查询词典 laser induced fluorescence

To establish new methods for highly sensitive time-resolved fluorescence bioassay, several new lanthanide fluorescence complexes were synthesized and used for the preparations of nanometer-sized lanthanide fluorescence biolabels. Novel lanthanide fluorescence nano-biolabels are uniform (25-55 nm in diameters),strongly fluorescent with long fluorescence lifetime, highly photostable and biocompatible.

本项目以时间分辨荧光生物分析新方法的建立与应用为研究目的，在合成出几种稀土荧光配合物的基础上，建立了几种新型强荧光性纳米稀土荧光生物标记材料的可控制备方法，生物标记方法及其在时间分辨荧光免疫分析中的应用方法。

To establish new methods for highly sensitive time-resolved fluorescence bioassay, several new lanthanide fluorescence complexes were synthesized and used for the preparations of nanometer-sized lanthanide fluorescence biolabels. Novel lanthanide fluorescence nano-biolabels are uniform (25-55 nm in diameters),strongly fluorescent with long fluorescence lifetime, highly photostable and biocompatible.

中文摘要：本项目以时间分辨荧光生物分析新方法的建立与应用为研究目的，在合成出几种稀土荧光配合物的基础上，建立了几种新型强荧光性纳米稀土荧光生物标记材料的可控制备方法，生物标记方法及其在时间分辨荧光免疫分析中的应用方法。

And a fluorescence molecule beacon probe is designed between upstream and downstream priers, 5' end of probe can mark fluorescence reporter group FAM, and the 3' end markes fluorescence quenching group DABCYL, and 5' end and 3' end of the probe have 6 bases which can be reflected and complemented to make fluorescence molecule beacon probe do not give out fluorescence signal in free state.

在上下游引物中间设计一条荧光分子信标探针，探针的5'端标记荧光报告基团FAM，3'端标记荧光淬灭基团DABCYL，探针的5'端和3'端有6个碱基能反折互补，使荧光分子信标探针在游离状态下不发出荧光信号。

The mechanical property testing shows that the seamless PLLA tubes perform excellent radial strength,which indicates that the mechanical properties of biodegradable stents made of these tubes was supposed to be excellent.PLLA and its composites were first used in preparing seamless tubes as antecedency of laser-cut biodegradable coronary stents in china.Finite element analyses of the biodegradable coronary stent model indicate the explorative design basically meets the requirement of reasonable and applicable stents.There is not such kind of research yet in domestic.Optimum laser-cuttting parameters of CO2 laser was cauculated based on experiential formula,within it,biodegradable coronary stents,especially those with complex structure would be manufacutured from seamless tubes quickly by laser microprocessing with high consistency to the design model.The processing efficiency of the CO2 laser demonstrated in experiential formula by us will play an important guiding role in manufacturing biodegradable stents by laser-processing.Our stents that ascribed to laser-cutted,slotted tube stent are proved to be biodegradable, self-expandable and good biocompatible,basically reach the international level on the aspest of manufacturing procedures and design.

研究结果表明：壳聚糖改性材料综合性能优于纯PLLA，更适合于作为可降解冠脉支架的原材料；自主设计的探索性自膨胀管状闭环型可降解冠脉支架的性能符合冠脉设计要求，设计、验证生物可降解冠脉支架模型的研究在国内还未见报道；制备的改性PLLA无缝管的力学性能优越，可通过激光切割获得冠脉支架，这是国内首次采用聚乳酸材料来制备通过激光加工出可降解冠脉支架的无缝管；二氧化碳激光器加工精度能达到加工要求，加工出来的血管支架名义设计与实测值误差小，实现了冠脉支架、尤其是带有复杂外观设计冠脉支架的快速化、规范化加工，对二氧化碳激光器加工聚乳酸材料的效果研究在国内外属于首创，获得的经验公式对日后的深入研究有重要的指导意义；制备的支架为激光切割管状闭环型冠脉支架，支架外观设计、支架加工手段、支架的性能都基本接近国际水平，支架样品符合设计的理念，可以实现自膨胀功能。

Our main work is as follows: the stable laser mode theory in uniform plasmas is extended to non-uniform plasma cases; the discussion of the sufficient and necessary conditions for laser self-focusing in uniform plasmas is developed to non-uniform plasma cases; a quantitative approach to judge the impact of different plasma density distribution on laser self-focusing is proposed; the evolution properties of asymmetric laser filed in non-uniform and uniform plasmas are discussed and mechanisms that symmetric channel-like plasmas can confine the laser centroid around the plasma channel axes and symmetrize initially asymmetric laser beams are proposed; the formation of solitary standing wave in moderate plasma density is studied by PIC simulations and can be explained by stimulated Raman scattering of laser beams in plasmas.

