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From the 105 isolates, we choosed 20 isolates that had good conidia and strong pathog enic ability.

从供试的105个菌株中,筛选了20个产孢量好、致病力强的菌株进行了继代培养。

The frequency distributions of EC50 values of the 65 isolates were normally distributed for both conidial germination and mycelial growth. Thus, the mean EC50 values 0.042 6 ± 0.030 4 and 0.025 0±0. 012 9 μg/mL of the 65 isolates could be used as the sensitivity baselines for conidial germination and mycelial growth of P. digitatum to azoxystrobin, respectively.

敏感性频次分析结果表明,该65个菌株孢子萌发和菌丝生长对嘧菌酯的敏感性频率分布均符合正态分布,其EC50平均值0.042 6±0.030 4μg/mL和0.025 0±0.012 9μg/mL可分别作为柑橘绿霉病菌孢子萌发和菌丝生长对嘧菌酯的敏感基线。

None samples of the eggs are salmonella-positive. In the event of serotyping, Salmonella Enteritidis was the most common serotype accounting for 91.6 %(329/359)of Salmonella isolates. And 35.5 %(128/359)of the isolates which were all Salmonella Enteritidis were not antimicrobial resistant .

血清分型结果以Salmonella Enteritidis血清型为最多,占总数的91.6 %(329/359),所有菌株的35.5 %(128/359)不具抗药性,但全都是Salmonella Enteritidis血清型,其余菌株主要是对Colistin及Tetracycline具有较高的抗药性。

In our study, 3 genotypies have been respectively demonstrated in UL139 and UL149 for the first time, and the corelations between certain genotypical structure and certain diseases were also proposed; The existence of the 3 genotypes of UL144 was foremost verified in isolates from congenital infants; Furthermore, the UL140, UL141 and UL145 genes were observed to be greatly discrepant to those had been described previously: comparing with Toledo, 231 nt are inserted in UL140, 2 more ORFs are obtained in UL141, and the UL145 ORF moves upstream by 90 nt. Except UL144, the sequences of 18 else genes in clinical isolates were submitted for the first time, and 479 sequences were assigned by GeneBank in total. Relevantly, 9 papers were published.

我们在学术界首次证实了HCMV UL139、UL149两个基因在临床分离株中分别存在三种基因型,发现了其中某个基因型的基因结构与特定来源的分离株存在一定的对应关系;首次在先天感染分离株中验证了UL144三种基因型的存在;首次证实了UL140、UL141和UL145等基因与原先认识的基因结构不同,其中UL140基因较Toledo株增加了231个碱基、UL141产生2个新的基因编码区、UL145 ORF较Toledo株前移90个碱基;首次或最先提交了除UL144基因外其余18个基因的临床分离株序列,本项目组共有479个HCMV相关基因序列被GenBank收录;发表研究论文9篇。

The genus specificity was validated with 47 strains including wild type isolates. Preliminary screening of soil samples with Gordonia-specific primers was successful in the rapid detection of the presence of 36 Gordonia wild-type isolates.

经多序列分析比对软体计算并比较gyrB基因序列,可知Gordonia菌属与7种邻近放线菌的gyrB基因序列相似度介於72.8-82.1%,而在16S rDNA序列相似度则介於90.0-95.4%,gyrB基因可明显区分Gordonia菌属与其他属之放线菌。

The most common isolates were α-hemolytic streptococcus, Hemophilus influenzae, Streptococcus pneumonia, coagulase-negative Staphylococcus, and Staphylococcus aureus. Anaerobes were recovered from 7.9% of all isolates.

最常见的菌种依序是α-hemolytic streptococcus,Hemophilus influenzae,Streptococcus pneumonia,coagulase-negative Staphylococcus以及Staphylococcus aureus,厌氧菌则占所有菌株的7.9%。

Among 558 isolates, 65 (11.6 ~)isolates produced hyperproduction AmpC β-lactamases, 135 (24.2 ~) iso- lates produced ESBLs,and 28(5.0%)produced both enzymes.

对阴沟肠杆菌进行耐药性监测及去阻遏持续高产AmpC酶和ESBLs检测,能较好了解该地区细菌耐药的现状,为当地临床医生抗生素的使用提供合理建议。

Results:67(30.45%)strains of H.pylori were isolated from gastric mucosa;The positive of H.pylori rate was 20.15%(27/134),48.72%(38/78)and 25.00%(2/8)in gastritis,gastric ulcer and gastric cancer respectively.Among 67 isolates,23 strains 34.33%(23/67), were resistant to clarithromycin,and other stains were sensitive;No intermedial sensitive strain was found in these isolates.

结果:220例胃粘膜标本H.pylori培养阳性率为30.45%(67/220);胃炎培养阳性率为20.15%(27/134);消化性溃疡培养阳性率为48.72%(38/78);胃癌培养阳性率为25.00%(2/8);消化性溃疡组H.pylori培养阳性率明显高于胃炎组和胃癌组。67例H.pylori菌株克拉霉素耐药23株,占34.33%(23/67);敏感株44株,占65.67%(44/67);未发现克拉霉素中介株。

The ratio between nonsynonymous and synonymous substitutions for CP gene is 0.092 which indicated the negative selective pressure for amino acid changes. No distinct correlation was found among isolates in different years or different isolates in one year in a

灰飞虱的RAPO聚类分析结果与病毒CP基因的系统发育分析结果具有一些相似性,初步暗示了RSV与灰飞虱很可能是共同进化的。

Three infectious bronchitis virus isolates named as Q1, J2 and T3 were isolated from proventricular tissue taken from vaccinated chicken flocks in China The biological characteristics of the three isolates were observed.

从我国接种过传染性支气管炎疫苗的幼鸡群的腺胃组织中分离得到三个传染性支气管炎毒株Q1、J2和T3,并对它们的部分生物学特性进行研究。将病毒通过9-11日龄鸡胚,盲传5-6代。

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