查询词典 expression error

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Results: In contrast to negative expression in normal oral mucosa, survivin was expressed in 42 of 50 (84%) cases of oral squamous cell carcinoma samples. There was no significant correlation between survivin expression and age and sex. In squamous cell carcinoma tissues, the expression of Survivin gene stepped up following malignant degree, but the positive rate of Survivin was not statistically significant.

结果：Survivin蛋白在10例正常口腔粘膜组织中呈阴性，而在50例口腔鳞癌组织中有42例阳性，占84%，差异有极显著性(P.01)；COX-2蛋白在10例正常口腔粘膜组织中呈阴性，但在口腔鳞癌组织中的阳性率为86%(43/50)，差异有极显著性(P.01)；Survivin蛋白阳性率与年龄和性别无相关性，而与COX-2蛋白表达阳性呈正相关(P.05)。

Expression patterns of myosin light Chain 2 in moth period of Bombyx mandarina and Bombyx moriThe result of expression pattern of MLC2 gene in silkworm moth and wild silkworm moth by Real-Time Quantitative PCR showed that there was no significant difference in expression of MLC2 gene in silkworm and wild silkworm.

家蚕和野桑蚕蛾期MLC2基因的表达模式用实时定量PCR分析家蚕和野桑蚕MLC2基因在蛾期的表达模式，结果显示：MLC2基因在家蚕与野桑蚕中的表达没有差异。

In situs hybridization histochemical technique is applied to detect the expression of five subtype of human somatostatin receptor Mrna (hSSTR1-5), including the expression model of 54 cases of various tumor specimen and the expression density of 24 lung cancer cases.

本研究采用原位杂交组织化学技术，探测高度表达人生长激素抑制素受体的5种亚型(hSSTR1-5)的54例肿瘤标本，包括54例肿瘤SSTR亚型的表达模式及其中24例肺癌的表达密度。

Results Analysis of the expression of proteins of JAK2 in leukemic blasts, there was no detectable constitutive expression of phosphorylated JAK2 in all the AML samples analyzed; Furthermore, we could detect a constitutive activation of STAT 3 by immunoprecipitation of the targeted protein in primary leukemic blasts from 3 of 11 AML patients without any cytokine treatment; Pretreatment clinical characteristics, and treatment regimens did not differ significantly between patients with and without constitutive STAT3 activity, there was not significant relation between the constitutive activation of STAT3 and the FAB subvariety and chromosome karyotype; However, the 3 patients with constitutive STAT3 activity seemed to have adverse treatment outcome, one did not show remission, the other two patients relapsed shortly after remission; Consistent with the above-mentioned result, leukemic blasts from the 3 samples with constitutive expression of STAT3 showed higher percentage of spontaneous apoptosis and S-phase cells.

在实验研究基础上，本研究进一步运用免疫沉淀和Western Blot印迹方法，检测AML患者原代细胞JAK2和STAT3活化情况，并分析了STAT3活化与AML患者预后有关指标改变的关系。结果我们的初步研究发现，11例AML患者中，有3例呈现STAT3组成型活化（STAT3阳性，下同），无一例呈JAK2组成型活化。上述3例STAT3组成型活化与FAB分型和染色体核型改变未见明显相关性；在此3例患者中，1例未取得缓解，另外2例虽然取得短暂缓解，但均在二个月内复发。上述3例STAT3阳性AML患者S期白血病细胞百分率数值较组高，而凋亡率数值较低。

Therefore, NAG7 gene was a nice candidate of tumor suppresser gene associated with NPC. The mechanism might be that the re-expression of NAG7 gene contributed to the change of gene expression profile and following the change of protein expression profile, then involved in the cell cycle and apoptotic regulation changed, and finally suppressed the NPC growth.

因此，NAG7基因作为鼻咽癌相关的抑瘤基因候选者，其作用机理可能是通过诱发基因表达谱的改变而引起蛋白质表达谱发生相应的改变，在一致性改变的功能分子的作用下，抑制细胞增殖，同时诱发细胞凋亡增加，从而发挥抑制鼻咽癌细胞生长的作用。

The results of SDS-PAGE showed that the expression products were about 31KD and reached their expression peaks about 2 hours after inducing and the expression quatity of fusion protein had 22.7% ratio of total thalline protein, while the E.

提取并纯化pET-PIL-8的融合表达蛋白并用改进的琼脂扩散法检测其生物学活性，通过检验分析表明所获得的重组猪IL-8具有趋化猪外周血淋巴细胞的作用，具有较强的生物学活性。

After amputation, the expression of desmin appears first in the distal end epidermis and the peritoneum 4 d PA. The expression in the peritoneum lasts to the 14 d PA when expression of desmin appears in the musculature, while that in the distal end epidermis only lasts to the 8 d PA when the connective tissue below begins to express desmin. Moreover, from the 4 d PA, musculature and epidermis of the tube foot begin to express desmin and the expressions last to the 20 d PA. From 8-14 d PA, musculature of the ambulacral plate expresses myogenin and the peritoneum also expresses myogenin from 14-20 d PA. All these results indicate that precursor cells for muscle regeneration in adult starfish A.rollestoni are mainly from the distal end epidermis and the parietal peritoneum.

