查询词典 effect a permanent cure

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

This text discussed how to choice the material of permanent magnet and transmit-magnet, design the circuitry of magnetism and the method of demagnetization and so on. There emphasize introduce the structure of permanent magnetism cupule of crane that using AlNiCo permanent magnet or NdFeB permanent magnet, application of RLC circuitry in using pulse to demagnetization and how to realize the lighter to weight, lower to cost and prolong using life for permanent magnetism cupule of crane by way of choosing in reason the parameter of R, L, C etc.

本文就节能、省材、安全的起重永磁吸盘设计制造中有关永磁材料与导磁材料选用、磁路设计组合、永磁体的充退磁方法等问题进行了讨论，着重介绍了用电脉冲充退磁的铝镍钴永磁、钕铁硼永磁或二者组合式的起重永磁吸盘的结构设计、RLC电路在脉冲充退磁技术中的应用，如何合理选用R、L、C等参数以实现起重永磁吸盘的小自重、低成本及长寿命。

I have engaged in the research of rice stripe blight control for many years in Shanghai farm and found some problems: though there were some reports of research on rice stripe blight resistance, strategy of prevention and cure for the disease has been face to farmer mainly, while geographical location, cultivars and cultivation system adopted on the Farm all affect the effect of control. On the other hand, our Farm has paid attention to the chemical prevention and cure for rice stripe blight and ignored other measures. As for the chemical prevention and cure, phenomenon of drug-resistance and bad effect of prevention and cure existed usually.

本人在上海农场多年从事该课题研究过程中发现的问题：目前对水稻条纹叶枯病研究的报道较多，在防治对策上主要是针对农户，缺乏关于农场地域因素、品种、栽培制度等因素对水稻条纹叶枯病发生及防治的影响的报道；在水稻条纹叶枯病的防治上，农场仍是以化学防治为主，对其他防治措施还不够重视；在化学防治过程中，存在抗药性、防效不佳等问题。

Firstly, by using DSC, we analyzed the effect of the type and content of initiators on the curing reaction, Then, through FTIR, we discussed the final degree of cure of the cured resin and the performance of the cured resin by testing the static mechanical performance and DMTA, found that moderate content and ratio of the initiators system can cause the moderate cure temperature, increase of exotherm and the final degree of cure result from the cooperation effect of the two kinds of initiators, and found that the cooperated initiators system can effectively increase the final degree of cure even in low content.

通过差示扫描量热法分析了不同固化剂体系对树脂固化行为的影响，用红外光谱分析了不同固化剂体系下的浇铸体的固化度，并对树脂浇注体静态力学性能及动态机械热分析，认为不同活性的固化剂联用存在协同效应，使固化反应温度适中，固化反应更加充分，提高树脂固化度和扩大聚合物网络，使树脂浇注体拥有良好的力学性能和耐热性。

People have paid attention to the pollution of soil and water quality caused by using organic arsenic chemistry reagent to prevent and cure apple-tree canker in our apple area, so it has an important purpose to seek a harm-free way for sustaining development of apple. The tests studied the effect of prevention and cure on apple-tree canker with EM mud, EM liquid, 40%'Fumeishen powder' and contrast. The results showed that the EM mud was superior to the EM liquid, 40% 'Fumeishen powder' and contrast. The prevention and cure effects of EM in 2006 and 2007 were 92.7% and 93.5%, while the recurrence rates of canker scars 1.8% and 2.8% respectively. And the healing effect of scar is 50.4%. So, it was considered as a new way to prevent and cure apple-tree canker with EM mud.

中国各苹果主产区果农长期使用有机砷等化学制剂防治腐烂病，对土壤、水质等环境造成的污染已引起人们的关注，寻求无公害的方法防治腐烂病，对苹果业可持续发展具有重要意义；本试验设EM活性菌泥涂抹、EM活性菌液涂抹、40%福美砷可湿性粉剂50倍液刮涂和对照（只刮除腐烂病疤，不涂药）4个处理，三次重复，进行了苹果树腐烂病的防治研究；结果表明，EM活性菌泥在防治效果、病疤复发率和促进病疤愈合效果方面均优于其它处理，其当年和第二年4月的防治效果分别为92.7%、93.5%，病疤复发率分别为1.8%、2.8%，促进病疤愈合效果也最好（50.4%）；因此，作者提出，将EM活性菌融入粘泥载体中制成菌泥，涂抹在腐烂病疤上，以菌治菌，是防治苹果树腐烂病的新途径。

