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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Explicitly, they are consist of three classes: If H is semisimple, then H k* for some finite group; If H is not semisimple and the characteristic of k is zero, then H is isomorphic the dual of the cross product between one so called Andruskiewitsch-Schneider algebra and a group algebra; If H is not semisimple and the characteristic of k is not zero, then H is isomorphic the dual of the cross product between one special algebra and a group algebra.

具体地讲，它们共分三类：①如果H是半单的，则H同构与一个群代数的对偶；②如果H是非半单的并且基础域的特征是0的话，则H同构一个所谓Andruskiewitsch-Schneider代数与一个群代数交差积的对偶；③如果H是非半单的并且基础域的特征不是0的话，则H同构于某个特定代数与一个群代数交差积的对偶。

I inquired that said these cultivations the seafood is mainly is iced up the influence, the supply is not big, but inshore fishing's silver pomfret fish is out of stock very much, the distant seas fishing for hairtail goods supply quantity is not big, by the small market saw frequently to eight belts, the goby and so on fresh small seafood, is only in the individual stall has the sale to freeze the small seafood, formerly could wholesale the solid stuff, did not have the trail now.

蛤蜊从每斤5元涨到8元，扇贝涨到了15元两斤，海蛎子、八带、虾虎等小海鲜的价格也出现了小涨，我打听说这些养殖的海鲜主要是受结冰影响，供应量不大，而近海捕捞的鲳鱼很缺货，远海捕捞的带鱼供货量也不大，以小市场经常看到八带、虾虎等新鲜小海鲜，只是个别摊位上有销售冰冻小海鲜的，以往能批发的干货，现在无踪迹了。

In this thesis the process of constructing the non-perturbative Hamiltonian theory is de-scribed and is applied to estimate the vacuum condensate. It contains the following contents:At the very beginning, by using the path integral method and eliminating the gluon freedom, aGCM action 〓 of current quarks including lower order current-current coupling was derivedfrom the QCD Lagrangian and the effective Hamiltonian operator that could hardly be doneby the normal methods was derived. After doing this, the broken vacuum is introduced whichincludes quark-antiquark condensate through the generalized Bogoliubov-Valatin transformation,the effective Hamiltonian of constituent quark was derived. The detailed formulas containingthe spatial current-current coupling term for the effective Hamiltonian and gap equations wasworked out by parameterizing the correlation kernel as a quadratic potential. And then, the gapequation was solved and the quark-antiquark condensate of vacuum was studied both in the casesof instantaneous interaction and retarded interaction. In the end, the effective Hamiltionian withtwo-body quark-quark interaction was derived with one-body approximation, and with the helpof the functional integral method the coupling non-linear dynamic equations for systems withnuclear matter was derived. Finally, these equations were solved by selfconsistent method andthe effect of nuclear matter on vacuum condensate was studied. The spatial current-current coupling term is too difficult to handle, hence the correlationkernel is assumed to be not important and usually omitted in the pure vacuum condensate, andthe instantaneous interaction generally is adopted. Retaining the spatial current-current termand partial retardation effect, the quark pairs condensate in pure vacuum was studied, and theeffect of quark mass was also studied. At present, little study is focused in the case with nuclearmatter and spatial current-current term also omitted. Under the approximation with partialspatial current-current term, the effect of nuclear matter on vacuum condensate was studied.

本论文描述了量子色动力学整体色对称模型哈密顿量方法的构建过程，得到了反映正反夸克对凝聚真空结构的关于组分夸克的有效哈密顿量算符，它隐含了胶子作用，并且准确至流-流耦合项；接着，通过参数化哈密顿量中的夸克作用关联核，导出平方禁闭势参数化选择的哈密顿量的具体公式和能隙方程；随后，应用公式，编程求解，考察了瞬时作用下和部分延迟作用下真空的正反夸克对凝聚，在计算中保留了空间流-流耦合作用；之后，导出瞬时势和延迟势下包含二体作用项的哈密顿量公式，并采用单体化近似，通过泛函变分方法得到核物质存在时耦合的非线性动力学方程；在保留部分空间双流耦合作用的近似下，求解核物质的动力学方程，考察核物质密度对真空凝聚的影响，以往考察真空凝聚，对关联核的选用，由于空间流-流耦合项不易处理，也认为作用不大，常忽略该项，并且常采用瞬时作用；本文保留空间双流项和部分延迟作用，考察了真空情形的夸克对凝聚，还考察了夸克质量对纯真空凝聚的影响，以往对核物质存在情形的真空凝聚考察很少，也都忽略空间流-流项，本文在考虑部分空间流-流项近似下，考察了核物质存在对真空凝聚的影响。

