查询词典 envelope folder

The BDDcFⅧ gene was ligated behind PUB and 20H1 promotors to create lentiviral vectors pTK161 and pTK162. Meantime lentiviral vectors pTK161' and pTK162' were produced by cloning a green fluorescent protein into pTK151 and pTK152, which was driven by PUB and 2OH1 promotors respectively. Vector supernatant were prepared by using transfer calcium phosphate mediated-cotransfection of 293T cells. The virus vector,△NRF packaging-plasmid, and VSV-G envelope-plasmid was assayed by titers and cFⅧ activity in cell culture supernatant after infection into 293T cells.

用构建携带cFⅧ基因的慢病毒载体pTK161和pTK162（含20HI启动子），同时构建含绿色荧光蛋白的慢病毒载体pTK161'和pTK162'（含2OH1启动子）的方法，分别与包装质粒△NRF、包膜蛋白质粒VSV-G共转染293T包装细胞，将包装好的病毒颗粒再感染293T细胞，检测病毒滴度和培养细胞上清中cFⅧ活性。

With regard to the question how to cut the helical camber of screw-pump and spiral rod drilling tool in the field of petroleum machine and chemical machine, this paper firstly puts forward the cutting method how to apply the milling with non-instantaneous-pole envelope principle with plate milling cutter in our country.

本论文针对石油、化工机械领域螺杆泵、螺杆钻具转子的螺旋曲面切削加工问题，在国内首次提出了应用盘铣刀的无瞬心包络铣削加工方法。

Vaccinia virus belongs to the poxvirus family and has a double-stranded DNA genome of 192Kb in size. VV contains an envelope and replicates in cytoplasm of the infected cells. VV has a wide host range and infects various mammalian cell lines as well as animals.

牛痘病毒(Vaccinia virus， VV)属於痘病毒科中一拥有192kb双股DNA遗传物质的病毒，会在细胞质中繁殖出具有鞘膜的病毒，进而感染其他不同种的哺乳动物细胞和动物个体。

To save the pollen for later, brush it onto a piece of aluminum foil, fold the foil to make an envelope, and place in a freezer. It will last in the freezer for a month or so.

如果要保存这些花粉，那就把它们扫到铝箔上，折起来放冷藏吧，它们可以保存1个多月。

The RER, which is studded with polysomes, also seems to be the source of the nuclear envelope after a cell divides.

糙面型内质网，镶嵌在多聚核糖体中，也可能是细胞分裂后核膜的来源。

It costs nothing more than your time. No paper expense, no postage, no envelope expense.

它不需要你投资一分钱，需要投资的仅仅是你的时间，没有纸张、投递和信封的开销。

Be sure the envelope has enough postage for delivery to U.S.

请确认信封上有足够的邮费（这应该是指邮票吧，呵呵）以保证邮寄到美国。

It costs nothing more than your time. No paper expe e, no postage, no envelope expe e.

它不需要你投资一分钱，需要投资的仅仅是你的时间，没有纸张、投递和信封的开销。

If you would like an autograph or signed photo then please include a self addressed envelope with correct postage stamps attached.

如果你想要一张签名照的话请在来信中附着带有你自己地址邮票的信封

No paper expense, no postage, no envelope expense.

没有纸张、投递和信封的开销。

He has to relate with three women in the movie, and yet remain implicitly faithful to the unseen Lucinda.

让克劳德布里亚里成功地饰演试验了一个难度很大的角色：和三个女人打交道，但还要底下对观众未曾谋面的卢馨达保持忠诚。

The experiment was conducted to study effects of inulin on the growth performance, indices of immune organs and serum immunoglobulin of laying chicklings as well as the performance of inulin which replaced the antibiotics in diets by adding 0.2%, 0.4% and 0.8% of inulin, respectively.

试验旨在通过在蛋用雏鸡日粮中添加0.2%、0.4%和0.8%的菊粉，研究菊粉对蛋雏鸡生长性能、免疫器官指数和血清免疫球蛋白的影响，以及菊粉替代抗生素对蛋雏鸡的应用效果。