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The PCR protocol involved 35 cycles of denaturation at 94 ℃ for 3 minutes and then elongation at 72 ℃ for 10 minutes.

反应条件为94 ℃变性3 min后进行35个循环，最后72 ℃延伸10 min。

There was no question of Margaret having been involved in the breakdown of Denis's marriage.

玛格丽特与丹尼斯的第一次婚姻破裂毫无关系。

Denise:It's a lot harder and there's a lot more work involved.

丹尼斯：那做起来要更难，有更多的工作需要去做。

This time the denouement would be one of the biggest bubbles in history, probably in scale and certainly in number of people involved.

这次将以史上最大的泡沫而结束，可能波及到很大的规模和数量的人。

Objective: To study the cerebellar dentate nucleus' shape and size, in order to provide anatomical basis of dentate nucleus involved in the diagnosis and treatment of diseases.

目的：明确小脑齿状核的形态和大小，为涉及齿状核的疾病的诊断和治疗提供解剖学基础。

Providing dentate nucleus shape,and showing the reference of the size of dentate nucleus value in order to provide anatomical basis of dentate nucleus involved in the diagnosis and treatment of diseases.

提供齿状核的形态，显示齿状核的大小的参考值，为临床涉及齿状核的疾病的诊断和治疗提供依据。

Lack of policy coordination mechanisms result in departmentalization of national obligations, and the major executing department of the national policy has not been adequately involved in formulating these GSP policies.

文章列举若干惠民政策，总结其对计划生育政策带来的不利影响，并根据和谐社会"公平正义"的目标从政策过程理论角度分析了这种现象的制度成因：未建立有效的政策协调机制，以致"国家义务部门化"，基本国策的主要执行部门在这些政策的制定和实施中参与力度不够。

The governing equations of laminate-faced sandwich cylindrical shallow shells based on the first-order shear theory with large deflection are high-order partial differential equations. Four dependent functions are involved.

大挠度剪切理论下复合材料夹层圆柱扁壳的稳定性控制方程是一组非线性高阶常系数偏微分方程，其中包含四个独立的函数，它们分别为横向挠度w、参考曲面的法线转角Φx、Φy和应力函数F。

The results suggest that protein kinase A may be involved in the ethanol inhibition of NMDA-induced depolarization.

由此可推测，蛋白质激酶A可能参与乙醇抑制NMDA所引发之去极化作用。

Banking elites involved in a global depopulation policy, then it becomesdepopulation program by the North American branch of the Illuminati.

如果富尔福德是准确的，在有关他的主要要求的决心和中国秘密社会目标的能力在全球销毁政策涉及银行精英的话，那就表明亚洲秘密会社

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。