But never was a fight managed so hardily, and in such a surprising manner, as that which followed between Friday and the bear, which gave us all (though at first we were surprised and afraid for him) the greatest diversion imaginable: as the bear is a heavy, clumsy creature, and does not gallop as the wolf does, who is swift, and light; so he has two particular qualities, which generally are the rule of his actions; first, as to men, who are not his proper prey; I say, not his proper prey; because though I cannot say what excessive hunger might do, which was now their case, the ground being all covered with snow; but as to men, he does not usually attempt them, unless they first attack him: on the contrary, if you meet him in the woods, if you don't meddle with him, he won't meddle with you; but then you must take care to be very civil to him, and give him the road; for he is a very nice gentleman, he won't go a step out of his way for a prince; nay, if you are really afraid, your best way is to look another way, and keep going on; for sometimes if you stop, and stand still, and look steadily at him, he takes it for an affront; but if you throw or toss any thing at him, and it hits him, though it were but a bit of a stick, as big as your finger, he takes it for an affront, and sets all his other business aside to pursue his revenge; for he will have satisfaction in point of honour; that is his first quality: the next is, that if he be once affronted, he will never leave you, night or day, till he has his revenge; but follows at a good round rate, till he overtakes you.

再没有什么战斗比"星期五"和大熊之间后来那场战斗更勇敢和更惊险的了。这场大战起初确实使我们感到很惊险，但最后却给了我们大家无比的娱乐。熊这种动物身体笨重，行动迟缓；不像狼那样一路小跑，又快又轻。因此熊具有两个特性，作为它的一般行动规则：第一、先说人，人不是熊的合适的猎物，我是说的"合适的猎物"；当然，像现在这种情况，地上全被大雪覆盖，熊极端饥饿时是否也会吃人，我就不敢说了。不过，人一般不要去攻击熊，除非熊首先攻击人；相反，要是你在树林里遇到熊，你不去惹它，它也不会惹你。不过你得特别小心，要对它特别客气，给它让路，因为它是一位非常可爱的绅士，就是王子，熊也不会给他让开一步的；如果你真的害怕，最好不要看它，继续走你的路。如果你停下来，站着不动，盯着看它，它就认为是对它的挑衅。如果你向它扔什么东西，打中了它，哪怕是一根只有手指头粗的棍子，它也认为是对它一种挑衅，就会不顾一切，实行报复；因为只有把荣誉挣回来他才会感到满足。这是熊的第一个特性。第二个特性是：熊一旦受到挑衅，就会日夜跟着你，直到报完了仇；哪怕绕上许多路，也要跟着你，直到把你抓住。

But never was a Fight manag'd so hardily, and in such a surprizing Manner, as that which follow'd between Friday and the Bear, which gave us all (though at first we were surpriz'd and afraid for him) the greatest Diversion imaginable: As the Bear is a heavy, clumsey Creature, and does not gallop as the Wolf does, who is swift, and light; so he has two particular Qualities, which generally are the Rule of his Actions; First, As to Men, who are not his proper Prey; I say, not his proper Prey; because tho' I cannot say what excessive Hunger might do, which was now their Case, the Ground being all cover'd with Snow; but as to Men, he does not usually attempt them, unless they first attack him: On the contrary, if you meet him in the Woods, if you don't meddle with him, he won't meddle with you; but then you must take Care to be very Civil to him, and give him the Road; for he is a very nice Gentleman, he won't go a Step out of his Way for a Prince; nay, if you are really afraid, your best way is to look another Way, and keep going on; for sometimes if you stop, and stand still, and look steadily at him, he takes it for an Affront; but if you throw or toss any Thing at him, and it hits him, though it were but a bit of a Stick, as big as your Finger, he takes it for an Affront, and sets all his other Business aside to pursue his Revenge; for he will have Satisfaction in Point of Honour; that is his first Quality: The next is, That if he be once affronted, he will never leave you, Night or Day, till he has his Revenge; but follows at a good round rate, till he overtakes you.

