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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

UDZ Series liquid level indicator of flap disassembly fast and convenient, the installation position can be arbitrary choice, free to change the direction of observation, thus instrumented great visibility; UDZ series of cost-effective liquid level was significantly higher than that used multiple sets of glass plate liquid level of cost performance; UDZ series of liquid level indicator, the sender's device, as well as the liquid level with the main transmission medium within the complete separation of the measured structural design, so that level gauge in the job has pressure (up to 320KG), toxic and inflammable conditions, with absolute safety and reliability; UDZ-3 type uses innovative design, improve the sensitivity of the sensor, and its near-zero drift FarEasTone signals and long-term stability so that it has always been at the scene consistent level instructions; sensors and the main body has a certain interval, which can be solved effectively under high-temperature liquids (up to 480 ) remote detection and control; antifrost type UDZ Series Level Gauge, are I plant the patented product (Patent No.: ZL00221022 3), guarantees that the use of cryogenic liquid level gauge is not freezing (such as liquid CO2), the effective guarantee of the level of a normal job.

UDZ系列液位计的翻板指示器拆装快捷方便，安装方位可任意选择，观察方向可随意改动，因而仪表指示的可视度很大；UDZ系列液位计的性能价格比明显高于采用多组玻璃板式液位计的性能价格比；UDZ系列液位计的指示器，发讯器、以及液位变送均与主体内被测介质完全隔离的结构设计，使液位计工作在有压（可达320KG）、有毒和易燃的工况下，具有绝对的安全性和可靠性；UDZ-3型采用了新颖设计，提高了传感器的灵敏度，其远传信号近乎零温漂和长期稳定性使其始终于现场液位指示保持一致；传感器和主体有一定的间隔，因而能有效地解决高温液体（可达480℃）远距离检测和控制；防霜型UDZ系列液位计，是我厂的专利产品（专利号：ZL00221022·3），保证了在低温液体上使用液位计不结冰（如液体CO2），有效的保证了液位计的正常工作。

The flow field construct of meso-β cloud clusters which bring the heavy-hard rainfall in northeast plateau is that the low level cloud cluster is located in the south of converging region, the southern low level influx converged in the cloud cluster and have some affection on the setting up of the rainstorm in the low level. In the middle level, the cloud cluster is lied in near the westerly trough and the southwest airflow ahead of the trough constantly transmits water vapor to the cloud cluster. In the high level, there are a pair of high and low pressure centers around cloud clusters and are obvious divergence airflow, and there is weak ageostrophic high level outflux along pressure force.

产生高原东北部地区大到暴雨天气过程的中—β尺度云团的流场结构特征是：在低层，云团位于辐合区的南部，低层的偏南低空入流在云团内辐合，对大到暴雨的形成有一定的作用；在中层，云团位于西风带低槽附近，槽前的西南气流不断地给云团输送水汽；在高层，云团附近有一对高、低压中心，为明显的辐散气流，沿气压梯度力方向有强度不强的非地转高空出流。

In order to establish the complete dynamic simulation model for the heat recovery steam generator and develop an advanced drum level controller, the methods of probability and time series were used to analyze the field data on drum level sloshing noise. The mean value of the drum level was removed and the field data was de-trended to get the true sloshing noise. Meantime, the AR model was set up for the drum level sloshing noise, and simulation results show that the model realizes matching simulation of the drum level noise signals in the power spectrum. The model can also match the drum level noise successfully in the time domain.

为建立完整的余热锅炉系统动态仿真模型，并基于此模型开发先进水位控制器，采用概率统计和时间序列分析的方法，对现场采集的汽包水位晃荡噪声信号进行了分析，去除汽包水位均值和趋势以获得真实的汽包水位晃荡噪声，建立了相应的汽包水位晃荡噪声仿真AR模型；模型的仿真结果表明：AR模型从功率谱上对信号数据进行了匹配模拟，且使用该模型作为噪声模块在时域上对汽包水位晃荡噪声信号进行了成功匹配。

Pleasing to the eye from ground level up this detail, taken in the back garden, is a testament to integrating planting materials for texture/color, scale and balance.

