查询词典 laddering

The data indicated that while there was an extended G2/M arrest of the irradiated cells with a maximum 80% accumulation at 36hrs after 10Gy irradiation, there was no evidence of DNA laddering detectable up to 4 days PI.

结果显示，细胞经10格雷加马辐射照射后，其细胞周期会延滞在G2/M期，且在照射后36小时达到最大量，约有80%的细胞停留在G2/M期，同时在照射后四天内皆没有量测到DNA段裂的现象。

Trypan blue exclusion assay and cell growth curve were used to observe proliferation. Fluctuation of CD11b, reactive oxygen species and NF-кB were detected using flow cytometry. Cell apoptosis was observed using DNA laddering assay.

选择NB4细胞，加入ATRA和不同剂量的As2O3，观察细胞生长曲线，流式细胞仪检测CD11b、ROS、NF-кB的变化，DNA片段梯度观察细胞凋亡。

We also found that apoptosis was induced by terfenadine in human cancer cell by DNA laddering formation. In this study,we demonstrated that p21、p27、BAX and BAD protein were induced,in contrast,Bcl-2 protein was down regulated by treated with terfenadine.

经过琼胶电泳分析Terfenadine会使人类癌细胞产生DNA ladder的特徵，表示细胞发生凋亡，并随著剂量的增加我们发现：p21、p27、Bax、Bad表现量上升，而PARP有被切断的片段产生，Bcl-2、Caspase-3减少，而且p27有被切断的情形产生。

High proportion of cells was in Sub-G1 phase of cell cycle and the cells could not grow in soft agar to form colonies. On the contrary, the three breast cancer cell lines, Bcap-37, MCF-7 and MDA-MB-231, are anoikis resistant, as indicated by DNA laddering and FCM assays.

而Bcap-37、MCF-7、MDA-MB-231三种乳腺癌细胞则具有抗脱落凋亡的特性，分别与它们各自贴壁培养的细胞比较，胞浆DNA琼脂糖凝胶电泳表现无明显差异，FCM所检测到的凋亡峰也无明显区别。

Treatment with dopamine and low dose bromocriptine resulted in cell death accompanied with the DNA laddering and activation of caspase-3 only in GH3D2L and GH3D2S cells, which were blunted by haloperidol, showing that dopamine-induced cell death in these cells is mediated by dopamine D2 receptors.

经多巴胺和低浓度溴隐亭处理后仅在GH3D2L和GH3D2S两种细胞中出现细胞死亡伴DNA阶梯状断裂和半胱天冬酶3（Caspase-3）的激活，而这些效应也被氟哌啶醇所抑制，说明多巴胺所致的细胞凋亡与多巴胺D2受体激活有关。

This research employs means-end chain model to explore the formation of consumption value by in-depth interviewing eighty consumers of hand-touch products with laddering technique, and hence to confirm the critical benefits of hand-touch products according to the benefit evaluation model.

为此，本研究运用方法目的链结模式，以阶梯法深度访谈80位手感产品消费者，探讨其消费价值的形成历程，并依据Pieters， Baumgartner and Allen (1995)所提的产品利益评价方式，确认手感产品的关键利益。

A 30 min exposure to peroxynitrite (10〓μmol/L) followed by incubation in fresh medium induced apoptosis in cultured cerebellar granule cells, which was confirmed by DNA laddering, chromatin condensation, nuclei fragmentation, and the formation of apoptotic bodies.

原代小脑颗粒细胞经10μmol/L过氧化亚硝基处理30min，并继续培养一定时间后逐渐发生细胞凋亡，主要表现为DNA链断裂、染色质凝缩、核破裂、形成凋亡小体。

Simvastatin can induce apoptosis in 15% FCS activated proliferating mesangial cell, as evidenced by typical morphological changes and DNA laddering.

通过相差显微镜、Gimsa染色、电镜等形态学观察和DNA梯形带证明辛伐他汀(1uM、5uM、25uM)可以诱导在15％FCS条件下，活跃增殖的系膜细胞发生凋亡。

We may well explain why ricin-induced apoptosis showed no typical DNA ladder. The presence of laddering in DNA gels implies an apoptosis process, but its absence does not rule out the apoptosis. The ricin 0.05μmol〓 had no effect on the 〓 phase of cell cycle, but induced 〓 arrest. The ricin may affect the balance of synthesis and destruction of proteins that controlling the cell cycle.

检测到DNA梯度表明细胞发生了细胞凋亡，未检测到DNA梯度不能否认凋亡的存在。0.05μmol.L〓的蓖麻毒素对HeLa细胞周期的G〓/G〓期无影响，而诱导了G〓/M期阻滞，蓖麻毒素可能引起一些控制细胞周期蛋白的合成与降解发生紊乱。

After 4 days of serum deprivation, CPR-293 cells exhibited substantial nuclear condensation and fragmentation DNA laddering but HO-1/CPR-293 cells was not apparent. Thus HO-1 expression is sufficient to protect cells from serum deprivation-induced apoptosis. In the experiment, we used primary cultures of from HO-1 fibroblasts and their wild-type littermates fibroblasts CPR-293 cell and HO!

2生理加压诱导细胞凋亡我们用稳定的CPR细胞株和HO1和CPR在一起的细胞株实验，当浆液丢失4天后，CPR-293细胞表现出有实质的核浓缩和DNA梯状条带的形成，而HO1/CPR-293细胞则没有，显示HO1是充分保护细胞，避免在浆液丢失情况下而诱导的细胞凋亡。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。