创伤后第4 d，顶端表皮层及体腔上皮中首先出现了desmin表达，体腔上皮中desmin的表达一直持续到第14 d，此时其上方的肌肉组织中也出现了desmin表达；而顶端表皮层中desmin的表达仅持续到第8 d，同时其下方的结缔组织也开始表达desmin；此外，从第4 d起，管足肌肉及管足表皮层开始表达myogenin，且该表达一直持续到创伤后的第20 d；而第8 d起，步带板肌肉也出现了myogenin的表达，此表达一直持续到第14 d；从第14 d起，体腔上皮也出现myogenin表达，且一直持续到第20 d。

Include: Of history of civilization of baleful and misinterpret China, China and historical historical fact; Baleful and misinterpret his country the history, do not respect what civilization and custom are used to human civilization, his country; Derogate in cold blood, evil of figure of the anybody in doing famous book of character of revolutionary cacique, hero, important history character, China and foreign countries and famous work; Ill will derogates people army, armed police, public security and judicatory figure; Have mistreat captive, inquisition by torture to extort a confession of the clue such as convict or guilty suspect; Expression violates guilty aggressive arrogance, specific reveal criminality detail, reveal special investigation shift, expose what ought to be protected to inform against the figure such as person, witness, audio; Advertise religious ultraism, instigate between each religion, denomination, the contradiction between be religious and masses of lack of faith and conflict, harm masses emotive; Advocate sees photograph, fortune-telling, see ghost of geomantic, augur, drive treat a disease those who wait for feudal superstition activity; With evil do means to depict heavy nature calamity, contingency, horrible incident, war to wait for catastrophic occasion; Specific show licentious, rape, incestuous, love cadaver, walk the street, go whoring, of the clue such as sexual allergy, masturbation; Of the detail such as means of process of expression or obscure performance sexual behavior, sex, sex and as associated as this overmuch body contact; Show intentionally, be masked with limbs only or cover position of human body privacy with very small pall; Contain sexual suggestion, sex to tease those who wait to make the person produces a gender to associate easily; Advocate extramarital love, much horn is loved, one-night standing, sex is mistreated and change wife wait for insalubrious content; In order to grow up the film, affection lubricious film, 3 class piece, pat secretly, expose one's body, dew point reachs all sorts of flirtation to sexual written language or picture regard video program as caption or be classified; Have intense of the clue such as excitant homicide, bloody, violent, suicide, kidnap, drug taking, gamble, clever different; Have picture of excessive fright bloodcurdling, caption, background music and sound effect; Specific show animal of cause sb's death by maltreating him, catch kill, edible country protects kind of animal; Contain those who encroach individual privacy content; With affirmative, favorable fundamental key or fetching me-too means expression fights other people of affray, shame, corrupt of character bawdry; The philosophy with inactive, decadent advocate, world outlook and viewpoint of value, unwisdom of sedulous apply colours to a drawing, exaggerated nation lags behind or of social the seamy side; The cutout of the program of movie and TV that total bureau of TV of national broadcast film prohibits travelling and film, teleplay reduces part; Violate relevant law, statute of spirit.

包括：恶意曲解中华文明、中国历史和历史史实的；恶意曲解他国历史，不尊重人类文明、他国文明和风俗习惯的；蓄意贬损、恶搞革命领袖、英雄人物、重要历史人物、中外名著及名著中重要人物形象的；恶意贬损人民军队、武装警察、公安和司法形象的；有虐待俘虏、刑讯逼供罪犯或犯罪嫌疑人等情节的；表现违法犯罪嚣张气焰，具体展示犯罪行为细节，暴露特殊侦查手段，暴露应当受到保护的举报人、证人等形象、声音的；鼓吹宗教极端主义，挑起各宗教、教派之间，信教与不信教群众之间的矛盾和冲突，伤害群众感情的；宣扬看相、算命、看风水、占卜、驱鬼治病等封建迷信活动的；以恶搞方式描绘重大自然灾害、意外事故、恐怖事件、战争等灾难场面的；具体展现淫乱、强奸、乱伦、恋尸、卖淫、嫖娼、性变态、自慰等情节的；表现或隐晦表现性行为、性过程、性方式及与此关联的过多肉体接触等细节的；故意展现、仅用肢体掩盖或用很小的遮盖物掩盖人体隐私部位的；带有性暗示、性挑逗等易使人产生性联想的；宣扬婚外恋、多角恋、一夜情、性虐待和换妻等不健康内容的；以成人电影、情色电影、三级片、偷拍、走光、露点及各种挑逗性文字或图片作为视频节目标题或分类的；有强烈刺激性的凶杀、血腥、暴力、自杀、绑架、吸毒、赌博、灵异等情节的；有过度惊吓恐怖的画面、字幕、背景音乐及声音效果的；具体展示虐杀动物，捕杀、食用国家保护类动物的；带有侵犯个人隐私内容的；以肯定、赞许的基调或引人模仿的方式表现打架斗殴、羞辱他人、污言秽语的；宣扬消极、颓废的人生观、世界观和价值观，刻意渲染、夸大民族愚昧落后或社会阴暗面的；国家广播电影电视总局禁止传播的影视节目以及电影、电视剧的删减片段；违反相关法律、法规精神的。

Many will continue to choose to live in duality and in conflict.

许多人将继续选择活在二元对立性和冲突中。

I find that students of the University of Physical Education all wear sportswear at first sight.

我发现：体育大学的学生乍一看，都是穿运动衣，大家都一样