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By using Roche kit as standard, the amplification efficiency of different reagents were compared by Roche Light Cycler instrument for establishing real-time PCR reaction system. The primers were designed based on signature sequence of chromosomal DNA fragment and caf 1 gene in plasmid pMT1 of Y.pestis. And sensitivity and specificity assay were done with serial diluted Y. pestis EV76 strain and other bacterias. Then 275 Y.

采用SYBRGreenI随机掺入法，以Roche试剂盒为标准，比较两公司的DNA聚合酶，用鼠疫菌310 0 4建立荧光PCR反应体系；针对鼠疫耶尔森氏菌特异的染色体标识序列和质粒上F1抗原基因设计引物，检测其灵敏度和特异性，以盲测试验进行验证；在此基础上鉴定2 75株鼠疫耶尔森氏菌，并测试该法检测脏器污染模拟标本的灵敏度。

We construct a family of iterative mappings, which yield cyclic windows of variant size in the dynamical plane by incorporating cosine functions and non-linear angle variables with parameters. Chaotic attractors and filled-in Julia sets in the different cyclic windows are created by establishing the coordinates of any cyclic windows and the maximal window, then clarifying the relationship of corresponding points between these cyclic windows. The respective images in different windows are continuous but with individual structures.

该算法采用余弦函数及含参的非线性角度变量构造一族使动力平面上各周期窗口的尺度变化的迭代映射；通过计算任意周期窗口和最大周期窗口得出窗口间相应点之间的非线性对应关系，并构造出各周期窗口中的混沌吸引子和充满Julia集，其图案是连续的且结构不同的；选用不同的周期窗口作为基本计算区域，使之与正方形像素矩阵对应，并构造出基本图元，实现了用一个映射构造出多个平面排列图案的算法。

The limit point is analysed by establishing the mathematic model of cyclinder and taper orthoyonal.

通过建立圆柱与圆锥正交时的数学模式，对极限点进行几何分析，用微积分的方法求出极限点的坐标及变化规

Analysis of ThinPrep cytology in establishing the diagnosis of small cell carcinoma of lung.

TP液基细胞学在肺小细胞癌诊断中的作用。

Collect the Wei Xiaodong's lawyer of source attorney office to think, monitoring webpage is the responsibility of the website, issued 2000 according to MII " service of Internet electron announcement administers a regulation " medium concerned regulation, the person that electronic announcement service is offerred when the content that in discovering its electron announcement serves a system, appears to break the law apparently, should delete instantly, in last few years, network invite applications for a job is developing with swift and violent speed, the person that often have invite applications for a job is produced by bilk or dacoity case when applying for a job on the net, so, the buy before establishing examines link, undertake aptitude attestation to enterprise of invite applications for a job, market of normative network invite applications for a job, examine and verify of perfect network invite applications for a job and supervisory system extremely urgent.

汇源律师事务所的魏晓东律师认为，监控网页是网站的责任，按照信息产业部2000年颁发的《互联网电子公告服务治理规定》中的有关规定，电子公告服务提供者发现其电子公告服务系统中出现明显违法的内容时，当立即删除，近几年，网络招聘正在以迅猛的速度发展，经常有招聘者在网上找工作时被诈骗或抢劫的案例发生，所以，设立前置审查环节，对招聘企业进行资质认证，规范网络招聘市场，健全网络招聘审核和监督体系迫在眉睫。

He provided some use to establishing sketch customer interface can dalliance tool with use to is alternant with AutoCAD object to weave distance language.

他提供了一些用来创建图形用户界面的可拖拉工具和用来与AutoCAD对象交互的编程语言。

By introducing the concept of ideal transmission, the expressing way of meshing force and meshing damping is given, which is the foundation of establishing complex equation of gear dynamics.

通过引入理想传动的概念，给出了弧齿锥齿轮和准双曲面齿轮啮合力和阻尼力的表示方法，这一方法是复杂动力学方程建立的基础。

He was solely responsible for establishing the Delhi University Climbing Club and excelled at all the climbing and outdoor adventure course at the Himalayan Mountaineering Institute in Darjeeling.

他自发建立德里大学攀登俱乐部，在达吉岭的喜马拉雅登山研究所他在攀登和户外探险课程中表现优异。

By adopting the high performance of directional property and brightness of laser, a diagram for establishing a plumb datum line with the method of rotation and circle center tracking is presented.

利用激光本身的方向性好、亮度高等优点，提出以激光垂准仪为基本部件，用旋转跟踪圆心法建立铅垂基准线的方案。

According to the definition of the minimum zone method, the mathematical model is presented for evaluating flatness error and establishing datum plane equation.

分析了常用的近似评定法(三点法、对角线法、最小二乘法等)存在的局限性，根据最小区域法的定义，给出了基准平面方程及平面度误差评定目标函数数学模型的建立方法，并举例说明了采用Matlab进行平面度误差的计算。

He's a pretty westernised kind of guy.

他是一个非常西化的人。

National spirit is the moral kingpin for a nation to realize common ideal and goal.

一、中华民族精神的基本内涵党的十六大报告指出：在五千多年的发展中，中华民族形成了以爱国主义为核心的团结统一、爱好和平、勤劳勇敢、自强不息的伟大民族精神。