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The glucoamylase I gene was inserted into the pAC1 plasmid,and a expression plasmid was constructed,then it was used to transform the yeast strain, S.cerevisiae AS2.1364,without auxtrophic marker,The amylolytic tests showed that when this transformant was incubated on plate of YPDS medium containing 1% glucose and 1% starch at 30℃ for 48 hrs zones of starch degradation could be visualized by stained with iodine vapour.

这说明酵母转化子不仅表达了该酶，且有效地分泌到胞外，从而水解培养基中的淀粉，进一步测定酵母转化子48hrs发酵液的葡萄糖淀粉酶活力为8.4u/ml，培养基中的淀粉已完全水解，其生物产量较高。

Patients and Methods: Seventy patients having 71 OTA 32 fractures were randomly allocated into 2 groups to be treated with either antegrade or retrograde intramedullary nails inserted with reaming.

病人和方法：70个病人（71 OTA 32骨折）被随机分配至两组，分别采用顺行或逆行髓内针治疗。

Staminodes apically dumbbell-shaped; fertile stamens 2 or 3, inserted in posterior or anterior position.

顶部的退化雄蕊是哑铃形；可育雄蕊2或3，着生于后唇瓣或前面的位置。

Drupes with a narrow, apically inserted wing; stipular spines 1 or 2 per node

核果的具一个狭窄，顶部着生的翅膀；托叶刺1或2 每节点 2

Stamens shorter than sepals and petals, inserted on lateral surface of ovary; filament linear to ovate or obovate; anther connective appendaged or unappendaged.

雄蕊短于萼片和花瓣，子房的着生于侧面；花丝线形到卵形或倒卵形；花药药隔附属物或无附属物。

Stamens erect, inserted at base of perianth segments; filaments free or basally ± connate; anthers basifixed, apex often adaxially appendaged; connective extended into a perianthlike, linear-lanceolate, long appendage.

雄蕊直立，着生的在花被片基部；花丝离生或基部多少合生；花药基着，先端通常附属物；药隔延长至花被状，线状披针形，长附属物。

This is not a fatal disease, however, it may lead to serious complications such as chronic cystitis, urolithiasis, or rectal abscess formation. These foreign bodies were inserted for autoerotic or unknown reasons by patients.

虽然膀胱异物并不会造成患者的死亡，但是却会引起严重并发症如膀胱炎、结石等，甚至有些异物还会由膀胱较弱的地方穿出膀胱至直肠或腹腔内，引起直肠脓肿或急性腹症。

RESULTS: The DNA sequence of rLL37 was inserted into vector pET30a and expressed in E.coli BL21 star(DE3). Gel scanning analysis showed that fusion protein accounted for 35% of total bacterioprotein.

结果： 改良的LL37多肽于载体pET30a在杆菌BL21 star(DE3)中高效融合表达，用凝胶扫描显示融合蛋白的表达量约占全菌蛋白的35％。

Stamens 2 × as many as petals, inserted basally on corolla.

雄蕊2 *同数花瓣，着生在上花冠。

Stamens 6, inserted at base of tepals; anthers basifixed, rarely dorsifixed.

雄蕊6，着生的在花被片基部；花药基着，很少背着。

"The operators will suffer a temporary decrease in the number of consumers and short message services as people may not like having to provide their ID cards for a phone number," Xu Junqi, vice-dean with the Policy-Making Institute of the Telecommunications Research Centre under MII, said yesterday.

信息产业部下属的通信决策研究院副主任徐俊其昨天说：&实行手机实名制后，人们可能不太情愿用身份证注册手机号，所以通信运营商会暂时面临用户减少和短信用户减少的问题。&

I don't believe in make-believe!

我不相信这些虚构的故事。