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Objective To study the protective action of compound agent madeup with effective ingredients of Hirudo and Rhizoma chuanxiong and Folium Ginkgo in animal ischemic cerebral infarction.Methods To mix Hirudin extracts of Folium Ginkgo and Ligustrazine according2:25:5into asepsis injection,rats local cereˉbral ischemia induced by embolism of middle cerebral arterywas used as experimental model to study the efˉfects of the compound agent and positive control drug,Nimodipine and extracts of Folium Ginkgo on cerebral infarct size and neurological deficits.Disposing the experimental data with statisticsand comparing their otherness among difˉferent groups.

目的 探讨用中药水蛭、银杏、川芎的有效成分组成的复方制剂对动物局灶脑缺血的保护作用方法将水蛭素、银杏叶提取物、川芎嗪按2∶25∶5的比例制成无菌注射液，应用鼠大脑中动脉栓塞导致缺血性脑卒中的实验模型，并用生理盐水、尼莫地平和银杏提取物作对照，观察不同药物对脑缺血面积和由缺血造成的大鼠行为障碍的影响，将实验据用统计学处理并进行组间的差异性比较。

As shown,the antigen is a glycoprotein present on the surface of cells. Extracts from Hep G2 hepatocellular carcinoma cells, HT-29 colon cancer cells, SKHep-1 liver adenocarcinoma cells, BT-20 breast cancer cells as well as U937 histiocytic lymphoma cells were positive for the precipitation of Ag-Ab complex, which were later reactive with the anti-3A5 McAb staining. While extracts from normal human endothelial cells showed no precipitation in Western blot.

抗原分子量为63kDa，是一种存在于细胞膜上的糖蛋白，在肝细胞肝癌Hep G2细胞，肠癌HT-29细胞，肝管癌SK-Hep-1细胞，乳腺癌BT-20和人组织细胞淋巴瘤U937细胞膜提取物中，均有此蛋白带，并能被单抗3A5特异性地识别，而人正常内皮细胞则不见此反应带。

For anti-oxidation properties, methanolic extracts from fruit bodies and mycelia showed excellent scavenging ability toward DPPH (1,1-diphenyl-2-picryl hydrazyl)(96% at 15mg/mL) and good potential in reducing ability at the concentration of 2.5 mg/mL and chelating effect on ferrous ion (about 82% and 77% for extracts from mycelia and fruit bodies, respectively) at the concentraction of 2.5 mg/mL.

在抗氧化性质方面，结果显示子实体和菌丝体的甲醇萃取物在浓度为15mg/mL时，对DPPH（1，1-diphenyl-2-picryl hydrazyl）自由基清除率高达96﹪和89%，在浓度为2.5mg/mL时就具有好的还原能力，菌丝体和子实体甲醇萃取物在浓度於2.5 mg/mL时，对铁离子螯合能力分别达82﹪和77﹪。

Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.

基于以上两种重要疾病和研究组方向，本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作，累计筛选1万多个/次样品，发现10余个活性较强化合物；从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究，分离鉴定了58个化合物，发现6个新化合物和14个抗肿瘤活性化合物；通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究，发现它可能是通过降低G2期重要的转录因子E2F-1，致使细胞周期阻滞在G0/G1期，引起多发性骨髓瘤U266细胞凋亡；开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析，结果表明计算与实验一致。

II. The State practices the special-purpose control system with respect to the production, management, use and export of ephedrine (including extracts from ephedra grass and chemically synthesized, laevorotatory and dextrotatory) as well as their saline categories such as ephedrine hydrochloride, ephedrine oxalic, ephedrine sulphate and raw products of ephedrine (including ephedrine extracts, ephedrine extract powder and ephedra powder), ephedrine derivatives and prescribed preparations made with ephedrine as the raw material.

