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Sequence analysis and phylogenetical study showed that this cDNA contained 778 nucleotides, with a 627 bp open reading frame flanked with a 44 bp 5' UTR and a 107 bp 3' UTR. The encoded 208 amino acids housefly Attacin shared a high similarity of 50%~70% with that of the other dipterous insects. In addition, the phylogenetical analysis also indicated that the Attacin from housefly was in the same branch with those of other species, suggesting that they come from the same ancestor.

家蝇Attacin的cDNA全长778 bp,其中包括627 bp的开放式读码框,44 bp的5ˊ末端非编码区(5ˊUTR)和107 bp的3ˊ末端非编码区(3ˊUTR),相应的蛋白产物含208个氨基酸;与其他双翅目昆虫的Attacin具有很高的相似性,约50%~70%;以邻接法(Neighbor-Joining,NJ)所构建的系统关系表明家蝇的Attacin与其他双翅目昆虫的Attacin起源于共同的祖先。

In this research, we investigated the edaphon community structure from Gaofeng forest-farm Eucalyptus plantation in Guangxi Nanning by PCR-RFLP technique and 16S rDNA sequence analysis.

本研究利用限制性内切酶片段长度多态性和16SrDNA序列分析相结合的方法研究了广西南宁高峰林场人工桉树林中土壤微生物的群落结构。

The successive stability constants which are obtained by non-linear least-squares methord fitting Bjerrum formula. In three- system ,the results from Scatchard plots indicate that there exists two strong binding site of Mn and one strong binding site of Zn,there are same results compareing with two -system ,but some more weak binding sites are different from the different addition sequence ,show that there are not common strong binding site of Mn,Zn in HSA or BSA .Strong binding sites have electiveness and unfalteringness ,weak binding sites exists irreversibility.

用非线性最小二乘法拟合Bjerrum方程,计算了同时竞争体系的逐级稳定常数;Scatchard图分析表明,三元体系中,Mn和Zn与血清白蛋白结强合部位数分别为2和1,与二元体系相比,保持不变,弱结合部位数随加入顺序的不同而不同,但都有所增加,竞争结果说明了Mn和Zn与血清白蛋白结合没有共同的强结合部位,强结合部位具有专一性,选择性,而弱结合部位具有不可逆性。

In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.

为研发防治乳牛子宫内膜炎的抗菌肽生物制剂,根据已发表的牛抗菌肽基因序列设计了1对引物,用试剂盒提取乳牛新鲜子宫内膜总RNA,经RT-PCR扩增,将克隆的抗菌肽BNBD5基因全长编码区的cDNA连接到原核表达载体pET30a中,经IPTG诱导原核表达,通过Western-blot检测融合蛋白BNBD5的表达水平。

In this paper, PCR primers were designed based on the sequence of a single copy in atp6 gene. A 500bp fragment was amplified, and then the PCR products were digested with restriction enzym BglII.

根据atp6的基因序列设计特异扩增引物,以瓣化型胡萝卜核质互作雄性不育系H05A及其保持系H05B为试材,扩增获得了1条约500bp的特异扩增片段。

Results Constituent ratio of pathogens in male genitourinary infection was Uu, Ng, Mh, eumycetes, Ct and Trichomonas in descending sequence.

衣原体抗原采用胶体金标记法,支原体培养采用郑州博赛性病支原体培养鉴定药敏试剂盒,结果判读按试剂盒说明书操作。

In additional,the content of GATA in the upstream sequence was very high.

使用同样方法,我们从球孢白僵菌中克隆出了完整的热休克蛋白HSP70基因编码区序列及上游序列。

The 16S rDNA sequence of the 4 strains were aligned with the 16S rDNA sequences of other species in genus Burkholderia,and the polygenetic tree was produced by using a Clustal V and treeview software.The results showed that P.cocovenenans subsp.farinofermentans was in a high homology with Burkholderia gladioli and Burkholderia cocovenenans, and they formed an independent phylogenetic clustal of genus Burkholderia.Key words: P.cocovenenans subsp.farinofermentans,16S rDNA sequcence,phylogeny

树状图及不同菌种之间的同源性比值:椰酵假单胞菌(P.cocovenenans subsp.farinofermentans)与唐菖蒲伯克霍尔德氏菌、椰毒伯克霍尔德氏菌的亲缘关系非常近,与其它菌种的亲缘关系相对较远,椰酵假单胞菌、唐菖蒲伯克霍尔德氏菌和椰毒伯克霍尔德氏菌可归属于一个独立的系统发育系。

Taking for example the salt rhythms of the Qianjiang Formation in the Qianjiang Sag of the Jianghan Basin (which is a typical Paleogene saline lacustrine basin in eastern China), through a detailed study on cores, the authors have determined for the first time the Ⅳ-order salt rhythm based on Ⅰ-,Ⅱ-and Ⅲ-order salt rhythms which were determined previously and got to know that the sedimentary process following the sequence of desalinizing→salinizing and crystallization of salt minerals from halite rock→(mud-bearing) glauberite rock→dolomite-bearing mudstone (mud-bearing doloston)→mudstone→doloston→glauberite rock→halite rock. The authors also analyzed the Ⅳ-order salt rhythm and the correspondence between its sedimentary records and the fluctuation of the palaeosalinity of waters and the short-scale (0.05~1.0 ka duration) change of dry-moist palaeoclimate.

本文以我国东部独具特色的古近纪古盐湖盆地-江汉盆地的潜江凹陷潜江组盐韵律为例,通过对王平1、王云10-6、王80-2等3口井连续取心段的精细研究,在前人划分Ⅰ、Ⅱ、Ⅲ级盐韵律的基础上,首次划分出组成含盐层系基础韵律单元-Ⅳ级盐韵律,弄清了它的沉积过程基本遵循从石盐岩→钙芒硝岩→含白云石泥岩→泥岩→白云岩→钙芒硝岩→石盐岩的淡化-咸化序列和盐类矿物的析出顺序;解析了Ⅳ级盐韵律及其沉积组合记录与水体古盐度波动和短尺度(0.05~1.0 ka)古气候干湿变化之间的对应关系。

Because of the rice genome sequence analysis, have access to a large number of human genetic information and function of rice genes, in order to cultivate high-yield, high-quality, delicious rice varieties lay the foundation for and contribute to understanding of wheat, corn and other gramineous crops Genome in order to bring the whole basis of food crops and applied research.

因为通过对水稻基因组进行全序列分析,人类能获得大量的水稻遗传信息和功能基因,为培育高产、优质、美味的水稻品种打下基础,并有助于了解小麦、玉米等其他禾本科作物的基因组,从而带动整个粮食作物的基础与应用研究。

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Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严,以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆,把帆卷起,系住。

Therefore, positively advances the interest rate marketability reform is one of current our country finance reform important tasks.

因此,积极推进利率市场化改革是当前我国金融改革的重要任务之一。