查询词典 labelled

Results: HRP labelled cells,5-HT labelled cells and 5-HT-HRP dou-bled cells were found in nucleus raphe dorsalis .

结果：在中缝背核内可见到HRP标记细胞、5-HT阳性反应细胞和5-HT-HRP双标细胞。

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法，该方法包括：使用一套核苷酸对所说的样品进行扩增反应，其中至少一个核苷酸是被荧光标记的，在探针与目标序列杂交的条件下使扩增产物与探针混合，所说的探针包括一种活性分子，该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量，及检测样品的荧光。

After injection of HRP into the cervical trunk of the vagus nerve in the goat, a large number of labelled cells was found in the nodose and jugular ganglia ipsilaterally,however, the labelled cells of the former were much more and denser than that of the later.

为了探讨山羊迷走神经感觉纤维的来源，本文将HRP注入颈迷走神经干后，在结状节出现大量密集的标记细胞，颈静脉节中也有较多的细胞被标记，但其密度和数量远不如结状节。

The effects of the total alkaloids isolated from the root of Rauwolfia verticillata from Hainandao on the circulatory and organ plasma and cell volumes were studied by using I~(131)-labelled serum protein and P~(32)-labelled red blood cells.

用I~(131)标記血清蛋白及P~(32)标記紅血球研究海南島蘿芙木根硷对循环及器官血浆及血球容量的影响，发見：正常或腎型高血压大白鼠靜脉注射此硷5毫克/公斤，5分鉀后，循环血浆及血球量均无明显影响。

SERS labelled immunogold was then used as probe to detect the interaction between the Goat anti-rat IgG and silver substrate (which we will use it as the substrate for solid antibody). It is found from experiment that SERS labelled immunogold can be non-specifically adsorbed onto silver surface uniformly because of the strong interaction between the protein molecule and silver.

由于我们设想以银片为免疫检测中固相抗体的基底，所以论文中首先以SERS标记的免疫金溶胶为探针，讨论了抗体与银表面间的作用，实验中发现，SERS标记的免疫金溶胶可以通过抗体与银之间的作用较好的自组装于银表面，同时，银表面上可以测到很强的苯硫酚的SERS信号。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

After HRP application to the rostral area (n=10), quite a number of labelled cells were distributed at the ventrolateral part of the nucleus of trapezoid body, the lateroventral periolivary nucleus and the retrotrapezoid nucleus. After HRP application to the caudal area (n=10), HRP labelled cells were mainly located at the lateral reticular nucleus and the ventral part of at the lateral paragigantocellular nucleus LPGi. After HRP application to the intermediate area (n=6), only 8HRP labelled cells were observed at the in 6rats. After HRP application to the control area (n=4), only 6HRP labelled cells were found at the rostral ventrolateral reticular nucleus.

大鼠延髓腹外侧表面的头端化学感受区表面敷贴辣根过氧化物酶后，在延髓斜方体核、外周橄榄腹外侧核和斜方体后核均有HRP标记细胞存在；于尾端化学感受区表面敷贴HRP后，在延髓的巨细胞旁外侧核、外侧网状核和舌下神经核有HRP标记细胞存在；于中间区表面敷贴HRP后，在6只大鼠的延髓内部区域仅观察到8个HRP标记细胞；于对照区敷贴HRP后，在4只大鼠的延髓内部区域仅观察到6个HRP标记细胞，散在地分布在头端腹外侧网状核。

The 33P, 32P double label tracing technique was carried out to study the effective fertilization of phosphorus for citrus plantation in mountain area of northern Taiwan. 33P labelled orthophosphoric acid was applied in a standard position with respect to the tree and 32P labelled orthophosphoric acid at a location and rate corresponding with the treatments The results showed that the variability o1 the 33P/32P ratios (49%) was much lower than that of single 32P activity counts (72%).

应用磷－33，磷－32重复标识法，研究本省北部山区柑园有效施肥问题；其法将磷－33标识正磷酸，依标准法施用於根羣密叶区域，又以磷－32标识正磷酸以不同施用量，处理於不同深度，结果显示以33P/32P比率所得试全变异(49%)达较单独测算32P放射活性者(72%)为小。

The weakly or strongly stained CaBP-LI/CB-Audouble-labelled cells gradually increased caudalward.In the middle rostrocaudal third of the red nucleus,the double-labelled neurons mainly occupied the ventromedial portions of the red nucleus.

两种神经元在红核具有特殊的分布方式，头侧水平CB-Au标认的红核-脊髓投射神经元均呈CaBP-LI阴性，向尾侧CaBP-LI阳性神经元数目逐渐增多。

The weakly or strongly stained CaBP-LI/CB-Audouble-labelled cells gradually increased caudalward.In the middle rostrocaudal third of the red nucleus,the double-labelled neurons mainly occupied the ventromedial portions of the red nucleus.

两种成年人神经元在红核具有特殊的分布方式，头侧水平CB-Au标认的红核-脊髓投射成年人神经元均呈CaBP-LI阴性，向尾侧CaBP-LI阳性成年人神经元数目逐渐增多。

It goes back to what I told you...

现在回到我告诉过你的。。。

With a supporter in Mr Charest and an admirer in Mr Dumont, Mr Harper may be encouraged to call an election himself.

由于在沙雷那边有个支持者，杜蒙那边有个崇拜者，鼓励哈珀为自己举行一次选举。