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The optimum condition of the ELISA are set as follows: 1ug/mL of NFLX -HSA(norfloxacin -human serum albumin) conjugation used as a coated antigen dissolved in carbonate buffer solution(CBS,pH 9.6,0.05mol/L), 0.5 % glutin in PBS(pH7.4,0.01mol/L) as a blocking agent post-coating, horse radish heroxidase-labeled sheep anti-mouse IgG(HRP-anti IgG) diluted by PBS(pH 7.4,0.1mol/L) in 1:1000 as a secondary antibody, and TMB (3,3",5,5"-tetramethyl benzidine dihydrochloride) as a sbustrate.

成功建立了检测抗诺氟沙星抗体的间接ELISA方法，其主要条件如下：pH9.6、0.005mol／L的碳酸盐缓冲溶液为包被液，1μg／mL的连接抗原NFLX-BSA(诺氟沙星-牛血清白蛋白)为包被抗原，1％明胶为封闭液，1：1000稀释的HRP酶标记的羊抗小鼠抗体作为二抗，TMB(3，3，5，5-四甲基联苯胺)作为底物。

Almost all of the reforms he proposed during the first three years of his presidency were diluted in the legislature, and his conservative National Action Party has just lost a midterm election.

他在前三年总统任期中所提出的改革几乎无一例外的最后都在立法上夭折，而他的保守派国家行动党最近又在中期选举中铩羽而归。

We painted the sticky liquid on their bodies, and covered them with the toilet paper which would be diluted by the water in those lovely balloons after they broke up.

六点开始给演员化妆，继续用黏液涂抹在她们身上，并用卫生纸简单覆盖沾粘，它们会被预埋好的可爱的注水球稀释。

Thereafter, aliquots of diluted blood sampleswere incubated with 3 different concentrations of fibrinogen(0.75, 1.5, and 3.0 mg/mL).

此后，三份稀释血样用3个不同浓度的纤维蛋白原（0.75，1.5，3 mg/mL ）培养。

Aliquots of diluted bloodsamples were incubated with two different doses of fibrinogenconcentrate, FXIII concentrate, the combination of both, orwith two different doses of FFP.

稀释的等量血液样本分别加入两种不同剂量的纤维蛋白原浓聚物， F ⅩⅢ浓聚物，或联合应用，或两种不同剂量的新鲜冷冻血浆。

Result A strain of Pseudomonas cepacia was detected in 1.6% compound quaternary ammonium disinfectant before application. The amount of the germ was 1.21×105cfu/ml. The strain had the similar resistance to chlorine-containing disinfectants and glutaraldehyde compared with Staphy aureus. The contaminated disinfectant could still thoroughly destroy the antigenicity of HBsAg with 60min's contact after diluted for 5-20 times.

结果 该洋葱假单胞菌对氯和戊二醛的抗力与大肠杆菌和金黄色葡萄球菌接近，污染样液除菌后1 000倍稀释对大肠杆菌和金黄色葡萄球菌仍有较好的杀菌效果，20倍稀释可完全破坏HBsAg抗原性，和抑制枯草杆菌黑色变种芽胞的生长，1 600倍稀释可抑制白色念珠菌生长，而原液则不能抑制分离菌株的生长。

It was dissolved in propylene glycol and diluted with water to form a disinfectant solution.

氯羟二苯醚为白色结晶粉末，以丙二醇溶解，再用水稀释制成消毒液。

One of them is based on etched porous alumina and carbon-gold-carbon compound film. The alumnia film surface is composed of rows of valleys with diameter of about 300 nm, and is realized by etching porous alumina in diluted phosphorous acid.

第一种阴极采用基于多孔氧化铝和无定形碳的双层传导薄膜，其中氧化铝薄膜的表面具有横向尺寸为300 nm的特殊凹陷结构，是对常规多孔氧化铝薄膜进行腐蚀形成的。

The sample was diluted by water containing a little amount of sodium chloride, and then extracted by hexane, purified by oil of vitriol to decrease impurity. The extracted solution was analyzed by GC-ECD and quantified by external standard method.

试样加水稀释，加入少量氯化钠，再用正己烷提取，浓硫酸净化样品，排除干扰杂质，以GC-ECD测定，外标法定量，六六六和滴滴涕在0.005~0.2μgmL^(-1)范围内，质量浓度与峰面积呈良好的线性关系。

The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study. The different tissues of grasses were immersed in 0.1% of SDS for 15 min, 3% of NaClO for 3 min, 0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10^(-3) and using TSA culture medium for 5 to 7 days under 28℃.

通过对高寒牧草内生细菌的分离培养方法研究，得出了优化的分离培养条件，即牧草不同组织器官用0.1%SDS浸泡15min、3%NaClO浸泡3min0.1%升汞浸泡10min、75%酒精1~2min处理后（各步间均用无菌水冲洗3~4次），研磨并稀释至10^(-3)，涂布于TSA培养基上，置28℃恒温条件下培养5-7d，可从高寒牧草组织中分离获得数量、种类较多的内生细菌。

Connectors are lines that can be anchored to particular places, called glue points , on the graphic object.

连接符，可固定在图形对象的特定的地方的线条。

Another way of putting it, too many agents have a say in each other's work, and bureaucratic rigor mortis sets in.

另一种表达是，太多的作用物在彼此的工作里都有话语权，官僚的僵尸开始抬头。