查询词典 lipase

Starch esterification was proceeded with native cassava starch and three saturated free fatty acids: capric acid (C10:0), myristic acid (C14:0) and stearic acid (C18:0). The fatty acid modified starch was prepared by using lipase PS as the catalyst.

中文摘要本研究以天然树薯淀粉为反应基质，与三种不同链长饱和脂肪酸羊蜡酸(C10：0、肉豆蔻酸(C14：0)与硬脂酸(C18：0)分别於水相及有机相行酯化反应，并且以工业中常用的脂肪水解酶lipase PS催化酯键合成，可得脂肪酸修饰淀粉(fatty acid modified starch， FAMS)。

It was observed after 24hrs of reaction that this lipase could compete very well with lipase from Candida antartica (93. 0％ for isoamyl butyrate synthesis and 95. 0％ for ethyl caproate synthesis) and lipase from Mucor miehei (75. 0％ for isoamyl butyrate synthesis and 93. 64％ for ethyl caproate synthesis).

反应24h后，来自Candida antartica脂肪酶合成丁酸异戊酯和己酸乙酯的转化率分别为93.0％和95.0％，来自Mucor miehei脂肪酶合成丁酸异戊酯和己酸乙酯的转化率分别为75.0％和93.64％。

The different factors involved in the process of the folding and activation of the microbial lipase are reviewed in this article,including lipase-specific foldase,lipase activation factor,prosequence,calcium ion and disulphide bond.

对微生物脂肪酶活性构像的形成和激活机制进行深入研究，有助于我们更好地了解脂肪酶的结构和功能，为脂肪酶基因的大量表达和体外定向进化奠定基础。本文主要综述了作为脂肪酶结构成分的多种因子在脂肪酶活性构像形成、激活及稳定中的作用。具体催化反应体

This finding demonstrates that the regional codon optimization of the lip2 gene fragment at the 5'end can significantly enhance the expression level of recombinant Candida rugosa lipase 2 in the Pichia pastoris system.

这个发现同时也证实了本研究所采用的靠近5'端的局部密码最优化lip2基因片段，对於基因重组 Candida rugosa lipase 2 在Pichia pastoris 系统中的蛋白质表现量具有明显的提升效果。

We use plate to check the lipase activity ,the lipase activity is very low, Compared to the wild type , the mutant could not form the clear halo on the olive oil plate but tributyrin plate, it showed that the 227 th Serine was essential for the lipase activity.

与野生型相比，突变体脂肪酶的酶活很低，不能在橄榄油平板上形成透明圈，但可以在以三丁酸甘油酯为底物的平板上形成透明圈，表明第227位丝氨酸可能对脂肪酶酶酶活具有重要影响。

In this study, isolate 23 microbes with lipase activity from Wu-Jie scrap heap at Ilan and Taichung Environmental Protection Section. When the microbes incubator in 50oC, there are isolates B61 and M63 that lipase activity are better than others. Brevibacillus borstelensis SH168 isolated from NTU resterant food waste compost is used as control check. Isolates B61, M63 and Brevibacillus borstelensis SH168 determine the lipase activity by the LB with tributyrin. The lipase activity of isolates B61 and M63 are 2~3 fold higher than Brevibacillus borstelensis SH168. So isolates B61 and M63 are more useful in producing lipase. Isolate B61 can grow at pH 5~9, and at temperature 20oC ~ 60oC; isolate M63 can grow at pH 6~10, and at temperature 30oC ~ 50oC. Isolates B61 and M63 were identified by 16S rRNA. Isolate B61 is Bacillus circulans, and isolate M63 is Bacillus species.

