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The ecdysis index of controlled larvae was 3.323 by 30d, while 2.933-3.038 for the treated. The transgenic Betula platyphylla could reduce the feeding of larvae. With the increase of the time of feeding, the difference of food taken between the controlled and treated became larger. The food of controlled larvae taken was 0.105293-0.13750 grams than that of the treated by 3 d. and 0.529094-0.64103 grams by 21d.

转基因植株能够降低幼虫的食叶量,3天时对照幼虫的食叶量比处理幼虫的食叶量多0.105293~0.137504克;21天时对照幼虫食叶量比处理幼虫食叶量多0.529094~0.64103克,随取食时间的延长,对照幼虫的食叶量与处理幼虫的食叶量差别更大,转基因植株的抑制作用更明显。

Change 6: Mutant Larvae aren't so Mutant After All: Mutant Larvae definitely got kicked in the jaw on this one. They're almost completely useless now. You choose: spend 300 on a new hatchery and have it done for you, or click every ten seconds on your hatchery to produce extra larvae. I'll take the first one, thank you very much. What was once possibly the coolest mechanic the Zerg has now disappeared like Jimmy Hoffa. Maybe we'll find it in the Giants stadium too. Perhaps this nerf was necessary because of the ubiquitous nature of the Queen. In that case… nope, still a bad idea.

变化6:变异幼虫几乎完全无用,你可以选择:使用300水晶来建造一个新的基地为你工作,或是每10秒点击你的基地来或则一个额外的幼虫,而我会选择第一个,这个可能是虫族最酷的一个机制现在也消失了,也许这个削弱在女王可以重复建造后是必须的,但是这仍然是个坏主意,我对这个改变评分C-。

The larvae in the control group metamorphosed at day 20, and the metamorphosis rate increased gradually. The larvae in group 1 metamorphosed at day 30, but those in group 2 did not metamorphose throughout the experiment, which showed that the larvae did not have enough nutrition for metamorphosis.

对照组幼虫在第20天时开始变态,随后变态率逐渐升高,试验一组幼虫在第30天时开始变态,而试验二组幼虫在试验期间一直没有变态,表明该组幼虫的营养状况不足以支持其变态附着。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位:山西医科大学寄生虫教研室,太原 030001;化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽,然后经过研磨、离心和层析等过程,将家蝇幼虫抗菌肽进行分离纯化,采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后,筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽,而且不止一种,但其效果有所不同。

After Cryopreservation of embryo and larvae of pacific oyster Crassostrea gigas, the results indicate the survival rate of trochophore larvae is 61.1% and significantly better than that of other stage embryo or larvae(P.05).The effect of high sucrose density is better than that of low sucrose density with the same DMSO density.

对太平洋牡蛎的胚胎和幼虫的超低温冷冻保存发现,担轮幼虫的解冻复活率最高,达61.1%(3.0M DMSO),显著高于其他发育阶段幼虫的保存结果(P<O.05);牡蛎担轮幼虫的保存结果显示,在相同DMSO浓度条件下,高蔗糖浓度组保存效果好于低蔗糖浓度组,但差异不显著(P>0.05)。

The studies on the nymphs or larvae of 18 species belonging to Orthoptera, Mandotea, Homoptera, Hemiptera, Coleptera, Diptera and Hymenoptera indicate that the larvae of Argridae have the function of ""lungs"", but the nymphs or larvae of other orders may not have ""lungs"".

如果&肺&功能扩大到换气性气管,那么,膜翅目中的蜜蜂科、双翅目中的蝇科幼虫及半翅目、鞘翅目等中存在换气性气管部分,也应具有&肺&功能。

In the artificial rearing larval honeybee method, 1-day-old larvae were reared in laboratory and 0.1 - 100 ppm pyriproxyfen was added in larval food. A dose respondent effect was found in the larval development of the treated larvae. Results showed that 0.1 ppm pyriproxyfen reduced the eclosion rate of the reared larvae conspicuously, and 1 ppm pyriproxyfen reduced that 84.6% of eclosion honeybee appeared the condition of deformed wings. The 10 ppm pyriproxyfen treatment caused 53.8% melanic death in the pupal stage, and no honeybees succeed in eclosion.

