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It has inserted a clause, for instance, that in effect ensures Japan's cosseted farmers would be exempt from any free-trade deal with America.

例如加入了一项条款：着实确保了日本农民可以享有不必遵守美国自由贸易条款的特权。

Through adopt the measure such as controling status of hot metal (for example content of carbon, content of oxygen, amount of converter slag, weight of hot metal etc.), depressing degree of vacuum promptly by driving vacuum pump ahead or increasing flow of nitrogen, controling correctly inserted deepness of duck, controling the time of decarburizing, process parameters of decarbonization for managing IF steel using RH—MFB equipment are optimized.

通过严格控制来钢条件，如碳、氧含量，转炉下渣量，钢水量；迅速降低真空室内真空度，采用预真空的压降模式，提高提升气体流量；保证浸渍管插入深度及脱碳时间等措施，优化了攀钢RH—MFB处理超低碳IF钢的脱碳工艺参数，取得了较明显的工艺效果。

Xiadu a glass of wine, white smoke will be unconscious, woke up to find that their suffering has been deflowered, is different from Viagra in the past and the mountains, this time, she felt pain, like a knife inserted into the straight, which things out Out, she has been confused and disoriented.

一杯酒下肚，白烟便不省人事，醒来时痛苦地发现自己已然失身，只是不同于从前的伟哥和山海，这一次，她感觉疼痛，似是刀插直入，当哪条东西拔出来之时，她已晕头转向。

Stamens inserted at apex of corolla tube; filaments short, equal; anthers elliptic dehiscing by oblique apical pores.

雄蕊花冠筒的着生的在先端；花丝短，等长；花药椭圆形以偏斜的顶孔开裂。

This dummy is a control for the local-bias effect of Coval and Moskowitz (1999); it is effectively differenced out in our trades specifications, but needs to be inserted when working with holdings.

这个道具是控制当地偏见的影响， coval和莫斯克维兹（ 1999年）；它是有效的差分列在我们的行业规格，但需要插入工作时，与控股。

Those showed that the E chromosome of thinopyrum intermedium inserted the genome of common wheat, and it disarranged the original matching of chromosome.

这些现象表明中间偃麦草的E组染色体进入了普通小麦的基因组，影响ABD组染色体配对。

Methods:One hundred and six disarticulated human verebrae(C3～C7)were evaluated with computed tomography to determine vertebral bone mineral density.Pedicle screws and lateral mass screws were inserted to the left and right at random.Screws were loaded either axially strictly longitudinal to failure or in a coronal plane strictly perpendicular to the logitudinal axis,the strengths and displacements were recorded.

方法解离C3～C7骨块120个随机分为两组，测量每个椎体骨的松质骨密度，同一节段椎体随机配对置入椎弓根和侧块螺钉，在位移控制下分别测量位移控制下的平均最大拔出阻力和压力控制下的切向弹性与永久位移。

The P32 gene of capripoxvirus were amplified by PCR.The product of PCR was cloned to the plasmid pGEM-T-Easy.Then the P32 gene was disparted to three parts.Three parts of P32 gene were inserted into the expressed plasmids pGEX-4T-1, which were identified by PCR and restriction enzyme analysis.

本研究利用PCR方法对羊痘病毒P32蛋白基因扩增，将回收PCR产物克隆到pGEM-T-Easy载体中，然后将P32蛋白基因分成3段，分别插入原核表达载体pGEX-4T-1中，经PCR和酶切鉴定，证明成功构建了重组表达载体pGEX-4T-1-N2/3、pGEX-4T-1-N1/3、pGEX-4T-1-M。

METHODS The HCV core gene coding region was inserted into the eukaryotic expressive plasmid pcDNA3, then the recombinant plasmid pcDNAHCV-C was constructed and expressed transiently with LipofectAMINE in the SP2/0 cells. After purification, these plasmid directly or encapsuled wit h Lipofect-AMINE were injected into BALB/c mice. HCV core antibody from immunized mice could be detected by ELISA. RESULTS The enzyme -cutting identification showed that HCV core gene fragment had been cloned into pcDNA3 eukaryote vectors.

人工构建包含HCV C基因片段的真核表达载体pcDNAHCV-C，在证实其可以在真核细胞中表达之后，将其用脂质转染剂包裹，形成LipofectAMINE-pcDNAHCV-C脂质混合物，将该混合物或单纯pcDNAHCV-C直接注射BALB/c小鼠股四头肌，以空载体pcDNA3做对照，ELISA法检测血清中抗体产生水平。

Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

For the head-teacher of the class said that I had seriously broken the school rules,which led me to a three-day suspend class.

为什么呢？因为我的班主任说我严重的违反了校规，于是让我停课三天。

Many of them believe that the conversion of thousands of working-class folk in England spared that nation from the mass carnage and the tyranny that came with the revolution in France.

他们之中有许多人相信，在英国数以千计的劳工阶级之悔改信主使英国免於遭受如法国大革命所造成的大屠杀和专制暴政。