查询词典 effect binding

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

The feasibility of Ficks second law, the application of chloride binding isotherms and the effect of RAM have been discussed in this paper.

本文就Fick第二定律的适用性、氯离子结合理论的应用和海水耐蚀剂的作用等问题展开讨论。

EF116490. Characterization and comparison of this sequence with mouflons, goat, cattle and European bison orthologues were also conducted. Results showed that: 1 The 5'-flanking region contained many potential transcriptional factor binding sites such as those for C/EBPb, C/EBP, SP1, Cap, USF, HFH-2, HNF-3b, and Oct-1, which might have an important effect on transcription activation and regulation as well as tissue-specific expression.

EF116490，在分析其序列结构特征的基础上与GenBank中摩弗伦羊、山羊、普通牛、欧洲野牛进行了比较基因组学和系统进化研究，结果表明：(1)欧拉型藏绵羊GHR基因启动子P1区存在C/EBP、C/EBPb、SP1、Cap、USF、HFH-2、HNF-3b、Oct-1等多个潜在的转录因子结合位点，可能与GHR基因的转录调控和起始以及特异表达有关。

The morphology of virions seems to not be change after incubated with the paeonol by electron microscope. The regained infectivity of TMV recovered by dialysis from incubation with paeonol in vitro showed that the paeonol have not direct viricidal effect. The paeonol did not reduce infectivity when applied before TMV inoculation. The paeonol in low concentration can restrain the polymerization of TMV coat protein in vitro. The paeonol did not reduced infectivity of TMV-RNA in vitro. It is proposed that the paeonol inhibited TMV by binding with TMV coat protein to interfere with recognition of a host receptor essential for infection or by interfering TMV-CP polymerization in plants. The anti-TMV activity of low molecular weight chemicals extracted from Polygonum cuspidatum were determined.

丹皮酚与TMV混合，电镜下观察对粒体形态没有影响；TMV与丹皮酚混和后透析可恢复侵染力，表明丹皮酚对病毒粒体并没有直接毒害作用；预先用丹皮酚喷施叶面不影响病毒的侵染，表明丹皮酚未干扰TMV对叶片侵染位点的识别；丹皮酚在低浓度时可阻碍TMV-CP的体外聚合，对TMV-RNA并无体外抑制侵染作用，据此推测丹皮酚对TMV的抑制作用可能主要通过其与TMV-CP结合影响病毒粒体对叶片表面侵染位点的识别，或影响体内TMV粒体的脱衣壳或TMV-CP亚基的体内聚合。

He results show that the virus regained infectivity, and the tannins did not direct viricidal effect. Tannins extracted from Polygonum perfoliatum or Archontophoenix alexandrae seeds showed strongly anti-TMV activities separately. TMV virions seems to be aggregated when TMV incubated with the tannins by electron microscope. It seems that TMV coat protein bind to the tannins based on PAGE. Tannins did not reduce infectivity when applied either before or after TMV inoculation. It is proposed that tannins extracted from the 11 species inhibited TMV by binding with TMV coat protein to interfere with recognition site essential for viral coat protein. Tannins extracted from Euphorbia hirta, P. perfoliatum, P. cuspidatum reduce infectivity when applied immediately in N.

MV分别与这11种植物单宁混合后，通过透析除去单宁后，可恢复大部分侵染力，表明单宁对病毒粒体并无直接毒害作用；TMV与杠板归和假槟榔种子中提取的单宁分别混合后，电镜下观察粒体呈现明显的聚集现象；TMV外壳蛋白分别与这两种植物单宁混合后，聚丙烯酰胺凝胶电泳中CP泳带滞后；单宁在病毒接种前施用未降低TMV对叶片的侵染能力；这些结果表明植物单宁对TMV抑制作用可能是单宁与TMV-CP结合影响病毒对叶片侵染位点的识别而降低TMV侵染性，而这种结合是可逆的。

The stablest complex has been predicted by using the formation energy and the binding energy. The mechanism of the hydrogen passivation effect was also briefly discussed in our present study.

通过形成能和束缚能的计算，找到了复合杂质稳定存在的形态，分析了H对阳离子空位缺陷的钝化作用的过程和起因。

The research of chloride binding isotherm and the effect of RAM in this paper is valuable for the improvement of Ficks second law, and new contents have been established for the definition of pouring.

本文氯离子结合理论、耐蚀剂作用等研究为改善Fick扩散模型提供了理论支持，同时提出淋溅作用的概念，为氯腐蚀环境下混凝土结构耐久性研究提供了新的内容和发展空间。

The results show that, the Fick's second law based on corrosion environment should be presented; the linear isotherm of chloride binding is appropriate in concrete structures; the amounts of bound chloride in tide range area and air environment is 0.11~0.37 times of free chloride; the diffusion coefficient is 1/3 to the concrete without RAM and the effect is the best when the RAM ratio is about 15%.

研究表明：Fick第二定律仍需进一步改善，有必要根据不同侵蚀环境建立不同的侵蚀模型；用线性结合理论描述实际混凝土结构中的氯离子结合基本适用；潮差区及以空气中氯离子侵蚀为主的混凝土结构，结合氯离子占自由氯离子的相对值大致在0.11～0.37之间；掺加海水耐蚀剂混凝土的氯离子扩散系数大致可降为原来的1/3；海水耐蚀剂含量在15%左右时其提高混凝土抗氯离子渗透性能的效果最明显。

The results show that, the Ficks second law based on corrosion environment should be presented; the linear isotherm of chloride binding is appropriate in concrete structures; the amounts of bound chloride in tide range area and air environment is 0.11~0.37 times of free chloride; the diffusion coefficient is 1/3 to the concrete without RAM and the effect is the best when the RAM ratio is about 15%.