紧接着，星期五和那只大熊之间展开了一场最大胆、最惊人的大战。这场大战起初确实使我们胆战心惊，最后却使大家开怀大笑。熊的身体笨重，行动蹒跚，跑起来当然没有狼那样轻快。因此，他的行动有两个特点。第一，对人来说，他一般不把人当作猎食的对象；当然，像现在这样大雪遍地，极端饥饿的时候，这笨拙的大家伙是否也会吃人，那就很难说了。一般来说，要是在树林里遇到熊，你不去惹它，它也不会来惹你。不过，你得特别小心，要对它客气，给它让路，因为它是一位特别难以取悦的绅士，即使是一位王子走来，它也不肯让路。如果你真的害怕，最好不要看它，继续走你的路。如果你停下来，站着正视它，熊就认为是对它的侮辱。如果你向它丢点什么东西，打中了它，哪怕是一根小小的树枝，只有你手指头那么粗，熊也认为是一种侮辱。这时，它会把一切丢开不管，一心只想报仇，不达目的决不罢休。这有关它的荣誉问题，它一定要把面子挣回来才算满足。这是熊的第一个特点。第二个特点是，熊一旦受到侮辱，就会不分昼夜地跟着你，一直到报了仇才罢休，哪怕绕上许多路，也要赶上你，抓住你。

The theory and application is explained in this paper. Software XMGREDIT5 matching ELCIRC is used to generate unstructured grid, and is considered fit for ocean but rivers. Tidal current is simulated and validated well, and sensitivity of current structure to bathymetry is visible. Affection of wind is qualitatively analyzed here. At the same time the model is improved on as required, like the tidal open boundary which could choose not only long period harmonic constant but also short period harmonic constant and tidal level, and multi-point results and flow field data could be exported effectively with binary results.

本文介绍了ELCIRC的理论及使用；基于其在Linux平台下的软件XMGREDIT5生成全三角形网格并评价其特性，认为XMGREDIT5较适合大范围海域而不适用于河道；应用ELCIRC模型计算了长江口的潮流场并进行验证，结果与实测资料吻合良好，且能较好地模拟出地形变化对水流结构的影响；文中对于风场的影响作了定性分析，同时根据实际需要对模型作了改进，如将仅支持长期资料调和常数的开边界条件改为可选择的短期调和常数边界和潮位边界，使二进制格式结果文件数据输出可以处理多点数据及流场等等。

The city is different from an enterprise, the enterprise is an economy system merely, what go after consequently is profit is the biggest change, the partner of the enterprise is evaluated to its basically also is from profit have sth in mind.

城市不同于企业，企业仅仅是一个经济体，因而追求的是利润的最大化，企业的股东对其评价也主要是从利润着眼。

The road of Kaoyan is fruitful, for it is a headful of knowledge you have gained; the road of Kaoyan is pleasant, for it is a small step forward you have made; the road of Kaoyan is worthwhile, for it is the youth you have cherished.

考研之路是充实的，因为每天你都收获了满脑的知识；考研之路是幸福的，因为每天你都朝目标迈进了一小步；考研之路是值得的，因为你的青春没有留下遗憾；在考研路上现在你是成功者，因为每天你都在超越昨天的自己。

I am a high school girl, the logistics work in building materials for two years, and later to provide training to fashion design, it is to do this work, because nothing like us to do work experience is very difficult to find work So it more difficult to find if not a very good, so people are looking for挺多relations as it would have been better points, can also have the feeling of Recently, however, I encountered an abnormal designers, because at that time I also have to go to his That interview, he was a design director吧He told me I was with him did not go to school because we feel that a girl I was a bit inconvenient for the kind of timid that do not understand that person is not at ease to get there, and later their own into the a plant is not very good, I know that we have to slowly, but it results only knew he was in front of his girlfriend said I was his work in order to seduce him (Even more is that his girlfriend is I am an alumnus), he did slander me, I really gas hematemesis, his girlfriend that he cheated, he did not dare to him are so angry because he designed to help local (he did not admit that I am an alumnus his girlfriend is also everywhere to deceive the girls), but I do not like what I scolded him a big deal then I am leaving this line.

我是一个高中毕业的女孩，在建材物流工作了两年了，后来去培训服装设计，现在也是做这块工作呢，，因为像我们这样没什么工作经验的人是很难找到工作的呢所以比较难如果找到也不是太好的，所以挺多人都是找关系的呢那样会比较好点，本来感觉还可以的可是最近我遇到一个变态的设计师，因为当时我也有去他那公司面试，他是个设计总监吧他叫我跟他学我那时没去因为觉得一个女孩子不方便我比较胆小的那种认为不了解那个人就不放心去呢，后来自己进了一家厂也不是很好的呢，我知道要慢慢来，可是呢结果才知道他在他女朋友面前说我那时为了去他那工作勾引他呢(更巧的是他的那个女朋友是我的校友)，他那样诽谤我，真的把我气吐血呢，他女朋友知道他骗他又不敢对他生气因为都是做设计的要他帮忙的地方(他都不承认我的校友是他的女朋友还到处去骗女孩子)，可是我就不一样了我骂了他大不了我不干这行呢。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。