令人高兴的是从地关注这个详细程度，在后花园的话，是为了整合种植纹理材料/颜色，规模和平衡的证明。

Firstly, according to study dynamic changes of the elements structures in succession about natural and artificial forest, then summarized regular patterns;at the same time.based on the study of Coupling Relationship between structure and function of water conservation forest established the ideal structures from the stand level and the watershed level. The ideal structures from the stand level: uneven-aged, double layer and mixed structure, and trend to natural forest. The watershed level followed the same principle, that is double layer patches, uneven-aged forest well-distributed and to build up different forest patches in watershed.

首先，经过对北京山区水源林天然林和人工林结构要素的演替动态研究，提出其发展规律，同时依据水源林结构与功能耦合关系研究，从林分层次和流域层次上分别构建了水源林的理想结构，其中，林分层次上水源林的理想结构为：异龄、复层、混交的结构状态，林分结构趋于天然林结构的特征；在流域层次上水源林的理想结构为也应形成&复层&、&异龄&、&混交&的结构，即：在流域各水源林斑块间形成林冠层复层、斑块内部乔木、灌木、草本层的复层；不同水源林年龄面积上的均匀分布；形成不同水源林斑块间的镶嵌分布。

Design on the principle that security , economy are rational , esthetic , fix up the selection of structure and structural level of every floor, assign , turn into with PK software AutoCAD revise the part behind the structural level well; Adopt stair and floor calculating and designing a teacher to appoint by hand, and calculate the appointed floor with PKPM software, compare with the floor that calculates by hand, fetch the drawing construction drawing of the head of the two; Calculate by hand the frame which the teacher appoints loads, set up good PKPM and calculate the procedure model of the frame , calculate frame internal force and output file result with PKPM , calculate , get PKPM structure mix muscle picture turn into AutoCAD carry on part punish; Build up pieces of internal force association from frame that PKPM obtain , choose three group unfavorable association design the materials as the foundation most, carry on the design basis of the craft, and draw and obtain the basic detail drawing and match the muscle with AutoCAD.

本毕业设计以指导老师发放的《港市公安局巡警支队办公楼》建筑施工图纸为原始资料，进行结构设计和施工组织设计，设计的主要依据为现行的建筑行业规范以及指导老师的指导思想。设计以安全、经济合理、美观为原则，进行结构选型和各楼层结构平面布置，用PK 软件布置好结构平面后再转到AutoCAD上进行局部修改；采用手工计算设计老师指定的楼梯和楼板，并用PKPM 软件计算指定楼板，与手工计算的楼板进行比较，取二者之长绘制成施工图；手工计算老师指定的框架荷载，建立好PKPM计算框架程序模型，用PKPM计算框架内力并输出文件结果，把PKPM计算得到结构配筋图转到AutoCAD进行局部处理；从PKPM得出的框架构件内力组合，选择三组最不利的组合作为基础设计资料，进行手工设计基础，并用AutoCAD绘制得出基础详图及配筋。

Design on the principle that security , economy are rational , esthetic , fix up the selection of structure and structural level of every floor, assign , turn into with PK software AutoCAD revise the part behind the structural level well; Adopt stair and floor calculating and designing a teacher to appoint by hand, and calculate the appointed floor with PKPM software, compare with the floor that calculates by hand, fetch the drawing construction drawing of the head of the two; Calculate by hand the frame which the teacher appoints loads, set up good PKPM and calculate the procedure model of the frame , calculate frame internal force and output file result with PKPM , calculate , get PKPM structure mix muscle picture turn into AutoCAD carry on part punish; Build up pieces of internal force association from frame that PKPM obtain , choose three group unfavorable association design the materials as the foundation most, carry on the design basis of the craft, and draw and obtain the basic detail drawing and match the muscle with AutoCAD.

