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MethodsIsolated uterus preparations of above animals were made according to the literature and its contractive effects to four different extracts of Phrynium capitatum Willd flower were observed and recorded with BL-410 experimental system for biological function; the EC50s of the extracts were determined and compared with it of Herba Leonuri fluid extract.

目的探讨棕叶花不同提取物对家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用。方法采用BL-410生物机能实验系统,观察棕叶花4种提取物对非孕或注射己烯雌酚后家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用,并用EC50与益母草流浸膏进行比较。

MethodsIsolated uterus preparations of above animals were made according to the literature and its contractive effects to four different extracts of Phrynium capitatum Willd flower were observed and recorded with BL-410 experimental system for biological function; the EC50s of the extracts were determined and compared with it of Herba Leonuri fluid extract.

方法采用BL-410生物机能实验系统,观察棕叶花4种提取物对非孕或注射己烯雌酚后家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用,并用EC50与益母草流浸膏进行比较。

Purified water, VP/VA copolymer, vegetable glycerin, hydrolyzed wheat protein, biotin, panthenol, niacin, extracts of camellia sinensis and serenoa serrulata, certified organic extracts of salvia officinalis, aloe barbadensis leaf, lavandula angustifolia, melissa officinalis, calendula officinalis and chamomilla recutita, sodium hydroxide, carbomer, phenoxyethanol.

纯水、VP / VA共聚物、蔬菜甘油、水解小麦蛋白、素、泛醇、烟酸、提取物的茶树和serenoa serrulata,经认证的有机液(鼠尾草、琴柱草酝酿索芦荟叶片,)小甜甜沙枣淡紫色,酝酿和chamomilla金盏花酝酿recutita(matricaria、氢氧化钠、萃取。所有发质都适合。

MethodThe extracts was prepared by grinding 800-1 000 mg fresh leaves of Clerodendron inerme and Vitex rotundifolia added with liquid nitrogen. The sample of total RNA was prepared by centrifugating the extracts added with reagent at 4 ℃, centrifugating at room temperature after ice bath and centrifugating at room temperature after adding reagent in order.

方法]取800~1 000 mg许树和单叶蔓荆的鲜叶,加液氮研磨后制成提取液,提取液加试剂4 ℃下离心,冰浴后室温下离心,依次添加试剂后室温下离心,即得total RNA样品。

2Ai. In addition, cell extracts from E. coli JM109(DE3) cells harbouring the recombinant plasmid pAM28 were able to decarboxylate the histidine present in the reaction to histamine, whereas extracts prepared from control cells containing the vector plasmid alone did not.

此外,从大肠杆菌细胞提取物大肠杆菌JM109中(DE3)中细胞重组质粒pAM28窝藏能够decarboxylate在本组氨酸为组胺反应,从控制细胞制备含有载体质粒提取物,而仅仅没有。

We are always concerned about the international market for natural herbal products to changes in demand, research and development, production Epimedii, osthol, Diosmin, Ginkgo biloba, lycopene, blueberry, green tea and other plants more than 30 kinds of standard extracts, we have and will continue its efforts to develop all the useful people healthy plant extracts new products.

我们时刻关注着国际市场上人们对天然草药产品不断的需求变化,研究开发生产淫羊藿、蛇床子、地奥司明、银杏叶、、番茄红素、越橘、绿茶等30 多种标准植物提取物,我们已经且将会继续努力开发出一切有益人们身体健康的植物提取新产品。

Results: the extracts of dipsacus asperoids with different concentration(1ug/ml、 10ug/mk、 100ug/ml) can increase the ability of osteoblastic proliferation; the extracts of dipsacus asperoids with concentration of 10ug/ml and 100ug/ml can increase the ability of osteoblastic differentiation.

结果:浓度为1ug/ml、10ug/ml和100ug/ml的续断甙溶液有促进成骨细胞增殖的作用;浓度为10ug/ml和100ug/ml的续断甙溶液有促进成骨细胞ALP分泌的作用。

Results: the results showed that extracts of Artemsia dracunculus L. at the dose of 7.5%,3.75%,1.875% 20 ml/kg.w, can cause a signficant increase of WBC (P.05), thymus index, spleen index,(P.05) phagocytic rate and phagocytic index of phagocyte,(P.05) the transformation rate of the spleen lymphocytes,(P.05) and the serum IL-2(P.05),serum hemolysin concentration 50%( P.05)in immunodepressed mice. Conclusions: extracts oil from Artemsia dracunculus L. can significantly enhance the non-specific, cell-mediated and humoral immunological function in immunodepressed mice.

结果:与模型组比较,椒蒿挥发油各组均能升高小鼠外周血白细胞数(P<0.05);均能升高胸腺指数(P<0.05)、脾指数(P<0.05);均能提高免疫受抑小鼠腹腔巨噬细胞的吞噬率,吞噬指数(P<0.05);均能升高脾淋巴细胞转化率(P<0.05);椒蒿挥发油各组血清IL-2值明显高于环磷酰胺对照组(P<0.05);椒蒿挥发油各组均可升高小鼠的血清半数溶血值(P<0.05)。

The fungicidal activity of methanol extract was higher than that of ethylacetate extract. The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Botrytis cinerea, Alternaria longipes, Glomerella cingulata, Curvulavia lunata, Gibberella zeae, Valsa malt under the concentration of 4000μg/mL, the inhibiting ratio against pathogen growth was ranging from 62.9%~85.1%; The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, the EC50 values was 62.5328μg/mL; The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Bacillus cereus , Bacillus subtilis.

其中甲醇提取物的抑菌活性较乙酸乙酯好,在4000μg/mL浓度下对番茄灰霉病菌、烟草赤星病菌、苹果炭疽病菌、玉米弯孢叫斑病菌、小麦赤霉病菌、苹果腐烂病菌6种植物病原真菌均有抑制作用,抑制率在62.9%~85.1%;抑制孢子萌发试验表明:菌丝体甲醇提取物对烟草赤星病菌的毒力较好,其EC50仅为62.5328μg/mL;抑制细菌活性表明菌丝体甲醇提取物对蜡状芽孢杆菌和枯草芽孢杆菌有一定的抑制作用,而对大肠杆菌无明显的抑制作用。

The results indicated that the water or 50% ethanol extracts from peonies petals could reduce light absorption of DPPH and OH initiated hydroxylation of salicylate, and inhibit O2(superscript -) mediated light chemical reduction of nitroblue tetrazolium effectively, and lipid peroxidation of polyunsaturated fatty from yolk lipoprotein, in which DPPH scavenging activities of 6 peonies water extracts were hinger than that of 200 μg/mL BHT, but lower than 80 μg/mL Vc. In 4 kinds of detecting systems of antioxidation, the peroxidation activity of deep colored flowers of peonies was higher than that of light colored flowers of peonies.

牡丹花瓣提取液能明显抑制卵黄组织匀浆的脂质过氧化作用,降低DPPH的吸光值、抑制O2介导的NBT光化学还原及OH作用下的水杨酸羟基化作用,其中牡丹花瓣提取液清除DPPH自由基的活性高于200μg/mL BHT,但低于80μg/mL Vc.6个品种的牡丹花瓣提取液,在4种测定系统中均具有一定程度的抗氧化能力,且水提取液的抗氧化活性略高于体积分数50%乙醇提取液,深色花瓣提取液对不同自由基的清除能力较强。

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