查询词典 isolated element

Furthermore, there is a great phenotypic diversity among the new strains isolated from the same host. Different groups consisted of the new strains from the same host, some were Rhizobium, some were Bradyrhizobium, such as the new strains isolated from leguminous plants of AMkora Prain, Trifolium repens Lam, Aeachynomene Mica L, Indigofera bungeana Steud.

同时，攀西地区豆科植物根瘤菌的多样性还表现在某些来自同一种寄主植物根瘤菌的多样性方面，如分离自山槐、白车轴草、合萌、铁扫帚上的同一种寄主植物内的根瘤菌分别聚在不同的表观群；且来自山槐、合萌、铁扫帚上的根瘤菌既有快生型，又有慢生型。

Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.

基于以上两种重要疾病和研究组方向，本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作，累计筛选1万多个/次样品，发现10余个活性较强化合物；从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究，分离鉴定了58个化合物，发现6个新化合物和14个抗肿瘤活性化合物；通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究，发现它可能是通过降低G2期重要的转录因子E2F-1，致使细胞周期阻滞在G0/G1期，引起多发性骨髓瘤U266细胞凋亡；开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析，结果表明计算与实验一致。

Compound 4 was isolated from the genus Lonicera for the first time and all 5 compounds were first isolated from the species of this plant.

经文献检索，5个化合物均为首次从该种植物中分离得到，其中化合物4系首次从该属植物中分得。

Compounds Ⅰ,Ⅱ were first isolated from Genus Lonicera, while compounds Ⅲ~Ⅺ were isolated from the plant for the first time.

化合物Ⅰ、Ⅱ为首次从忍冬属植物中分离得到，化合物Ⅲ~Ⅺ为首次从该种植物中分离得到。

A pair of primers producing 614bp amplification fragment were designed based on the sequence of the C\|polyhedrin gene of Bombyx mori CPV,and the expected amplification products were obatined when genomic dsRNAs isolated from purified BmCPV,DsCPV and Lymantria dispar CPV were used as templates.Genomic dsRNAs isolated from Heliothis armigera CPV and DNAs from Lymantria dispar nuclear polyhedrosis virus and DNAs from midgut tissue of healthy Dendrolimus spectabilis larva did not yield any amplification products.The detection limit of purified DsCPV genomic dsRNAs was 1.0pg.

依据家蚕质型多角体病毒质型多角体蛋白的核苷酸序列设计一对引物，从纯化的DsCPV\，BmCPV和舞毒蛾质型多角体病毒的基因组dsRNA可成功地扩增出长614bp的目的片段，从提取的健康松毛虫幼虫肠组织的DNA、舞毒蛾核型多角体病毒基因组核酸、以及棉铃虫质型多角体病毒基因组核酸，未能扩增出目的片段。

Six compounds have been isolated from Lysimachia congestiflora, and ardicrenin (5) is isolated from this species for the first time.

从聚花过路黄中分离得到6个化合物，其中化合物(5)为首次从该植物中发现。

The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物，且所有试材的扩增结果一致，均无目的条带以外的产物扩增，说明Ty1-copia类逆转座子在苹果属植物中广泛存在，且存在历史可能较为久远；从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066)，结果表明，同一基因组来源的这些序列存在高度的异质性。

Analysis and fitting of growth curves in Liyang chicken;2. The results of antibacterial spectra and growth curves of eleven strains with better antagonistic effect showed that: 4 strains isolated from NAC plate had wider range of antibacterial spectra,3 strains isolated from manganic nutrient agar plate had advantage on growth.

用点种法从大黄鱼肠道优势菌群中筛选出104株病原性副溶血弧菌的拮抗菌，进一步研究拮抗效果最好的11株拮抗菌的抗菌谱和生长曲线，其中NAC平板上筛选的4株拮抗菌的抗菌范围较广，锰营养琼脂培养基上筛选的3株拮抗菌具有生长优势。

The number of autoterophic bacteria were significantly lower than the heterotrophic bacteria. Majority of 21 strains of heterotrophic bacteria that isolated from water body were Pseudomonas. Majority of 25 strains of heterotrophic bacteria isolated from sediment were bacillus, microccus and Pseudomonas.Three genus of autotrophic bacteria were sulphuret bacteria, nitrify bacteria and nitrosobacteria that were capable of oxidizing H2S, S, FeS, oxidizing NH3 to NO2 and NO2 to NO3 respectively.

试验稚鳖池水中共分离出21株异养细菌，其中优势菌为假单胞杆菌属细菌；底泥中共分离出25株异养细菌，其中优势菌为芽胞杆菌属细菌，微球菌属细菌假单胞菌属细菌，三种自养细菌分别为具有氧化硫化氢、元素硫或硫化亚铁的硫细菌；具有氧化氨为亚硝酸的亚硝化细菌和能将亚硝酸进一步转化成硝酸的硝化细菌。

In this paper, adopted augmenting culture in nitrite bacteria culture media and the method of silica gel plate isolation,31 strains of bacteria were isolated from vegetable garden soil of our school. The color reaction test was carried out with Griess reagent and culture fluid, which was regarded as the index to determine producing NO2- or not. 13 strains which color were deep were obtained and they were further rescreened by doing nitrite test. A strain N4(coded N4,the same to the following)with higher rate of nitrosification was picked up after rescreened; A strain B08( coded B08 ,the same to the following) with higher rate of denitrification was obtained after isolated and rejuvenated from our lab conserving mixed denitrifying bacteria culture.

本研究采用亚硝化细菌富集培养基选择培养和硅胶平板分离法，从本校农场菜园土中分离到31株细菌，以格利斯试剂对培养液的反应颜色深浅作为指标衡量其产NO_2~-的多少，经初筛从分离株中筛选出13株格利斯试剂反应颜色较深的菌株，再对这13株菌作亚硝化试验，最终选出一株亚硝化速率较高的菌株(编号为N_4，下同)；另外，通过对本实验室保存的反硝化细菌混合菌液进行分离复壮，筛选出一株反硝化速率高的菌株编号为B_

Yet, Edith says, there was always money in the bank.

但是Edith说，她的银行户口里面总是有钱。

We have created a series of files that resolve this problem--one for each day of the 40 days of purpose.

我们制作一系列的档案去解决这问题---一个为标竿40天中的每一天。