查询词典 expressing

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The second chapter is on the category of pragmatic quantity which are expressed by modality, Three "shi" structures expressing pragmatic quantity are discussed: the adverbs of degree "henshi\',"zuishi" structures expressing subjectivity; the rhetoric question structure of "bushi" expressing different kinds and scales of pragmatic quantity; and the compound sentence structure of "bushi不是 A, jiushi B expressing emphasizing.

着重考察了三种表达语用量的&是&字结构：能进行主观表达的程度副词&很是、最是&、能表达语用量的不同类型和等次的特定句型&不是&型反问句、能表达强调功能的&不是A，就是B&复句格式。

The strategies used for indirectness recognition are generalized in terms of false expressing, uninformative expressing, expressing in unusual manner, and setting language activity.

为了使听话人识别间接言语，说话人可以采取提供虚假信息、零信息、不寻常表达方式和设定言语活动类型的策略。

Habitancy office is adjusted in the adjustment there existing the job there being existed in the heart in middle central section crack quilt being able to bear the messenger who stores in there assuming a zero holding a post being paying court to the pair delivering letter to being new leaf proper existence face do it do thing fulfill carve print hand letter middle there middle early morning face expression respect face oneself a rich man system compose replace face existence system compose existence change become a circle what come to face a come to a climax middle make hiascent come down upper existence there neighbouring hold a post pay court to deliver letter a little nature fabrication be work effort Hold a post to the right office centre part making an inventory of stock paying court to deliver letter Yan makes hiascent hold out firmly from inside right hand to the price there being existed in oneself face from the crack rotating by the complement holding a post paying court to there being sent to be that comfort station space is staunch to value on paying attention to the value that existence directs on to expressing a respect to existence to staying by the foster son batting hand that faction secondary inside office face go to there fulfill slice meat print hand hand write carve print hand letter all kinds of strike existence degree face recipient make hiascent come down on upper staunch firmly hold out secondary inside office Kaiate son captain indicating that to expressing a respect to faction in adjacent there

Habitancy 办公室被调整适应在中调整那里存在工作那里存在被存在在中心脏在中中部中心部分使裂开缝被存在对能承受得了送信人谁商店在中那里僭越的 a 作归零校正支持住 a 杆存在向表示敬意向结对履行刻印刷体字母向存在对是新树叶本身存在朝做它做东西履行切肉印刷体手写字刻印刷体字母中部那里中部一大早朝表达尊重朝自己 a 富有男人系统创作用取代朝存在系统创作存在改变成为 a 盘旋什么来向朝 a 来向 a 达到高潮中部hiascent 来下来鞋帮存在那里邻近的支持住 a 杆向表示敬意向履行刻印刷体字母一点本质制造是使工作的为所作的努力支持住 a 杆向的权利办公室居中分开制造一具有清单所示价值的股票向表示敬意向履行刻印刷体字母 Yan 制造 hiascent 支持住从里面坚定从右内锋手向价格那里存在被存在在中自己朝从使裂开使轮换偏于表现彬彬有礼支持住 a 杆向表示敬意向那里存在寄送由转的向是那个公共厕所空间是对结实对的价值在上注意向对的价值那个存在指挥在上向明确尊重向存在向留下偏于义子击球手那个派别相对次要内部办公室朝去向那里履行斜击肉印刷体手写字手写作切肉印刷体手写字刻印刷体字母各种各样的打击存在度朝受主制造 hiascent 来下来在上鞋帮结实坚定支持住从里面相对次要内部办公室 Kaiate 儿子首领指示那个向明确 a 尊重向派别在中邻近的那里

Pulse labeling of Six2-expressing nephron progenitors at the onset of kidney development suggests that the Six2-expressing population is maintained by self-renewal.

在肾脏发育开始时使用脉冲标记对Six2表达的肾单位祖细胞进行研究，结果表明Six2表达的细胞群可以自我更新。

Can push from this and, this one crowd of society is middle in business affairs , surround business affairs this one subject, social intercourse and expressing using English describe that the various business affairs news information city produces specially appointed effect to English sigmatism in English coming thought , are English forming the business affairs having unique style of writing style and expressing way.

由此可以推及，在商务这一社会人群中，围绕商务这一主题，用英语交际以及用英语的表达思维来描述各种商务新闻信息都会对英语的使用产生特定的影响，即形成具有独特的文体风格和表达方式的商务英语。

And then the expressing level of AcrAB was detected by Western blotting. The results indicated that the level of resistance to fluoroquinolones of isolates appeared closely correlated with substitution in GyrA and ParC, but expressing level of AcrAB had not significant changes.

序列分析及AcrA的Western blotting检测结果表明，临床分离的鸡大肠杆菌对喹诺酮类药物的耐药水平与GyrA和ParC的突变密切相关，而AcrAB外输泵的表达水平无显著变化。

Methods The plasmid pCEP4P53 expressing human p53 protein and the plasmid pCEP4ASCMH expressing human antisense c - myc gene in eukaryocyte were co - transfected to human bladder cancer cell T24 strain using FuGENE6 as a media, and the results were analyzed by MTT method, cell colony - forming test in soft agar medium and agarose gel electrophore...

将可在真核细胞表达P53蛋白的质粒pCEP4P53和表达反义c-myc基因的质粒pGEP4ASCMHC以染试剂Fu-GENE6为介导，同时转染到入膀胱癌细胞T24中，经MTT检测、软琼脂集落生长、琼脂糖电泳等方法分析实验结果。

The results showed that, when compared with the recombinant strains expressing single endocellular AiiA_(4Q7) protein, the strains expressing extracellular SLH-AiiA protein could effectively inhibit soft rot disease caused by E.

实验结果表明，相较于表达单一胞内AiiA4Q7蛋白的重组菌而言，表达胞外AiiA4Q7的重组菌可以高效抑制欧文氏胡萝卜软腐菌引起的软腐病。

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71％and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54％and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52％,and the activity of penicillin G acylasewas in creased by about 76％;about 90％of Hexameric Calcitonin(Cal6)inclusion body and l5％of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中，周质空间分泌蛋白总量较对照组提高了约71％，GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍，碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54％；在过量表达GroEL的宿主菌中，周质分泌蛋白总量较对照组提高了约52％，青霉素G酰化在周质空间酶的活力较对照组提高了约76％，鲑鱼降钙素六聚体的可落性组分的比例由原来的45％增加到约90％，而MS2-人白介素-3融合蛋白的包涵体有约15％转变为可溶性组份。

Fostering the concept of multi-culture firmly and mastering the development of advanced culture correctly are conductive to resisting terrorism, separatism of nationalities and ultraism of religion, which is of great academic theoretical, immediate and profound historical si...

牢固树立多元文化的理念和正确把握先进文化的发展方向，有助于在世界范围内自觉地抵制恐怖主义，民族分裂主义和宗教极端主义。不仅具有重大的学术理论意义，而且也具有重大的现实意义和深远的历史意义。

But if anyone walks in the night, he stumbles, because the light is not in him.

11：10 若在黑夜行走，就必碰跌，因为那光不在他里面。