查询词典 investigate and survey

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

This paper introduces the outline of inside tunnel survey,and expounds the survey control in tunneling works from aspects of the construction traverse survey,midline layout survey,midline detection and tolerance,elevation control survey,vertical section survey and cross section survey,and breakthrough survey,etc.

介绍了洞内测量纲要，从施工导线测量、中线施工放样、中线检测与限差、高程控制测量、纵断面测量和横断面测量、贯通测量等方面阐述了隧洞工程测量控制。

Province, Shijiazhuang, Baoding have been involved in two rounds of 61 cities nationwide survey and synthesis of MSM epidemic prevention and control work, 4-6 months of this year the third round of the survey will be carried out; the rest of the city of nine work-site investigations and Shijiazhuang, Baoding, the third round of the survey the same time, also in accordance with the national program of 61 pilot cities, the survey used a snowball method, sample size of 300 people, mainly MSM population survey of AIDS, syphilis, hepatitis C infection; Shijiazhuang, Baoding, the completion of the third round of the national survey At the same time, will also focus on the MSM population in rural areas of the outbreak investigation; in July 2009, municipalities to carry out data entry, upload, analysis and summary.

我省石家庄、保定已参与两轮全国61个城市MSM疫情调查与综合防治工作，在今年4-6月份将开展第三轮调查；其余9市的现场调查工作与石家庄、保定的第三轮调查同步进行，同样按照国家61个试点城市方案、采用滚雪球法调查，样本量均为300人份，主要调查MSM人群艾滋病、梅毒、丙肝的感染情况；石家庄、保定在完成全国第三轮调查的同时，还将侧重于农村地区的MSM人群的疫情调查；2009年7月，各市进行数据录入、上传、分析和汇总。

The background survey and tracking survey of artificial reef area in Chenghai Laiwu were carried out before and after reefing in 2003 and 2007, respectively. The results indicated that after the deployment of artificial reefs, the biomass density of fishery resources increased by 25.63 times, which were much higher than before. The resources species was much more abundant; total number increased from 23 to 41, which increased by 0.78 times. The crab resources sharply increased by 1.75 times after artificial reef were deployed. Harpodon nehereus and Portunus sanguinolentus , which did not appeared in background survey, now became the dominant species in tracking survey. There were high diversity indexes in artificial reefs area and control area in tracking survey. In conclusion, the results indicated that the fish-aggregating effect of artificial reef site has improved a lot, and the community structure and the habitat environment have been significantly improved.

摘 要：2003和2007年分别对澄海莱芜人工鱼礁区进行了投礁前的本底调查和投礁后的跟踪调查，结果表明，投礁后礁区海域游泳生物的资源密度明显比投礁前高，增加了25.63倍；礁区海域各类资源种类均比投礁前丰富，总种数由投礁前的23种增加至41种，比投礁前增加了0.78倍，其中，蟹类种数增加最多，增加了1.75倍；在本底调查中没有出现的经济种类龙头鱼 Harpodon nehereus 和红星梭子蟹 Portunus sanguinolentus 在跟踪调查中已成为主要优势种；Shannon-Wienver多样性指数在礁区和对比区均比投礁前有所增加。

First, the survey process, including the sampling procedure and design of the survey questionnaire, increases the risk that the survey will collect nonrepresentative data from the survey population, thereby limiting the overall representativeness of the resulting survey data.

首先，调查过程，包括抽样程序和设计的调查问卷，增加了风险，这项调查将收集数据nonrepresentative从调查的人口，从而限制了整体的代表性，由此产生的调查数据。

Secondly, summarized the management system of part-time faculty in Zhongqiao, pointed out the experiences and disadvantages through comparing by using many methods like questionnaire、 interview and statistic to make a further survey on aspects of part-time faculty management status quo: questionnaire survey on employing management, compensation, motivation and performance evaluation and the expectation and evaluation of the student to the teacher; interview survey on the bepuzzlement and measure of administrators; statistic survey on performance evaluation, liquidity and compensation of the part-time faculty.

其次概述了上海中侨学院兼职教师的管理制度，并通过调查问卷、访谈和数据统计等方法对上海中侨学院兼职教师的实际管理现状作了深入调查研究。在兼职教师的聘任情况，福利状况，激励现状，管理情况和学生对兼职教师的评价和期望等方面进行了调查研究；在学校管理者对兼职教师管理上的困惑和解决措施等方面进行了访谈研究；在兼职教师的绩效评估、流动性、薪资福利等实际管理情况进行了统计分析。

Therefore, the 3S technologies are fully applied in the area. On one hand, remote sensing mapping and digital elevation modeling are used to realize highprecision seismic data acquisition and quality control of field operation. GPS control points are emplaced through "firstorder control, survey grid placement stepbystep and overall adjustment", and surface fitting technique is used to build a digital model with regional height anomaly corrected. The GPS RTK and fullstationequipment traverse survey are used to realize the lofting survey of shooting and receiving points. The height fitting of shooting and receiving points of the GPS RTK survey is realized through correcting the digital model with regional height anomaly.

为此，全面地采用了3S技术，一方面从总体上运用卫星遥感影像成图技术和数字高程模型系统，实现室内对地震勘探的精确设计和对野外施工质量过程监控；用GPS卫星定位技术采取&首级控制、分期布网、首分网整体平差&方法布测物探GPS控制点，采用曲面拟合技术建立区域高程异常改正的数字模型；应用GPS RTK和全站仪导线测量等方法实现激发、接收点放样测量，利用区域高程异常改正数字模型对GPS RTK测量的激发、接收点进行高程拟合。

The paper introduces the latest development and application in the production of engineering survey technology. GPS allocation technology and the combination of GPS and total station have been widely used in plane surveying of control survey. Electron distance trigonometric leveling can replace third and fourth-order spirit leveling in vertical survey. Data collection, processing and drawing by total station has achieved automation and integration in topographic survey, etc.

随着工程测量技术的发展，控制测量中平面测量已广泛地采用了GPS定位技术、GPS与全站仪相结合的技术，高程测量中电子测距三角高程能代替三、四等几何水准测量；地形图测绘中，实现了全站仪从野外数据采集、处理到绘图过程的自动化和一体化；摄影测量和高分辨率遥感技术在大比例尺地形测图中的应用等。

Taking the horizontal control survey of Qinling Tunnel as an example and on the premise of the survey design,the paper presents in detail the technical points and arrangement methods of the horizontal control survey inside long cut-through tunnels and the concrete requirements and items to which attention should be paid during the survey.

0前言隧道贯通误差在铁路测量规范中有着明确的限差规定，它是衡量隧道正确施工和控制测量精度的重要指标。

The data were collected from the public communities in random sampling and college students in subjective sampling. The survey were conducted in three stages, including pre-survey, pilot survey and main survey; response rate was 71%; and 956 valid copies of questionnaire were collected.

以社会公众和在校大学生作为研究对象，分别采用方便抽样和主观抽样，进行了三阶段的问卷调查，包括预试、初试和主试，问卷回收率71%，共收集有效问卷956份。

Some of them were ligated to the plasmid vector pCR 2.1 andsequenced,respectively.

将所获DNA片段克隆到pCR2.1载体进行测序，并与基因组数据库进行同源性比较。

Thinking since Google owns Blogger, I would be able to escalate this issue to the support team.

自谷歌的思考拥有Blogger的，我将能够缓解这一问题的支持团队。