查询词典 ligated

Methods Thoracic aorta of Wistar rat is obtained, by using a specially made crochet hook, the aorta is over turned, then ligated the two ends, the vascular endothelial cells are wiped off with 0.1% collagen Ⅰ enzyme for 15 minutes.

将大鼠主动脉翻转，两端结扎，用0.1%胶原酶消化血管内皮细胞，剩余血管剪成小块均匀种植于培养瓶底，加入DMEM培养液培养，观察血管平滑肌细胞的生长。

Methods Fifty-two Wistar rats were randomly assigned to three groups: sham operated, bile duct ligated, and BDL combined with GH treated.

将大鼠随机分为假手术组、胆总管结扎组和胆总管结扎＋生长激素组3组。

Bcl-2 mRNA was detected by RT-PCR in all cells;（2）After normal rat and bile duct-ligated 14d rat hepatocytes were added 100 μmol/L GCDC and kept for 24 hours, cells were evaluated by FCM and TUNEL. Results:（1）Normal rat hepatocytes did not express Bcl-2 by RT-PCR technique. Bcl-2 was expressed in 7-,14-,21- day BDL rats.

分离对照组及实验组14 d组大鼠的肝细胞行原代培养后，使用100 μmol/L甘氨鹅脱氧胆酸钠作用于两组肝细胞24 h后，用FCM法测定肝细胞的凋亡比率，用TUNEL技术进行肝细胞凋亡的原位检测。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。