二、国家对麻黄素(含从麻黄草提取和化学合成的，包括左、右旋)及其盐类如盐酸麻黄素、草酸麻黄素、硫酸麻黄素和麻黄素粗品(含麻黄浸膏、麻黄浸膏粉、麻黄草粉)、麻黄素衍生物以及以麻黄素为原料生产的单方制剂等的生产、经营、使用、出口实行专项管理制度。

The State practices the special-purpose control system with respect to the production, management, use and export of ephedrine (including extracts from ephedra grass and chemically synthesized, laevorotatory and dextrotatory) as well as their saline categories such as ephedrine hydrochloride, ephedrine oxalic, ephedrine sulphate and raw products of ephedrine (including ephedrine extracts, ephedrine extract powder and ephedra powder), ephedrine derivatives and prescribed preparations made with ephedrine as the raw material.

二、国家对麻黄素(含从麻黄草提取和化学合成的，包括左、右旋)及其盐类如盐酸麻黄素、草酸麻黄素、硫酸麻黄素和麻黄素粗品(含麻黄浸膏、麻黄浸膏粉、麻黄草粉)、麻黄素衍生物以及以麻黄素为原料生产的单方制剂等的生产、经营、使用、出口实行专项管理制度。

The State practices the special-purpose control system with respect to the production, management, use and export of ep hedrine (including extracts from ep hedra grass and chemically synthesized, laevorotatory and dextrotatory) as well as their saline categories such as ep hedrine hydrochloride, ep hedrine oxalic, ep hedrine sulphate and raw products of ep hedrine (including ep hedrine extracts, ep hedrine extract powder and ep hedra powder), ep hedrine derivatives and prescribed pr ep arations made with ep

国家对麻黄素(含从麻黄草提取和化学合成的，包括左、右旋)及其盐类如盐酸麻黄素、草酸麻黄素、硫酸麻黄素和麻黄素粗品(含麻黄浸膏、麻黄浸膏粉、麻黄草粉)、麻黄素衍生物以及以麻黄素为原料生产的单方制剂等的生产、经营、使用、出口实行专项管理制度。

IC50 of these cells exposed to the extracts from N-butanol for 48 hours approach 7.94% and 10.72%. Conclusion The N-butanol extracts of Livistona chinesis had obvious anti-tumor activity in vitro.

蒲葵子提取物的各萃取部位能抑制肝癌细胞HepG2和白血病细胞HL60的增殖，有较强的体外抗肿瘤活性。

The results showed aqueous shoot extracts of Euphorbia maculata could inhibit seed germination speed in all the four species, reduce seed germination rate in Lycopersicon esculentum, Schizonepeta tenuifolia and Capsicum frutescents, and its effects increased with the increase of extracts concentration, while having no effects on seed germination of Edible umaranth.

结果表明，斑地锦浸提液可降低四种蔬菜种子的发芽速率，抑制番茄、辣椒和荆芥的发芽率，并且这种抑制作用随处理浓度的提高而加强，而对苋菜种子的发芽率无显著影响；0.4 g/ml的斑地锦浸提液强烈抑制了四种蔬菜根的生长，却不同程度地促进了辣椒、荆芥和苋菜茎的生长，对根/茎比的影响与对种子发芽率的影响类似。

In conclusion, Macleaya cordata extracts improved growth performance and alleviated weaning stress in weanling piglets. This study demonstrates the potential use of Macleaya cordata extracts as a feed additive of weaned piglets instead of antibiotics.

试验显示，血根碱和博落回提取物均能显著提高断奶仔猪的生长性能，有效缓解了断奶应激，血根碱或博落回提取物作为植物源性抗生素替代抗产品在断奶仔猪日粮中有良好的应用效果。

At the rear was an innovative combination transaxle with the gearbox, clutch, differential, and inboard-mounted drum brakes.

在后面是与变速箱，离合器，差的创新结合变速，和内侧安装鼓式制动器。

For more people can apprecaite and have the master work, there is reproduction painting appears in the market, the large various selection of paint,the high saturation of color plus the plentiful sfumato, to make the reproduction perfect copy and detail as original painting, so as to meet the requirment of amateurs who love art paintings,they do not need to pay high price to get classical paintings.

颜料色域广，色彩饱和度高，色彩层次丰富，图像色彩还原真实，能完美的复制原作，满足艺术爱好者欣赏，艺术爱好者们不用支付昂贵的价格就能拥有经典油画。