在室内饲养幼虫方面,分别添加0.1-100 ppm百利普芬於幼虫食物中,结果显示0.1 ppm百利普芬即会显著降低饲养幼虫的羽化率,而且添加1 ppm百利普芬会有84.6%羽化蜜蜂出现残翅现象;添加10 ppm百利普芬则会造成53.8%蜜蜂在蛹期黑化而死亡,而且无蜜蜂羽化成功;100 ppm百利普芬则有50%蜜蜂在幼虫期死亡,50%蜜蜂在蛹期死亡,亦无蜜蜂成功羽化。

Our previous studies have shown that ubiquitous overexpression of HmgD caused hemocytes proliferation and the formation of melanotic tumors in the Drosophila larvae. To clarify the mechanism of melanotic tumors appearance,we also generated RT-PCR analysis to test the transcriptional changes of PPAE and Spn27A,which encode the key factors in the phenoloxidasecascade pathway from larvae samples of mid-third instar,late-third instar and prior to pupation relative to wild type flies.Our result showed that in the HmgD overexpression mid-third instar larvae,the expression level.of Spn27A was lower than wild type,otherwise the expression level of PPAE was higher than wild type.It suggests that overexpression of HmgD affects the PO cascade pathway.

本研究组前期工作发现,过量表达HmgD引起果蝇幼虫体内血细胞增生,并出现黑色素瘤,为探讨HmgD过量表达导致了血细胞增生即形成黑色素瘤的分子机制,本研究分别提取三龄中期、后期和相对于野生型化蛹前时刻的幼虫的RNA,首先检测了编码酚氧化酶级联反应途径中的两个关键基因PPAE,Spn27A在突变体中的转录发生变化,结果发现,Spn27A在三龄中期的表达量低于野生型,而PPAE的表达高于野生型,表明过量表达HmgD影响了酚氧化酶级联反应途径,低水平的Spn27A不足以抑制PPAE的活性,活化的PPAE催化了PPO的活性,使其转变成有活性的PO,从而促进黑色素的生成,使果蝇体内产生黑色素瘤。

Then the number of larvae in the stem increased sharply for a great number of newly-hatched larvae appeared. Though some larvae transferred from stem to root before overwintering, about 43% of the larvae overwintered in tree stems at the height between 40 and 120 cm in the end of September till after the overwintering the next year.

之后,由于初孵幼虫的大量出现,使干部幼虫数量急剧增加,越冬前,虽然有部分转移至根部危害,但到9月末仍有近43%的幼虫停留在树干40~120cm处越冬,直到次年越冬结束。

The results showed that the antlions fed on M. domestica larvae obtained significantly higher body weight, pupal weight, relative growth rate and pupation percentage, and significantly shortened larval duration when compared with the antlions fed on S. litura and S. exigu larvae. When feeding on C. cephalonica larvae, antlions had similar body weight, pupal weight and pupation percentage, but significantly decreased relative growth rate and prolonged larval duration, than those fed on M. domestica larvae.

结果显示,用家蝇幼虫饲养的蚁狮,其体重增长、相对生长率、化蛹率、蛹重均显著高于用斜纹夜蛾和甜菜夜蛾幼虫饲养的蚁狮,而幼虫历期则比斜纹夜蛾和甜菜夜蛾幼虫饲养的短;用米蛾幼虫饲养,虽然蚁狮体重增长、化蛹率和蛹重与用家蝇幼虫饲养的差异不显著,但其相对生长率却显著低于用家蝇幼虫饲养的蚁狮,幼虫历期也比用家蝇幼虫饲养的明显延长。

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