本研究，於宜兰五结垃圾场和台中市环保局厨余堆肥样品中共分离出 23 株具脂肪酶活性菌株， 23 株菌株中经过三油酸甘油酯的洋菜培养基於 50℃培养 5 天后，发现有 2 株菌的脂肪酶活性较强，分别为分离株 B61 和 M63；以本实验室先前自台大女九餐厅蔬果厨余堆肥中所筛选出的耐高温脂解菌 Brevibacillus borstelensis SH168作为本实验对照菌株，将分离株 B61、M63 及 Brevibacillus borstelensis SH168 3 株菌株於含有三酪酸甘油酯液态培养基培养并测定其脂肪酶活性，发现分离菌株之活性皆比 Brevibacillus borstelensis SH168 高出 2~3 倍，认为分离株 B61、M63 适於产生脂肪酶的菌株，分离株 B61 在 pH 5~9 皆可生长，生长温度介於 20 oC ~ 60oC；分离株 M63 在 pH 6~10 可生长，生长温度介於 30 oC ~ 50oC； 2 株分离菌株以 16S rRNA定序结果分离株 B61 为 Bacillus circulans，分离株 M63 为 Bacillus 属。

The thesis mentioned that the conformation of lipase molecular can be induced by substrate to be an activated one in organic phase through constructing a water-in-oil micro-emulsion system. The activated conformation was recorded using high cross linking degree polymer. The effect of inducement on the activity of recorded lipase polymer was studied, the catalyzed property of recorded lipase polymer in water phase was investigated, and the fluorescence spectrum of lipase solution and recorded lipase polymer under different kinds of denaturalized circumstance were analyzed.

本文提出通过构建油包水型微乳体系，使酶分子能够在有机相中被反应底物诱导出活性构象，利用高交联度聚合物对该构象进行刻录，比较了底物诱导对刻录酶活性的影响，考察了刻录酶在水相中催化特性，并对脂肪酶溶液和刻录酶聚合物在各种变性条件下的荧光光谱进行分析。

To examine the hypothesis, we test whether maize beta-amylase can serve as a lipase inhibitor.In order to conduct the interaction assay of beta-amylase and lipase, we adopted a preparative electrophoresis method with a Prep Cell to purify beta-amylase proteins from germinating maize endosperms and soybean cotyledons. We pre-mixed tested proteins with lipid substrate prior to the addition of lipase preparation and assayed the lipid hydrolytic activity.

本实验利用Prep Cell纯化玉米萌芽谷粒和大豆子叶中的beta-淀粉酶，分别将两种不同物种的beta-淀粉酶与胰脂解酶预先反应后，再加入脂解酶反应液中观察活性的变化，结果发现脂解酶的活性未如预期，没有抑制的效果产生，将甘薯beta-淀粉酶以同样方法与胰脂解酶作用，结果同上述两种beta淀粉酶依旧没有抑制的现象。

Lipase is one of the most widely used enzymes in industries such as leather, food and bio-diesel production.

脂酶(Lipase， EC3.1.1.3)是普遍应用于皮革、饲料及生物柴油工业的工业酶制剂，具有广泛的应用价值。

Novozym 435 was used for the synthesis of vitamin A plamitate. The influences of solvent, the molar ratio of substrates, the reaction temperature and time, and the water concentration were optimized and the best result was obtained by interesterification from 0.200 g vitamin A acetate and 0.468 g palmitic acid, at 30℃, in 10 mL hexane, containing 0% of water, with 10% of lipase(mass ratio, immobilized lipase to vitamin A plamitate). In these conditions, 75% of vitamin A acetate was converted into vitamin A plamitate. The immobilized lipase was reused about 6 batches.

对催化合成维生素A棕榈酸酯反应的脂肪酶和反应介质比较，同时对影响合成维生素A棕榈酸酯反应的因素（温度、初始水含量、底物摩尔比、反应时间和酶量等）进行了探讨，优化了反应条件：在10 mL不含水分的正己烷中，0.200 g 维生素A醋酸酯和0.468 g棕榈酸在酶量为10％（指固定化酶与维生素A醋酸酯的质量比）的固定化脂肪酶催化下，在 30℃、190 r/min下反应6 h，转化率可以达到75%，固定化酶可连续使用6次以上。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。