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We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

Deduction of the relationship between the boundary of buffer and Minkovski curve. A concept --- buffer curve --- is concluded from Minkovski curve and provide a way to unify the creation of all types of buffer on the same theoretical base.

12，导出缓冲区的边界和Minkovski曲线之间的关系，并从Minkovski曲线简化归纳出缓冲曲线的概念，将所有的缓冲区，包括单侧，双侧缓冲区的生成都统一建立在缓冲曲线的基础上。

The paper analyses the working process and the principle of undeland buffer circuit ,introduces the calculating method of parameter of buffer circuit ,and proves the effect of the circuit buffer by simulation.

分析了Undeland 缓冲电路的工作过程及原理，介绍了缓冲电路参数计算方法，最后用仿真的方法验证了电路的缓冲效果。

The effect of buffer control on user-level QoS having been studied in-depth with theoretical analysis. The buffer size providing determinate delay and delay jitter guarantee is found and the buffer size providing the optimal user-level QoS for a certain network condition is demonstrated.

从理论上深入挖掘缓存控制对用户层QoS 参数的作用，给出了提供确定时延和时延抖动保障的缓存容量值，论证了在网络环境一定时存在提供最佳用户层QoS 的缓存容量值。

A simple practical solution to this problem is to add a sequence number to our buffer class, and process the data in the buffer if the buffer sequence number is in order.

一个简单的实际解决这一问题的办法是增加一个序列编号，以我们的缓冲级，并过程中的数据缓冲区，如果缓冲区的序列号是在命令。

Through analyzing the theory and types of buffer overflow vulnerabilities, the format defines of the testing object are brought up, the manipulation on buffer and the status of buffer are defined.

通过对缓冲区溢出原理和各种缓冲区溢出类型的分析，对测试对象进行了形式化定义，并定义了缓冲区状态和缓冲区上的操作。

Taking the electric power design institutes over the country as firm technological support,our Company is the specialized manufacturer specializing in material transport products such link electric drive pusher series,aligning roller series,groov eelectric drive plough,wiper,electric drive anti-sticking T-cock,buffer air lock of new generation,electric drive buffer roll,buffer device,double-arm feeder,coal-covered curtain,gathering sill,belt head retractor,sprinkler,winding irrigator and so on,which are widely used in domestic thermal power plant,colliery,harbor,chemical and metallurgical industry.

本公司以全国各电力设计院为坚强的技术作后盾，是专业生产电动推杆系列，调心托辊系列，槽角电动犁式卸料器，刮水器，电动防卡三通，新一代缓冲锁气器，电动缓冲滚筒，缓冲装置，双推杆给料机，挡煤帘，导料槽，皮带头部伸缩装置，洒水器，卷绕冲洗器等物料输送产品的专业生产厂家。广泛应用于国内火电厂、煤矿、港口、化工、冶金等行业。

Influence of pH of buffer, the temperature, time of hydrothermal treatment on yield of free oil and the quantity of the soluble mass extracted in the buffer and the law of changing of the composition of components in the enzyme hydrolysis system with the pH of buffer, the temperature, time of HTT were studied.

首先优化热处理工艺，考察浸泡液的pH值、热处理时间及热处理温度对清油提取率及缓冲液中可溶性固形物溶出量的影响，同时分析酶解体系各部分组成随缓冲液的pH值、热处理时间及热处理温度的变化规律，为探索水酶法提油工艺的机理创造条件。

According to the short overwind distance characters of Baishan mine well,We adopt two stage buffer theory solving overwind buffer and falling prevention problem,put forward an effective method to slove the very_short_distance overwind buffer and falling prevetion problem that some old well exist commonly in our country.

针对百善矿主井过卷缓冲距离小的特点，采用二级缓冲理论解决过卷与防坠问题，为解决我国老井普遍存在的超短过卷距离过卷缓冲与防坠问题提供了一种有效方法。

The controller uses the data write buffer to reduce the data access waiting time, and employs the two ways of duo channels instruction pre-fetch buffer to decrease the fetching instruction waiting time and increase the instruction pre-fetch buffer hit rate. Meanwhile, to reduce the waiting time aroused by page miss of SDRAM, a four ways of on chip stack memory is introduced. Compared with traditional controller through experiments the novel SDRAM controller gets a high reduction up to 63% in access waiting time and 64% in page miss.

该控制器采用数据写缓存方式降低了数据在存取内存时的等待时间；并引入了两组双通道预取指令缓冲器，每组双通道都用以减少取指令时的等待时间，采用两组的结构是为了增加指令预取的命中率；同时还使用了四路组关联的片上堆栈存储器来降低SDRAM的页失效频率，从而降低了因页失效而需要等待的时钟周期。

Fostering the concept of multi-culture firmly and mastering the development of advanced culture correctly are conductive to resisting terrorism, separatism of nationalities and ultraism of religion, which is of great academic theoretical, immediate and profound historical si...

牢固树立多元文化的理念和正确把握先进文化的发展方向，有助于在世界范围内自觉地抵制恐怖主义，民族分裂主义和宗教极端主义。不仅具有重大的学术理论意义，而且也具有重大的现实意义和深远的历史意义。

But if anyone walks in the night, he stumbles, because the light is not in him.

11：10 若在黑夜行走，就必碰跌，因为那光不在他里面。