查询词典 expressing

Our previously study has been showed that transgenic N.benthamiana and N.tabacum plants expressing TYLCCNV DNAβC1 developed pleiotropic morphologic abnormalities such as leaf curling,distortion and interveinal protuberances on the abaxial surface. To determine whether theβC1 protein of TbCSY is also responsible for symptom induction,we prepared transgenic N.benthamiana and tomato plants expressing the TbCSV DNAββC1.Of the rooted plantlets,only 10%of transgenic N.benthamiana plants were moderately abnormal,including upward leaf curling.

DNAβ互补链上编码一个保守的ORF，即βC1，对TYLCCNV DNAβ的βC1的突变发现它编码一个症状决定因子，表达TYLCCNV DNAβ的βC1的转基因烟草产生曲叶、畸形和叶背面组织增生等发育异常的表型，而大部分转化TbCSV DNAβ的βC1基因的烟草和番茄植株均与正常植株类似，只有少数烟草表现出轻微叶上卷表型。

Mixtures of the final whole cultures of recombinant strains Bt B601, Bt B611, Bt B640, Bt U 30(expressing Cry4Aa, Cry4Ba, Cry11Aa and Cyt1Aa protein of Bacillus thurigiensis subsp. israelensis respectively) and Bt CW 3(expressing the Binary toxin of Bacillus sphaericus ) were used in bioassays against resistant Cpq larvae to value the synergism among the mosquito larvicidal toxins.

本研究测定了分别表达苏云金芽孢杆菌Cry4Aa、Cry4Ba、Cry11Aa、Cyt1Aa和球形芽孢杆菌二元毒素Bin的转化菌株Bt B60 1、Bt B611、Bt B640、Bt U 30和Bt CW 3全发酵培养物两两或两两以上不同组合对抗性库蚊的毒力，分析了杀蚊毒素间的协同作用。

These ES cells were introduced into host embryos from outbred KMW though blastocyst injection. Using GFP, the attractive genetic marker, we have been able to follow the fate of ES cells in living embryos and to observe directly the contribution of these cells to mouse embryos. Totally, four GFP-expressing 'green' chimeric mice expressing GFP were obtained.

为研究嵌合体动物中供体胚胎干细胞在宿主胚胎发育中的走向和定位，同时探讨绿色荧光蛋白基因作为报告基因在转基因动物制作中的应用价值，本研究将pEGFP-N1基因导入小鼠ES-D3细胞系，得到稳定表达GFP的胚胎干细胞亚系ES-D3-GFP，通过对昆明小鼠的囊胚腔注射，获得了4只表达绿色荧光蛋白的嵌合体小鼠。

The invention discloses a method for expressing polyhydroxy fatty acid ester and a specific project bacterial, wherein the project bacterial for expressing polyhydroxy fatty acid ester is the mutant of the polyhydroxy fatty acid ester bacterial by inhibiting or removing a or a plurality genes related to fatty acid beta oxidization metabolic pathway in the polyhydroxy fatty acid ester bacterial.

本发明公开了聚羟基脂肪酸酯的一种表达方法及其专用工程菌。该用于表达聚羟基脂肪酸酯的工程菌是抑制或敲除产聚羟基脂肪酸酯细菌中的与脂肪酸β氧化代谢途径相关的一个或多个基因后得到的产聚羟基脂肪酸酯菌突变株。

Notably,we also found that anti-CTLA4 can wellsynergize with B7-expressing tumor vaccines.This result is different with that ofHurwitz et al obtained from a similar research in 1998,in which they found thatanti-CTLA4 can not synergize with B7-expressing tumor vaccines.

值得注意的是，在我们的实验中发现anti-muCTLA4抗体与B7瘤苗联合治疗小鼠肿瘤具有很好的协同效应，这与1998年Hurwitz等在类似的研究中所得出的结论不同，即他们认为anti-muCTLA4抗体与B7瘤苗间没有协同抗小鼠肿瘤作用。

The unspliced and spliced forms of XBP1 stably expressing NS4B in HeLa cells, the transcriptional levels of ATF6, Grp78 and caspase-12, and the luciferase activity in XBP1 and Grp78 promoter reporter assays in HeLa and Huh-7 cells expressing NS4B were detected.

用 RT-PCR 和免疫印迹的方法检测稳定表达 NS4B 的 HeLa 细胞中的 XBP1；通过 RT-PCR 的方法在表达 NS4B 的 HeLa 和 Huh-7 细胞中检测 ATF6，Grp78 和 caspase-12 的转录，并且通过报告基因的方法分析 XBP1 和 Grp78 启动子活性。

The transgenic lines expressing sense RNA reduced PLRV titers about 43%～72% in comparison with untransformed plants, while the transgenic lines expressing antisense RNA reduced about 72%～86%. It showed that the antisense RNA confered higher resistance to PLRV.

表达正意RNA的转基因植株PLRV滴度降低43%～72%，表达反意RNA的转基因植株PLRV滴度降低72%～86%，由此可见，表达PLRV IS反意RNA的转基因马铃薯对PLRV抗性较强。

In vivo time-lapse images showed that ICL-expressing neurons have more sparsely branched dendritic arbors, which expand over larger neuropil areas than EGFP-expressing control neurons.

活细胞长期观察实验证明，EGFP-ICL细胞的树突总面积比对照组增大，而树突的分枝减少。

Singer realizes the whole adjustments by way of breathing movements; sound-producing movements; vibration movements; language movements; oral cavity movements; emotion-expressing movements so as to improve the expressing ability of vocal musical art .

本文通过歌唱的呼吸运动；发声运动；共鸣运动；语言运动；行腔运动；抒情运动进行整体的调控，以提高歌唱艺术的表现力。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N 末端带上含24 bp的flag标签，克隆到pGEM T easy载体并测序，再亚克隆至真核表达载体pcDNA3 1，酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞，Western blot检测flag cbl在细胞中的表达。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。