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The subacutely aging rats were made by injecting Dgal into the rats' abdominal cavity continuosly. The learning and memory ability of rats in each group were detected with Morris water maze. The monoamine transmitter contents in hypothalamus and hippocampus were detected with HPLCECD.

D半乳糖连续腹腔注射制作亚急性衰老的大鼠模型，采用Morris水迷宫检测各组大鼠的空间学习记忆能力及采用高效液相色谱电化学法检测各组大鼠下丘脑、海马NE，DA，5HT的含量。

Methods The model of subacutely aging rats were made by injecting Dgal into the rats, abdominal cavity continually. After lavaging HSWY, the apoptosis rate and the levels of Fas and FasL mRNA were measured by TUNEL and RTPCR; the expressions of Fas/Fasl protein were detected by SP immunocytochemistry.

采用D半乳糖连续腹腔注射致亚急性衰老大鼠模型，同时以何首乌饮灌胃作为延缓衰老组；造模后应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测各组大鼠卵巢细胞凋亡情况；测定各组Fas和FasL的信使核糖核酸水平；SP免疫组化法检测卵巢细胞Fas和FasL蛋白的表达情况。

Photomicrographs of retrogradely labeled neurons in the trigeminal ganglion after Fast blueand Nuclear yellow injecting into the caudal subnucleus of spinal trigeminal nucleusand the nucleus of the solitary tract,respectively.

图A、B分别注射Fast blue和Nuclear yellow入三叉神经乙肝脊束核尾侧亚核和孤束核后三叉神经乙肝节内的逆行标记孩子细胞。

Methods: A rat chronic gastric ulcer model was made by infecting Hp(NCTC11637 strain)after injecting acetic acid into the subserosa of the stomach. In the experiments, the techniques of immunohistochemical stain and in situ hybridization etc. were used to observe the expression of bFGFmRNA and PCNA in th ulcer.

采用幽门螺杆菌多次重复感染乙酸性胃溃疡大鼠，造成Hp相关的慢性胃溃疡模型，运用免疫组化和原位杂交等技术观察该方对Hp感染的慢性胃溃疡大鼠胃黏膜碱性成纤维生长因子mRNA和胃黏膜细胞增殖核抗原的影响。

A rat model induced by injecting acetic acid into the subserosa of stomach was used.Uicer index was measured,the level of nitri oxide in serum and plasma endothelin-1(ET-1) was examined.

采用大鼠乙酸性胃溃疡模型，分别以消溃灵灌胃和左旋精氨酸、亚硝基左旋精氨酸腹腔内注射治疗7 d和14 d后，测定溃疡指数、溃疡抑制率，检测血清一氧化氮及血浆内皮素(ET-1)含量。

Methods SAP was induced in SD rats by injecting 5% sodium taurocholate(0.15 ml/100 g) under pancreatic capsule.

SD大鼠24只随机分成2组，对照组与SAP组各12只。

ASP model was established by injecting with 35 g/L taurocholic acid into the biliopancreatic duct.

选取雄性SD大鼠48只，分为ASP实验组（24只）、对照组（24只）。

ASP model was established by injecting with 35 g/L taurocholic acid into the biliopancreatic duct. Both groups were sacrificed at 3, 6 and 12 h with 8 rats at each time point. At scheduled time, bronchoalveolar lavage fluid was drawn and PMNs were then obtained by gradient density gradient centrifugation method. ratio of apoptosis and necrosis of PMN was assayed by flow cytometry and LDH as well as index of lung permeability were measured.

实验组大鼠穿刺胰胆管并注入35 g/L牛磺胆酸钠制作ASP大鼠模型，分别在制模后3， 6， 12 h剖杀，于预定时相取支气管灌洗液，密度梯度离心分离PMN，用流式细胞仪测定PMN凋亡、坏死比例，同时测定乳酸脱氢酶含量、肺通透指数。

ASP model was established by injecting with 35 g/L taurocholic acid into the biliopancreatic duct. Both groups were sacrificed at 3, 6 and 12 h with 8 rats at each time point. At scheduled time, bronchoalveolar lavage fluid was drawn and PMNs were then obtained by gradient density gradient centrifugation method. The ratio of apoptosis and necrosis of PMN was assayed by flow cytometry and LDH as well as index of lung permeability were measured.

实验组大鼠穿刺胰胆管并注入35 g/L牛磺胆酸钠制作ASP大鼠模型，分别在制模后3， 6， 12 h剖杀，于预定时相取支气管灌洗液，密度梯度离心分离PMN，用流式细胞仪测定PMN凋亡、坏死比例，同时测定乳酸脱氢酶含量、肺通透指数。

ASP model was established by injecting with 35 g/L taurocholic acid into the biliopancreatic duct. Both groups were sacrificed at 3, 6 and 12 h with 8 rats at each time point. At scheduled time, bronchoalveolar lavage fluid was drawn and PMNs were then obtained by gradient density gradient centrifugation method. The ratio of apoptosis and necrosis of PMN was assayed by flow cytometry and LDH as well as index of lung permeability were measured.

实验组大鼠穿刺胰胆管并注入35 g/L牛磺胆酸钠制作ASP大鼠模子，分别在制模后3， 6， 12 h剖杀，于预定时相取支气管灌洗液，密度梯度离心疏散PMN，用流式细胞仪测定PMN凋亡、坏死比例，同时测定乳酸脱氢酶含量、肺通透指数。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。