括一个近似垂直布置可被流动介质沿向下的方向流过的下降管段(34、52、62、64)和一个沿流动介质流向连接在该下降管段下游的、近似垂直布置并可被流动介质沿向上的方向流过的上升管段(36、54、66、68)。

The new nonlinear percolation law can be used for study of gas nonlinear percolation theory in low permeable medium and engineering application. It provides the theoretical ground for the development of gas resource in low permeable medium and the geological evaluation of low permeable medium environment, and makes the important reference for practical engineering application of many fields.

新的非线性渗流定律可运用于低渗透介质气体非线性渗流理论及工程应用研究，为低渗透介质天然气资源开发与低渗透岩石介质环境地质工程评价奠定了理论基础，对多种领域的工程实际应用具有重要指导意义。

It's not quite as simple as that.

并没有那么容易。

It debuted in 1992 in Japan and the United Kingdom, and 1993 in the United States.

它首次亮相于1992年在日本和英国，在美国1993年。