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The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

So it needs improving or alternating urgently. Some good germplasms for rootstocks for citrus have been picked out and introdcted into. Some somatic hybrids used as rootstocks have been selected through somatoplasm fusion. Some methods in early identification on stability of inherence and copatiblity and dwarf are explored, and they need improving. Rootstocks having some virtues have been selected, but they cannot take the leading place of trifoliate orange. The research method that the rootstocks are only compared with each other should be altered into that the rootstocks and given control measures or environmental conductions are connected to make the rootstocks compared with each other.

在砧木选择研究进展上，中国调查出和引进了一些优良的柑橘砧木种质资源，通过体细胞融合选育出一些可作为柑橘砧木的柑橘体细胞杂种资源；在柑橘砧木资源的遗传稳定性、嫁接亲和性和矮化性等早期鉴定上，探索出一些有待完善的鉴定方法；在砧木选择上，选出一些具有某些优点的砧木，但尚不能冲击枳砧的主导地位，并且选择研究方法有必要把现行单纯的砧木比较改为与砧木种类、栽培技术或环境条件相结合进行比较。

The part that I'm taking on isn't exactly clear at the moment. I know I will be one of the ensemble..and possibly get a few lines here and there....The show already has it's lead roles casted and the other roles are not suitable for my age. But, I don't mind...it will be the first time that I actually get to be a part of a professional Broadway production! Everything is coming from NY!!! And the original writer is going to be the director! So, I know that this will be my chance to learn and make my way into the industry. Hopefully, next time, there will be a bigger role for me to play.I'm so thankful that God has been leading me into the right direction. I am so blessed with opportunities...and I know that my patience is finally paying off. I'm very excited to be able to train more in tap dancing because I have always wanted to improve in that area....it's going to be a lot of hard work..but, I'm so looking forward to it. I do feel very sorry for my fans who have been walking with me since the beginning of the competition. They have been so supportive of me and it made me so sad to see them cry when I made my decision. I hope that they will not be upset with me because of it. I hope that they will see that this choice will only help my future...and I hope that they will continue to support me even though my participation with the competition has come to an end. It really touched me to see that they cared so much.

我知道我可能只是其中一个群舞，或许会有点台词，因为他们早就订了女主角人选，另一个女角年纪比较大，并不合适我，但是我不在乎…这是我的第一次参于真正百老制作的演出，每一个成员都是从纽约来的，原编剧将会亲自来导演这次演出，所以我很清楚这是我最好的学习机会，也是给我提供了一条通向百老的捷径之路，下一次我一定会争取到一个很好的角色…我感激我的上帝指引我一个正确的方向，我非常感激有这麼好的一个机会，我等了那麼久，总算有了一个好的起步…我特别兴奋的是，我这下子总算有机会好好学"踢躂舞"了，这是我最弱的一种舞，我一直希望能把它学好，尽管我知道会很辛苦，但是我已经作好了思想准备…还有对那些从一开始就支持我的那些粉丝，我感到非常抱歉，尤其是当我在台上说出我离去的决心时，看到她们一个个都哭得那麼伤心，我也很难过，我希望她们能明白，这个决定是绝对对我前途有利的，我希望就算是我不再比赛，她们仍然会支持我，一直以来，她们对我的关心和鼓励最让我心动的…。

So why not turn suffering into praising life, and turn tears into the light in your heart, only in this way can we make it through and hold the magical spark to light the fire of life.

为什麼不把痛苦锤炼成对生命的赞扬，把眼泪作为心灵的灯盏呢？只有这样才会真正寻找到快乐，一展生命中灿烂的光泽！

Include to entering into an election contest to one's heart's content when, the grandmother that he leaves working station to visit his such thing, also by ably fashion into most make moving affection a ring of light, touched countless Everyman with heart of a dutiful son.

包括在竞选正酣的时候，他离开工作岗位去探望自己的外婆这样的事情，也被巧妙地塑造成最打动人的情感光环，以一颗孝子心感动了无数普通人。

Make use of small diameter twist drill to change it into drill of wearing intersect holes and put it into the ability education of locksmith.

