查询词典 effect binding

Seven colorimetric methods Biuret, Lowry, a modified Lowry technique using bicinchoninic acid, Coomassie brilliant blue G (CBBG dye-binding in phosphoric acid, perchloric acid or hydrochloric acid, and bromophenol blue dye-binding were evaluated for determination of protein concentration in human whole saliva.

本实验以七种定量蛋白质之方法（Biuret法，Lowry法，BCA法，CBBG染料结合法、BPB染料结合法），利用三种参考蛋白质评估测定人类唾液蛋白质浓度之差异性。

All experimental results show that the mode of binding of Sm3 to DNA is mainly electrostatical binding.

实验综合表明，Sm3与DNA之间的结合主要为外部静电作用方式。

Objective To express and locate a fusion gene consisting of c-Myb DNA-binding domain and Myeloid elflike factor AML1-binding region by using baculovirus vector.

目的 将C-Myb的DNA结合域与MEF的AML1结合区组合成的MM融合基因在杆状病毒中进行表达和细胞定位。

Results: Mel specific binding sites were det ermined in 16 kinds of human embryonic tissue and this binding could be inhibit ed by GTPγS, supporting the theory that MR is coupled to inhibitory G-proteins system.

结果：经饱和分析、动力学分析及特异性分析证明人胚胎心、肝、肾及甲状腺等16种组织均存在Mel特异结合位点，亚细胞分布研究表明这些组织中以细胞核及线粒体MR含量最高；GTPγS能够抑制Mel与其受体的结合，使MR的结合容量或亲和力降低。

Esculetin had the highest affinity toward the binding site of XO, and this was due to hydrogen bonding interactions. The hydroxyl group at C-6 formed hydrogen bonds with the carboxyl group Glu802 that played critical role on XO binding.

在8种香豆素衍生物中发现esculetin与黄嘌呤氧化酶的亲和力最高，因为在esculetin的结构上，C-6的OH基团可与麸胺酸802的COOH基团形成氢键，此键结对於抑制黄嘌伶氧化酶活性是很重要的，因而增加了两者结合的稳定性。

As well using flameless atomic absorption spectophotometer toanalyse the amount of platinum binding to DNA,we can fand that after 1hour at 37℃ the amount of platinum binding to DNA for BGC-823 acted byCDDP 20ug/ml in hypotonic solution was 30.1±6.2ng/mg·DNA,whilethe isotonic groups were 6.4±1.5ng/mg·DNA.

采用非火焰原子吸收光谱法检测DNA结合铂含量发现，BGC-823经20μg／ml CDDP，37℃作用1小时，低渗给药组DNA结合铂含量为30.1±6.2ng／mg·DNA，等渗给药组DNA结合铂含量为6.4±1.5ng／mg·DNA，两者差异显著(P＜0.05)，该结果表明低渗溶液能显著增强CDDP与其作用靶点—DNA的交联。

However, the binding patterns between the HU-induced and uninduced HEL cells were different. The data in Southwestern-blot analysis showed that the molecular weights of the GATA factors binding to the HS2core DNA sequence were 43kd and 47kd respectively.

反义寡核苷酸技术及RT-PCR的结果表明，GATA-1的反义核苷酸抑制了羟基脲诱导的β-珠蛋白基因的表达，进一步证明了GATA-1因子在羟基脲诱导的HEL细胞分化过程中是不可缺少的促进因素。

Biotinylated lectins:UEA-Ⅰ, RCA-Ⅰ, DBA, PSA, PNA, BSL, LCA, WGA, ConA AND SBA were used as probes to identify lectin binding sites and to determine if lectin binding patterns change with age in the developing of human fetal esopha geal epithelium (8W, 14W, 20W, 28W and 32W).

采用十种生物素化凝集素UEA-I、DBA、PSA、B SL、PNA、LCA、RCA-I、SBA、ConA及WGA分别对8W、14W、20W 28W及32W的人胎儿食管上皮进行研究，以确定胎儿食管上皮在发育过程中凝集素的结合位点以及结合方式随年龄的变化。

METHODS: Cytometry and soft agar were employed in observing the biological characters of MEC-1 and Mc3 cell strain. The expressions of the two sub-types of MEC-1 and Mc3 cell strain, SSTR1 and SSTR2, were detected by hybridization in situ. The condition of the binding between MEC-1, Mc3 and 125I-RC-160 was analyzed by radioactive genin binding analysis.

采用细胞计数法、软琼脂法等观察MEC-1及Mc3细胞株生物学特性；以原位杂交法检测MEC-1，Mc3细胞株SSTR1及SSTR2两种亚型的表达情况；以放射配基结合分析法分析MEC-1，Mc3细胞与125I-RC-60的结合情况。

The results showed that though apoHb could be reconstituted with all FePs, it showed different functions. Binding of apoHb with hemin can enhance the heterolytic cleavage of O-O bond of H〓O〓, but inhibiting the cleavage when FeTCPP and FeTPPS〓 were used instead of hemin. The binding of apoHb with FeTTMAPP showed negligible influence on the cleavage of O-O bond in H〓O〓.

结果表明，虽然具有阴离子基团的FeTCPP和FeTPPS〓能够在天然血红蛋白中辅基位置的附近与脱辅基血红蛋白结合，但是由于结构上与天然辅基的差别，使得血红素蛋白中相应的氨基酸残基不能起到有效的作用，因此不但不能提高反而抑制了其过氧化物酶活性；而对于具有阳离子基团的FeTTMAPP，脱辅基血红蛋白对其活性没有明显的影响。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。