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The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养，22小时后上述各组卵母细胞的核成熟率分别为75.2％、73.1％、69.8％、63.5％，处于第一次减数分裂末期的比率分别为16.3％、15.9％、16.9％、27.0％，在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P＜0.05)，处于末期的比例明显低于其它各组(P＜0.05)：添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6％、66.6％、73.6％)明显高于BSA组的正常率43.3％(P＜0.05)；各组CG发生迁移较好的卵母细胞的比率分别为58.8％、33.9％、54.7％、47.9％，血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P＜0.05)。

PR content of the mixed type was higher than that of the hyperplastic t ype; and the PR content of the hyperplastic type was higher than that of the atr ophic type. The relationship between ER and PR contents of the vulvar dystrophy and adjacent normal tissue was in positive correlation. The PR content of vulvar squamous cell carcinoma went down along with the drop of its dedifferentiation degree. The PR content of carcinoma was much lower than that of the adjacent nor mal tissue.

结果：ER的含量混合型高于萎缩型，PR的含量混合型高于增生型，增生型高于萎缩型（ P值均＜0.05）；外阴营养不良及其邻近正常组织的ER与PR的含量之间呈正相关关系（P ＜0 。01），外阴鳞状细胞癌PR的含量随分化程度的降低而逐渐降低，且癌组织PR含量明显低于其邻近的正常组织。

Results:The serum sE-cadherin levels in health control did notvary throughout the menstrual cycle.The preoperative serum sE-cadherin levels were higher in patients with endometriosis than inhealth control.No differences were found between enometriosis patientswith differnt stage and clinical symptom.The preoperative serum sE-cadherin levels were higher in patients with ovarin cancer than in bothhealth control and patients with endometriosis.

研究结果：1、正常育龄妇女血清中sE-cad水平在月经周期中无明显改变〓，且血清中sE-cad水平与年龄不相关；2、内异症患者术前血清中sE-cad水平高于正常对照组〓，不同期别内异症患者血清中sE-cad水平无显著差别〓，有无痛经的内异症患者血清中sE-cad水平无显著差别〓；3、卵巢癌患者术前血清中sE-cad水平分别高于内异症患者组及健康对照组〓。

Both overlong or overshort bottom duration and overlong or overshort fluctuation period are unfavorable for reduction of non-diamond content. Only moderate ones have optimal effects on it.3 Compared with a smooth microwave, a fluctuating one can reduce the diamond nucleation density slightly.4 In fluctuating microwave, even if a higher CH〓 concentration chosen, diamond films with quality better than that of those deposited at a lower CH〓 concentration and in smooth microwave can be obtained.In smooth microwave, the influence of O〓 upon the diamond films＇MWPCVD was investigated. It is found that:1 In the range of lower O〓 concentration, its increase is beneficial to improvement of quality of diamond films, but in that of higher one, its increase conversely deteriorates the quality of diamond films.

在脉动微波下进行了沉积金刚石膜的研究，得到下述结果：1）采用合适的脉动微波，能使金刚石膜的沉积质量显著提高，并能使金刚石膜中的微晶石墨得到几乎完全的抑制。2）过长或过短的平底时间或脉动周期均不利于金刚石膜中非金刚石相碳含量的降低，只有适中的平底时间或脉动周期才有最佳的降低非金刚石相碳含量的效果。3）脉动微波会使金刚石的形核密度有所下降。4）脉动微波下，即使选用较高的CH〓浓度，也能得到沉积质量比在平稳微波下采用较低的CH〓浓度所制备的金刚石膜的沉积质量要明显提高的金刚石膜。

Higher nutrient availability could significantly advanced seedling survival rate and overwinter survival rate for some species, but no significant effect on seedling emergence rate.3 Middle light and higher nutrient microsite can significantly advanced seedling recruitment probability for these species.

高养分生境可以显著提高一些物种的幼苗存活率和越冬率，而对出土率没有显著影响。3中等光照下的高养分微生境可以显著提高这些物种的幼苗更新补充概率。

The number of forward sequences was higher than that of reverse ones in Bos taurus, but with similar genetic diversity. While the reverse sequence from Ovis aries had higher genetic diversity than forward ones.

在牛上，正向片段跟反向片段有较近的遗传距离，而在绵羊上正、反向片段的遗传距离较远。

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10％FBS，细胞浓度为100个／400μl，发育培养体系为NCSU-23+BSA；用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v／20μs／2次+600μMγ-BLI；以活力为0.1左右的冷冻精液进行IVF时，精子浓度为10~7，共孵时间为6h，并在成熟处理时只部分去除卵丘细胞；MⅡ卵母细胞冷冻时，方法为移液枪头法+离心法，冷冻后激活的2-细胞卵裂率比IVF高。

Results According to the treatment group, The total protein、albumin、globulin、the former albumin、serum glucose are higher than that of the control group; the Immunoglobulin (IgG、IgA、 IgM) are significantly higher than that of the control group, and the urea is significantly lower than that of the control group. Other indicators: total bilirubin、glutamic pyruvic transaminase、glutamic oxalacetic transaminase、alkaline phosphatase、creatinine、hemoglobin and the red blood cell count have no significant difference between the two groups.

结果 治疗组总蛋白、白蛋白、球蛋白、前白蛋白、血糖比对照组高；尿素比对照组明显减低；免疫球蛋白（IgG、IgA、IgM）比对照组明显升高；总胆红素、谷丙转氨酶、谷草转氨酶、碱性磷酸酶、肌酐、血红蛋白、红细胞计数等项，两组无明显改变。

The results support the idea that the temperature difference between the Palaeocene tropics and higher-latitude regions was as large as it is today, even though the higher latitudes were much warmer during that time.

这个结果与古新世和高纬度地区的气温差别相符。尽管那时纬度高的地方要相对暖和一些。

The content of essential amino acid and flavor amino acid of experiment groups were higher than control group, especially group 1.0% small peptide were increased by 6.4% and 2.5%; IMP content of experiment groups was increased in different extent, especially group 1.0% small peptide was 0.17mg/100mg and increased by 70.0%(P.05); The content of palmitoleic acid、 octadeca、 arachidonic、 arachidic and stearic acid of experiment groups were higher than control group indifferent extent.

随着小肽用量的增加，成鲤肌肉中的总氨基酸含量呈上升趋势，必须氨基酸和风味氨基酸的含量也随之提高，尤其是1.0％小肽组的必须氨基酸和风味氨基酸的含量分别比对照组提高6.4％和2.5％，其中谷氨酸的含量最高，为10.70mg／100mg，比对照组提高14.0％(P＜0.05)；随着饵料中小肽用量的增加，成鲤肌肉的肌苷酸含量显著提高(P＜0.05)，1.0％小肽组的IMP含量高达0.17mg／mg，比对照组提高70.0％；成鲤肌肉中的油酸和二十？

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。