利用小直径麻花钻，改磨成"排孔钻"，并运用于技工学校的钳工专业技能培

In a medium saucepan,melt the remaining 2 tablespoons butter.stir in the turkey and flour.add the milk,and heat through.stir the turkey mixture into the vegetables mixture,and cook until thickened.cool slightly,then pour mixture into the unbaked pie shell.roll out the top crust,and place on top of filling.flute edges,and make 4 slits in the top crust to let out steam.

取一只中等炖锅，融化其余2大汤匙黄油。交半年火鸡和面粉。加牛奶，热透。把火鸡混合物搅拌进蔬菜混合物，熬至浓稠。微凉，然后再把混合物倒进未烘烤的派皮里。辗平最上面的派皮，放在最上面的馅上。凹槽边缝，在最上面的派皮上做4个狭缝，以使蒸汽释放。

The researcher provides suggestions for further development in the following eight respects:(1) promote the union catalog of ISBNnet to libraries with limited human resources so as to improve the administration efficiency;(2) integrate the personalized service functions into the library acquisition system in order to provide customized alert services for new publication information;(3) embrace RSS into the library acquisition system to make it easy for library users to request real-time purchase recommendation;(4) add the mechanism for group learning to the library acquisition system to facilitate the sharing of professional knowledge and interaction;(5) appeal to the Central Trust of China in Taiwan to amend the regulation on order arrival rate in the Inter-Entity Supply Contract;(6) advocate the union catalog to include bibliographic records of publications in languages other than Chinese;(7) encourage the library to launch more practices of e-services to enhance administrative efficacy;(8) improve the quality of other library services by employing the action research method in library practices.

本研究并提出八点建议：(1)将全国新书资讯网联合出版书目推广到人力资源缺乏的学校，以提升行政效能；(2)联合出版书目的模式，可推行到其他语文领域；(3)图书采访系统可纳入个人化功能，便於整理个人书讯；(4)图书采访系统可纳入RSS功能，便於读者的即时荐购；(5)图书采访系统可纳入群体学习机制，彼此分享、互动成长；(6)中央信托局的中文图书共同供应契约应纳入到书率的规范；(7)图书馆应导入更多的e化服务，以提高行政效能；(8)应用行动研究法於推动图书馆其他业务的改善，以提升服务品质。

Probing into the legal force questions about voting trust, the purpose is to make the voting trust system into the real life so that it can play a positive role in the social economy of our country.

探讨股东表决权信托的法律效力问题，目的是让股东表决权信托制度能真正地进入我国的现实生活，在我国社会经济中发挥积极作用。

Wood Vinegar is a natural material that is distilled from wood. It is compose of organic acid and hydroxybenzene , Various dosage of wood vinegars, pyrolignerous acid and guaiacol were mixed into organic manure firstly in this experiment, and then, the above said organic manure are separately added into soil. Under the analysis of the amount of the edaphon, adding 10g wood vinegar to 1kg organic manure and 30kg soil can much active the edaphon effectively in a short term and make a maximum rising extent of edaphon numbers.

木醋液是从多年生柞木中经过高温蒸馏出来的纯天然物质，其主要成分是有机酸和酚类物质，为了明确木醋液和主要成分对土壤微生物的作用，将加入不同剂量的木醋液、愈创木酚、醋酸的有机肥施入土壤，观察土壤中微生物数量的变化，结果表明：在1kg有机肥中加入10g木醋液，施入30kg土壤中，土壤微生物数量增加幅度最大，土壤微生物10天内被有效地激活；同时，当在1kg有机肥中木醋液的添加量高达50g时，对土壤微生物有一定的抑制作用。

There had been a moment during the breakfast meeting, though, after the backslapping and the small talk and when all of us were seated, with Vice President Cheney eating his eggs Benedict impassively and Karl Rove at the far end of the table discreetly checking his BlackBerry, that I witnessed a different side of the man.

那次早餐会期间，在表示过关心和寒暄以后，我们所有人坐了下来，我注意观察了一下，副总统切尼面无表情地吃着班尼迪克蛋（Eggs benedict，源自美国，以英式松饼、火腿、水煮蛋以及荷兰酱组合而成），卡尔。罗夫在餐桌的远端谨慎地经常查看他的黑莓手机，我目睹了他所不为人知的一面。

Talk Undelete failed; someone else may have undeleted the page first.

Talk 无法删除选定的页面或图像（它可能已经被其他人删除了）。