主要工作为：把均匀等离子体中激光的稳定模理论推广到横向非均匀等离子体情形；把均匀等离子体中激光自聚焦的充分和必要条件的讨论推广到横向非均匀等离子体情形；给出横向密度分布不同的等离子体影响激光自聚焦的定量判据。讨论了初始不对称的激光场在横向非均匀及均匀等离子体中的演化特性，并提出了具有对称腔道结构的等离子体箍缩激光质心和对称化初始不对称激光场的物理机制。用粒子模拟讨论了激光场在等离子体中形成孤立驻波的过程，认为前向受激拉曼散射是发生此现象的可能机制。

In the second chapter the experiment technique and approaches are discussed,including laser induce fluorescence spectroscopy which the comprehensive adopted for liner laser spectroscopic study,and high resolution two-photon laser spectroscopy without Doppler broadening which belonged to nonlinear laser spectroscopic ,and the theory about the fluorescence rate equation .

第二章实验技术和研究方法中，讨论了在线性激光光谱中最为常用的激光感应荧光光谱、非线性激光光谱学可使谱线的多普勒宽度得以消除无多普勒双光子吸收光谱学和碰撞分析中使用的荧光的速率方程理论。

To investigate the fluorescence dynamic change of protoporphyrin IX in bladder carcinoma cell induced by 5-ALA and the influence of 5-ALA concentration and pH on fluorescence of PpIX. 2. To discuss the biodistribution and express of PpIX in bladder wall of different lesions by method of instilling of 5-ALA into bladder cavity. 3. To discuss the clinical value of the fluorescence diagnosis in early detection of bladder cancer and the radicality of bladder tumor resection under fluorescence cystoscopy.

本研究目的：1了解5-ALA诱导膀胱癌细胞内生原卟啉IX荧光随时间的动力学变化及5-ALA的浓度、pH值对产生PpIX荧光的影响。2通过膀胱灌注5-ALA探讨其所诱导内生PpIX荧光在膀胱上皮中分布及不同病变表达上的差异。3探讨5-ALA诱导荧光膀胱镜检对发现早期膀胱癌的临床应用价值及在荧光膀胱镜下膀胱肿瘤电切治疗的彻底性。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

Hongsheng specializing in the production of decorative materials such as: special silver, black, silver pieces, small horizontal line, gold line, gold pieces, 1 / 4 gold box, gold line spectrum, Silverstrand spectrum, gray gold stripes, black gold stripes , pearl white, pearl black, pearl light gray, dark gray pearl, red laser wire, white wire laser, laser transparent silk, gray silk laser, laser dark gray silk, silk blue laser, red wood, silver gray in stripes, blue silver box and so on.

鸿盛专业生产装饰材料如：银特，黑皮，银碎，小横线，金横线，金碎，1/4金方格，金线谱，银线谱，灰金条纹，黑金条纹，珠光白，珠光黑，珠光浅灰，珠光深灰，红镭射丝，白镭射丝，透明镭射丝，灰镭射丝，深灰镭射丝，兰镭射丝，红木纹，银灰中条纹，兰银方块等等。

Poly decade refining production applications rich practical experience, independent research and a series of laser processing equipment, further re-processing base in actual production Strict testing, continuous improvement, and ultimately the formation of "laser" brand laser cutting board production equipment Products: laser knife mold cutting machine, laser plotter, laser proofer, computer automatically machetes, and other cutting board production related equipment.

聚十年之生产應用所提煉的丰富实践经验，自主研制出系列激光加工设备，再经加工基地的严格实际生产檢驗，不断完善，最终形成"嘉洛激光"品牌的激光模切板制作设备系列产品：激光刀模切割机、激光绘图机、激光打样机、電腦自动弯刀机及其它模切板制作相关设备。

Some questions asked by the adjudicators in one English Speaking Contest still run upon me very often.

我参加了一场以"我心中的奥运会"为主题的英语演讲竞赛，其间评判提的几个问题时时萦绕我的心头。

He called InTERActive our most profitable release of the year.

他呼吁互动的我们的最有利可图的释放一年。