查询词典 digested tankage

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

All tankage and connecting flumes are boiler plate.

所有筒槽和与之相连接的水槽为平轧钢板制造。

However, in these cases, it may be more economical to use one or more larger diameter Bio-Module shafts mounted in concrete tankage Figure No.

但是，这种情况下，将一个或更大直径的生物模块轴安装在水泥池中会更节省成本。

A Bio-Clarifier shaft is supplied to be installed in concrete tankage.

生物二沉池的轴安装在水泥池中。

Maximum capacity of flow equalization limited by available wastewater depth in feed chamber of concrete tankage.

水泥池的进水箱内的水深决定水流均衡区的最大承受能力。

Fluids contained within the ballast tanks are utilized to provide buoyant support for process tankage.

利用在压载箱内所装液体来对处理的容器设备提供浮力支承。

All necessary non-processing facilities: adequate tankage for storing crude oil, intermediate, and finished products; a dependable source of electrical power, material-handling equipment

所有必须的非工艺设施；足够的储罐容量用以储存原油、中间产品和成品；可靠的电力和物质处理设备来源

Pretreatment for the RBC package plant is required to remove large, dense solids which might otherwise settle out in the RBC tankage or become lodged in the media.

要求RBC打包工厂的预处理可以去除厚大的固体，否则这些固体会沉降在RBC池底部或积聚在盘片中。

In addition, a complete refining installation must include the following: all necessary non-processing facilities; adequate tankage for storing crude oil, intermediate, and finished products; a dependable source of electrical power, material-handling equipment; workshops and supplies for maintaining a continuous 24 h/day, 7 day/week operation; waste disposal and water-treating equipment; and product-blending facilities.

此外，一套完整的炼油装置须包括以下设备：所有必须的非工艺设施；足够的储罐容量用以储存原油、中间产品和成品；可靠的电力和物质处理设备来源；用于维持一天24小时，一周七天连续运转所需的车间和物资；废料和污水处理设备；以及产品混合设施。

That snake Alaric named Archbishop...

庇护。。。那个毒如蛇蝎的Alaric被命名为大教主。。。

You have done lots of magical things to be amazed at.

你做了很多神奇的事物感到惊讶。