查询词典 library binding

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

The open reading frame of cellular nucleic acid-binding protein was cloned from Grass Carp cDNA library.

从草鱼肝肾cDNA文库中克隆到细胞核酸结合蛋白基因CNBP的完整开放阅读框序列。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage. Four positive clones containing chicken H-FABP gene were obtained.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

Binding is the library every year or every other year must be done and when bookbiding journals the most appropriate?

期刊装订是图书馆每年或隔年必须要做的工作，而什么时候装订期刊最为合适呢？

Therefore, we used a computer-based high throughput screening method to screen drug library to identify the possible binding drugs, thus increasing the added value for nsLTPs in drug delivery systems.

我们利用了电脑助药物筛选的技术来进行筛选，试找出可能与此一蛋白结合的药物，并结合萤光光谱的方法加以验证。如此，可进一步提高此一蛋白质的附加价值。

We employed yeast one hybrid system developed by Li in 1993 to screen the human liver cDNA library to fish the proteins binding with the HBV large surface antigen promoter DNA.

目前的研究认为表面抗原大蛋白的表达具有肝特异性，在HBV的嗜肝性及复制循环中扮演着重要角色。

E4Graph1.0a6 - e4Graph is a C++ library and a Tcl binding providing persistent, reliable, cross-platform storage for graph like structures.

e4Graph是一个用来保存如结构图的，由C++库和Tcl捆绑文件提供的稳定的，可靠的，跨平台的库。

I hope that this success is just the first in a long series.

我希望这只是成功的开始。

Three dimensional stresses on the interface of tire and soil, tire deflection and soil sinkage are measured.

测试轮胎——沙土界面上的三向应力、轮胎变形和沙土沉陷；建立考虑剪切应力对沙土承载特性产生影响的车轮牵引性能预测模型；分析车轮作用下土壤的纵向稳定性。