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ABSTRACT Antitumor Activity of Dendritic Cells Activated with gP96 Peptide Complex Department of Thoracic Surgery, Peking University People's Hospital Objectives: To investigate the expression level of gP96 in human lung cancer samples; purify gP96-polypeptide from human and mouse lung adenocarcinoma cell lines; prepare tumor vaccine by in vitro gP96-polypeptide stimulation of monocyte-derived dendritic cells and test its efficiency both in vitro and in mice models.

研究人肺癌组织热休克蛋白gP96—多肽复合物的表达水平;肺癌细胞株来源的肿瘤组织gP96—多肽复合物的提取和鉴定;外周血来源树突状细胞培养和鉴定:将gP96—多肽复合物和DC体外共同孵育制备疫苗,在体外激活T淋巴细胞的能力及在小鼠体内的抑制肿瘤生长作用的研究。

From 1 to 7 weeks PC MG could be isolated from laryngeal swabs in all the vaccinated groups and the unvaccinated,but the isolation rate was lower in the former.7 weeks PC MG could be isolated from trachea,lung and air sac in the control group,while in Group I MG was isolated only from trachea and lung.

攻毒后第7周从对照组鸡的气管、肺、气囊中均可分离到MG,而试验Ⅰ组仅在气管和肺中分离到了MG。

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活,ICAM-1表达升高,与被激活的PMN表面表达的LFA-1相结合,"PMN-EC"相互作用加剧,一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍;另一方面粘附的PMN过度吞噬或激活,当白细胞吞噬的颗粒不能被封闭隔离,连同细胞内的酶被释放出来,其中的PMN-elastase能够降解细胞基质中各种成分,水解多种蛋白,加重胰腺的毛细血管内皮细胞和腺泡的损伤;释放的溶酶体酶使组织蛋白水解,产生的不饱和脂肪酸引发脂质过氧化方应,破坏细胞膜的结构和功能;释放的炎性细胞因子,激发其他的免疫细胞的功能,导致进一步的组织损伤和坏死,加重SAP的病理损伤,最终导致休克、脓毒血症及多器官功能障碍等严重后果。

Comparisons between the cell lines were made on the basis of time to observed cytopathic effects, ultra structure changes, titer in 50% tissue culture infectious doses, and the alterant distribution of DNA content in cell cycle. The results showed that, under identical conditions, various degrees of CPE could be found in the Hep-3B, A549 and NIH 3T3 36h post infection of BTV-10, while there was no CPE could be observed in HEL.

随后在对BTV-10体外感染特性的研究中,我们在相同条件下,将该病毒接种于1株原代人胚肺成纤维细胞(Human embryonic lung fibroblast cells,HEL)和3株传代细胞株:人肝癌细胞(Human hepatic carcinoma 3B cells,Hep-3B);人肺腺癌细胞(Human lung carcinoma A549 cells,A549);鼠正常成纤维细胞(Normal mouse fibroblastscells,NIH 3T3)。

We hypothesized that lung injury and inflammation in ventilation-induced lung injury are related to angiotensin Ⅱ.

我们假设机械通气导致的肺伤害与肺脏的血管收缩素有关。

Results The extract of Huanglian Xiangru Decoction with different proportions of herbs could inhibit pathological changes in lung of mice caused by FM1 strain of Influenzavirus A and lower lung index, indicating anti-influenza activities in vivo with antifebrile effects on febrile rats caused by yeast, resistance to acute inflammation of auricular swelling of mice caused by paraxylene, decreasing writhing times of mice caused by glacial acetic acid, and increasing pain threshold of mice caused by hot plate.

结果 不同比例的黄连香薷饮提取物可明显抑制甲Ⅰ型流感病毒FM1株引起的小鼠肺部病变、降低肺指数,有体内抗流感病毒作用,对酵母所致发热大鼠有退热作用,对二甲苯所致小鼠耳廓肿胀有抗急性炎症的作用,有减少冰乙酸致小鼠疼痛扭体次数和提高热板致痛小鼠痛阈值的作用。

Objective:To investigate the effects of antimalarial artemether on the proliferation of human lung adenocarcinoma cell line A549 in vitro and provide experimental data for the treatment of lung cancer with artemether.

目的:研究抗疟疾药物蒿甲醚对人肺腺癌A549细胞株体外生长的影响,为蒿甲醚治疗肺癌提供实验依据。

And lung cancer and inflammation were the main reasons to cause lung atelectasis in the middle leaf on the right.

病变部位以右中叶最多共26例(30.6%),肺癌和炎症是右中叶肺不张的主要病因。

If there is long-term smoking before patient operation history, or when the operation, the catch cold catch cold after the operation, cold, all can make bronchus autacoid grow in quantity, easy complication of the lung after causing art, be like pneumonic, lung not piece etc.

假如病人手术前有长期吸烟史,或手术时、手术后受凉、感冒等,均可使支气管内分泌物增多,易引起术后肺部并发症,如肺炎、肺不张等。

Tissue Preparation: 159 cases of paraffin sections of lung carcinomascomprised of adenocarcinoma (84 cases), squamous cell carcinoma (42 cases), smallcell lung carcinoma (10 cases), large cell carcinoma (15 cases), pleomorphic carcinoma(4 cases), and metastatic colon carcinoma (4 cases), and 86 adenocarcinomas fromsome organs other than lung, including colon (15 specimens), stomach (5 specimens),prostate (5 specimens), breast (35 specimens), uterus body (11 specimens), ovary (10specimens) and thyroid (5 specimens) were chosen from the surgical files ofYamanashi University Hospital and Kofu City Hospital.

标本来源:159例肺癌及其癌旁组织标本包括84例腺癌、42例鳞癌、10例小细胞癌、15例大细胞癌、4例多形性癌和4例肺转移性结肠癌,以及86例全身其他组织腺癌及其癌旁组织标本包括15例结肠腺癌、5例胃腺癌、5例前列腺癌、35例乳腺癌、11例子宫内膜腺癌、10例卵巢癌及5例甲状腺癌,均来自日本山梨大学附属医院和甲府市立医院外科手术标本。

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