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The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。

There were significant differences of the incidences of 4 water-borne infectious diseases(cholera,hepatitis A,bacteroidal diarrhea and typhoid or paratyphoid)between former 5 years and later 5 years.The incidences of 4 water-borne infectious diseases except bacteroidal diarrhea showed downtrends year after year in 10 past years.By relativity analyzing,there were negative correlations between overall incidences of 4 water-borne infectious diseases and the qualification rate of drinking water coliform group,between the incidence of hepatitis A and drinking water coliform, between the incidence of typhoid or paratyphoid and drinking water total plate countand between the incidence of typhoid or paratyphoid and drinking water coliform.

改水前后各五年霍乱、甲肝、菌痢、伤寒副伤寒等四类主要介水传染病总发病率的差异有显著性,四类主要介水传染病的总发病率与饮用水大肠菌群合格率之间、甲肝发病率与饮用水大肠菌群合格率之间、伤寒副伤寒发病率分别与同期饮用水细菌总数和大肠菌群指标合格率之(来源:A1eBC3737论文网www.abclunwen.com)间呈负相关。

Article 3 Infectious diseases specified in this Law shall include quarantinable infectious diseases and infectious diseases to be monitored.

第三条 本法规定的传染病是指检疫传染病和监测传染病。

Infectious diseases specified in this Law shall include quarantinable infectious diseases and infectious diseases to be monitored.

第三条 本法规定的传染病是指检疫传染病和监测传染病。

Article 3 infectious diseases specified in this Law shall include quarantinable infectious diseases and infectious diseases to be monitered.

第三条 本法规定的传染病是指检疫传染病和监测传染病。

Article 3 Infectious diseases specified in this Law shall include quarantinable infectious diseases and infectious diseases to be monitored.

第三条 本法规定的传染病是指检疫传染病和监测传染病。检疫传染病,是指鼠疫、霍乱、黄热病以及国务院确定和公布的其他传染病。

Article 3 Infectious diseases specified in this Law shall include quarantinable infectious diseases and infectious diseases to be monitored.

检疫传染病,是指鼠疫、霍乱、黄热病以及国务院确定和公布的其他传染病。

New infectious disease ; emerging infectious disease ; reemerging infectious disease ; human health

新兴传染病;新现传染病;再现传染病;人体健康

New infectious diseases, including both emerging infectious diseases and reemerging infectious diseases, have raised growing worldwide concerns.

新兴传染病」受全球关注已有相当时日,所谓「新兴传染病」包括新现传染病及再现传染病两类。

Group C report the incidence of infectious diseases Habitat top three diseases were: other infectious diarrhoeal diseases, hand, foot and mouth disease and mumps, or C incidence of infectious diseases reported a total of 98.5 percent.

丙类传染病报告发病数居前三位的病种为:其它感染性腹泻病、手足口病和流行性腮腺炎,占丙类传染病报告发病总数的98.5%。

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