建筑毕业设计计算书74页、CAD图纸、施工组织设计全套摘要：本毕业设计以指导老师发放的《港市公安局巡警支队办公楼》建筑施工图纸为原始资料，进行结构设计和施工组织设计，设计的主要依据为现行的建筑行业规范以及指导老师的指导思想。设计以安全、经济合理、美观为原则，进行结构选型和各楼层结构平面布置，用PK 软件布置好结构平面后再转到AutoCAD上进行局部修改；采用手工计算设计老师指定的楼梯和楼板，并用PKPM 软件计算指定楼板，与手工计算的楼板进行比较，取二者之长绘制成施工图；手工计算老师指定的框架荷载，建立好PKPM计算框架程序模型，用PKPM计算框架内力并输出文件结果，把PKPM计算得到结构配筋图转到AutoCAD进行局部处理；从PKPM得出的框架构件内力组合，选择三组最不利的组合作为基础设计资料，进行手工设计基础，并用AutoCAD绘制得出基础详图及配筋。

Design on the principle that security , economy are rational , esthetic , fix up the selection of structure and structural level of every floor, assign , turn into with PK software AutoCAD revise the part behind the structural level well; Adopt stair and floor calculating and designing a teacher to appoint by hand, and calculate the appointed floor with PKPM software, compare with the floor that calculates by hand, fetch the drawing construction drawing of the head of the two; Calculate by hand the frame which the teacher appoints loads, set up good PKPM and calculate the procedure model of the frame , calculate frame internal force and output file result with PKPM , calculate , get PKPM structure mix muscle picture turn into AutoCAD carry on part punish; Build up pieces of internal force association from frame that PKPM obtain , choose three group unfavorable association design the materials as the foundation most, carry on the design basis of the craft, and draw and obtain the basic detail drawing and match the muscle with AutoCAD.

土木工程毕业设计 6层办公楼计算书+图纸+施工设计全套）摘要：本毕业设计以指导老师发放的《港市公安局巡警支队办公楼》建筑施工图纸为原始资料，进行结构设计和施工组织设计，设计的主要依据为现行的建筑行业规范以及指导老师的指导思想。设计以安全、经济合理、美观为原则，进行结构选型和各楼层结构平面布置，用PK 软件布置好结构平面后再转到AutoCAD上进行局部修改；采用手工计算设计老师指定的楼梯和楼板，并用PKPM 软件计算指定楼板，与手工计算的楼板进行比较，取二者之长绘制成施工图；手工计算老师指定的框架荷载，建立好PKPM计算框架程序模型，用PKPM计算框架内力并输出文件结果，把PKPM计算得到结构配筋图转到AutoCAD进行局部处理；从PKPM得出的框架构件内力组合，选择三组最不利的组合作为基础设计资料，进行手工设计基础，并用AutoCAD绘制得出基础详图及配筋。

Design on the principle that security , economy are rational , esthetic , fix up the selection of structure and structural level of every floor, assign , turn into with PK software AutoCAD revise the part behind the structural level well; Adopt stair and floor calculating and designing a teacher to appoint by hand, and calculate the appointed floor with PKPM software, compare with the floor that calculates by hand, fetch the drawing construction drawing of the head of the two; Calculate by hand the frame which the teacher appoints loads, set up good PKPM and calculate the procedure model of the frame , calculate frame internal force and output file result with PKPM , calculate , get PKPM structure mix muscle picture turn into AutoCAD carry on part punish; Build up pieces of internal force association from frame that PKPM obtain , choose three group unfavorable association design the materials as the foundation most, carry on the design basis of the craft, and draw and obtain the basic detail drawing and match the muscle with AutoCAD.

设计以安全、经济合理、美观为原则，进行结构选型和各楼层结构平面布置，用PK软件布置好结构平面后再转到AutoCAD上进行局部修改；采用手工计算设计老师指定的楼梯和楼板，并用PKPM软件计算指定楼板，与手工计算的楼板进行比较，取二者之长绘制成施工图；手工计算老师指定的框架荷载，建立好PKPM计算框架程序模型，用PKPM计算框架内力并输出文件结果，把PKPM计算得到结构配筋图转到AutoCAD进行局部处理；从PKPM得出的框架构件内力组合，选择三组最不利的组合作为基础设计资料，进行手工设计基础，并用AutoCAD绘制得出基础详图及配筋。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。