研究表明：Fick第二定律仍需进一步改善，有必要根据不同侵蚀环境建立不同的侵蚀模型；用线性结合理论描述实际混凝土结构中的氯离子结合基本适用；潮差区及以空气中氯离子侵蚀为主的混凝土结构，结合氯离子占自由氯离子的相对值大致在0.11～0.37之间；掺加海水耐蚀剂混凝土的氯离子扩散系数大致可降为原来的1/3；海水耐蚀剂含量在15%左右时其提高混凝土抗氯离子渗透性能的效果最明显。

From May 4th Movement of 1919 to the 1920s, sex love narrations in Chinese novels were rather an enlightenment and liberation in that particular era with four characteristics. Firstly, sexual desire was endowed with rationality with regard to liberation of human nature and mutual love. Secondly, with an epochal concern for a stronger and superior race, greater attention was paid to sexual parties concerned, mainly physically. Thirdly, new ethics on sex were promoted in that personal freedom on sex love was emphasized and sex love became an important way of self-fulfillment. Therefore, independence of sex love was advocated and the scope of sex love was also widened. Fourthly, the sex consciousness of this period had the mixed features of the past and the contemporary. Sex love narrations in the 1930s and 1940s showed a further exploration on the theme of human nature. First of all, sex was restored to a kind of desire. ShenCongwen probed the importance of harmonious sex love to a sound personality and Shi Zhecunrevealed that sexual inhibition could lead to the distortion, even frenzy of human nature. Bothexplored the influence and functions of sexual desire as human nature upon human personality,giving prominence to the irresistibleness of sexual desire and the significance of sexual desire asa motive. Then, human bodies driven by sexual desire were described and given greater attentionto. Next, ethics on sex were deconstructed either by Shen Congwen who opposed man-madeethics with law of nature and the School of New Sensation who replaced traditional ethics on sexwith exchange rules in consumption, or by Shi Zhecun who spared no efforts in representing thecomplete failure of social rules, including ethics on sex, due to the impact from sexual desire.The direct effect was a paleness of the ethical binding force on sex. Last, sex consciousness inthe works of female writers emerged. In the early works of Ding Ling, the sexual desire of thefemale is no longer a ramification of emotion but an independent one. In addition, in the sex lovenarrations of other female writers, such as Mei Niang, strong self-consciousness on the socialstatus of the female is also reflected. Sex love narrations in the late 1970s and early 1980s wereactually a re-enlightenment in the spirit of humanism, whose major features include humanisticlegitimation of sex love within the boundary of ethics, a blend of sex love narrations withnational concepts and national myths as well as the aesthetic, poetic descriptions of humanbodies etc. In the middle and late 1980s, sex love narrations entered a stage of exploration forpluralism. First, by restoration of sexual desire, writers of this era restored human nature, thevery original state of human being. Different emphasis and tendencies could be seen in differentwriters: looking at sex love dialectically and trying to describe its creative as well as destructivecharacteristics; connecting sex love with violence and trying to reveal the evil side of humannature through it; emphasizing the creativeness of sex love especially in the heritage of"wildness".

从&五四&到20世纪20年代，中国小说的性爱叙事是一种处在时代夹缝中的启蒙和解放，其所表现出来的特点，一是正面赋予性欲望以合理性，这种合理性是在人性解放和爱情前提下获得的；二是在强种育种的时代焦虑影响下小说性爱叙事表现出对物质身体的关注，其焦点主要在体格方面；三是树立起新的性伦理道德观念，表现为强调在性爱问题上的个人选择自由，性爱成为个人自我实现的重要方式，从而赋予人在性爱问题上的独立性，同时还扩大了性爱表现的领域；四，这个时期小说性爱叙事中所表现出的性意识还具有新旧杂糅的特点。30、40年代小说性爱叙事表现出对人性主题的深化与丌拓，首先是把性还原为欲望本身来探索，沈从文从和谐性爱对健全人性的决定作用、施蛰存从性压抑会导致人性扭曲甚至疯狂的角度探讨作为人类本质性欲望的性对人性产生的影响和作用，突出了性欲力量的不可抗拒和作为人类行为的重要动力等方面的内容；其次在叙事中表现出对欲望化了的身体的描绘与关注；第三是解构了性的伦理道德，无论是沈从文以自然法则来反对人为的道德设置还是新感觉派用消费交换原则替代传统性伦理道德，或是施蛰存极力表现包括性伦理规范在内的社会法则在性欲力量冲击下溃不成军，其直接的效果都是使性伦理道德对人的约束力量显得苍白虚弱；第四，出现了由女性作家表现的女性性意识，在丁玲的早期作品中女性的性欲望不再是作为感情的衍生物而是独立的欲望存在，而在梅娘等其他女作家的小说性爱叙事中则表现出对女性社会地位的强烈自觉意识。70年代末80年代初期小说的性爱叙事实际上是在人道主义语境下的再次启蒙，其主要特征一是性爱在人道主义前提下获得合法性，但要求性爱不超出伦理规定的范围；再就是将性爱叙事同国家观念和民族神话相融合；此外还有对身体的描写审美化诗意化等。80年代中后期，小说性爱叙事进入了多元化探索的阶段，作家通过还原性欲望来还原人性、还原人类的本真状态，不同作家笔下表现为不同的侧重和倾向——以辨证的方式看待性爱，写出其具有的创造性力量和毁灭性特征；将性爱与暴力相联系并通过其来表现人性的黑暗；强调性爱的创造性作用，尤其在民族&血性&的传承方面。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。