Cent of patient of 51 breast cancer is the method two groups, use medical group of biology albumen rubber 25 routine breast cancer is improved effect a radical cure glue of use biology albumen is achieved in the operation when art, did not use medical group of biology albumen rubber 26 routine breast cancer is improved effect a radical cure biology albumen gum is not used when art, the 1st day drainage after observing art always makes the discharge, hypodermic case that accumulate fluid after quantity, art, in order to decide medical biology albumen glue is improved to breast cancer the precautionary effect that effects a radical cure fluid is accumulated hypodermically after art.

方法51例乳腺癌患者分为两组，采用医用生物蛋白胶组25例行乳腺癌改良根治术时在手术创面使用生物蛋白胶，未用医用生物蛋白胶组26例行乳腺癌改良根治术时不使用生物蛋白胶，观察术后第1天引流量、术后总引流量、皮下积液情况，以确定医用生物蛋白胶对乳腺癌改良根治术后皮下积液的预防效果。

As a result the time great majority of occurrence chyle bosom is 10d of the 2 ～ after art, 26 classics guard cure to cure, 5 open a chest again, among them 4 are cured, after an art still reappearance, continue to guard cure to cure.

结果出现乳糜胸的时间大多数为术后2～10d，26例经保守治疗治愈，5例再次开胸，其中4例治愈，1例术后仍再发，继续保守治疗治愈。

On the analysis foundation for the at the same time generator control law of permanent magnetisms have discussed the at the same time generator word of permanent magnetism of niter quantity to melt the function of every control son modular in control system and the specific ay of realizing, have put forward a set of at the same time generator word of permanent magnetism of entire quantity to melt the specific enforcement of control scheme, and according to this set of scheme having established the synchronous generator of permanent magnetism based on Simulink the word of entire quantity melts to control emulation model, for the control made scheme emulate to analyses.

在对永磁同步电机控制规律的分析基础之上，讨论了永磁同步电机全数字化控制系统中各个控制子模块的功能和具体的实现方式，提出了一套永磁同步电机全数字化控制的具体实施方案，并根据这套方案建立了基于 Simulink的永磁同步电机全数字化控制仿真模型，对所出的控制方案进行了仿真分析。

Came in April 2006 in September 2008, the malign chest that we use RIL-2 to arrange cure of the inject inside platinic thorax to cannot be able to bear or endure to suffer the whole body change cure or do not wish to accept the whole body to change cure jointly accumulates fluid patient 26, curative effect satisfaction.

梗概： 2006年4月至2008年9月，我们采用rIL-2联合顺铂胸腔内注射治疗不能耐受全身化疗或不愿接受全身化疗的恶性胸腔积液患者26例，疗效满足。

JMA not only brings into play importance rules of qi-benefiting and surface-defending to effect a permanent cure on protection function of eye that gives attention to heat-clearing and toxin-removing to remove evils for temporary solution, it also treats both principal and secondary aspects of the disease, which really embodies a basic academic viewpoint of traditional Chinese medicine that"Fu Zheng Qu Xie" under the wholistic concept of Chinese medicine can serve to prevent and cure HSK recurrence.

角膜安不仅发挥了组方中益气固表以扶本护目之整体功能的作用，而且兼顾了清热解毒以祛邪治标的作用，祛邪与扶正同施，标本兼治，充分体现了中医药整体观指导下的&扶正祛邪&防治HSK复发的基本学术观点。

His female bullfighter Lydia gradually Health love, but she was in a vegetative state after the Bulls Zhuangcheng away from her, just because Lydia's ex-boyfriend showed more affectionate than he is like love.

他对女斗牛士莉迪亚渐生爱意，却在她被公牛撞成植物人后离她而去，仅仅因为莉迪亚的前男友表现出好似比他更深情的爱。

I know of none which are not easy to answer, but what I am trying to do now is only by a series of hints to throw some light on the narrow path of legal doctrine, and upon two pitfalls which, as it seems to me, lie perilously near to it.

我不认为有什么难以回答的问题，但我现在要做的仅仅是通过一系列线索，来给法律理论的狭窄路径以及在我看来离它近得危险的两个陷阱提供一些启示。