查询词典 detected

Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠，融合筛选抗DR5杂交瘤细胞，制备抗DR5单抗——mDRA6；流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率；荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化；MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响；琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Methods One hundred and sixteen unclassified arthralgia/arthritis patients were studied and followed up for 4 to 8 months.APF and AKA were detected by indirect immunofluorescence,anti Sa and anti RA33/36 antibodies were detected by immunoblotting.

到目前为止还没有一个可作为常规的比较特异的诊断手段，特别是在疾病的早期阶段仍比较困难，而早期有效积极的治疗能很大程度上改善RA的预后，至少能延缓病情的进展［1］。

Many samples that are not detected by known AVs are detected by Twister.

许多知名AV没有检测到的样本费尔都检测到了

Methods:180 HCV patients and 40 healthy controls were selected for the research, and their serum BAFF levels were detected by ELISA. The level of serum HCV-RNA in HCV patients was detected through fluorescent quantitative polymerase chain reaction and analyzed along with the clinical data of HCV patients.

选取180名HCV感染者及40名健康对照，应用酶联免疫吸附法测定血清中BAFF含量，荧光定量聚合酶链反应检测HCV感染者血清中HCV-RNA的水平，并结合HCV感染者临床情况分析。

Methods The immobilizing conditions of neutrophil alkaline phosphatase staining were detected by the stationary liquid of 40%, 60% and 80% acetone-citric acid, and at the same time, the staining conditions were detected at 10, 15 and 20 minutes in the matrix liquid prepared respectively with 2-amino-2-methyl-1.3-propylene glycol (PH9.4-9.6) buffer, barbital (PH9.2) buffer and Tris (PH9.2) buffer.

用40%、60%、80%的丙酮-枸缘酸固定液对中性粒细胞碱性磷酸酶染色固定条件进行测定。同时用2-氨基-2-甲基-1.3-丙二醇(pH9.4-9.6)缓冲液、巴比妥(pH9.2)缓冲液、Tris(pH9.2)缓冲液配制的基质液分别在10、15、20分钟的染色条件进行测定。

Total Protein was detected by biuret and Albumin was detected by bromocresol green.

静脉血样本来自30头贵州小型猪和46个健康人。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物，利用PCR扩增方法，以beta-actin做内参，检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况，发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致，表明各模板DNA量基本一致；而在同一DNA浓度下，16S rDNA的扩增片段长度虽一致，但其产物量存在明显差异，精子16s rDNA产物量显著低于其他3种组织，其条带宽度和亮度很弱；16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

The carboxyhemoglobin levels in artery blood were detected with CO-oximeter and the expression of intercellular adhesion molecule-1 (ICAM-1) in the lung was detected by Western blotting.

应用一氧化碳血氧分析仪监测动脉血中碳氧血红蛋白水平的变化；应用Western blotting检测肺组织中细胞间粘附分子-1（ICAM-1）表达的变化。

50 Eyes (32 cases) with glaucoma and 32 eyes (20 cases) with suspected glaucoma were detected by TBC-1 A automated computerized centrical perimeter. The results showed that:(1) testing visual field defect ratio were 100% in glaucoma.(2) earliest glaucoma field changes were not detected in suspected glaucoma.

应用国产自动微机视野仪TBC-1A型中心视野仪，检测青光眼32例50眼，视野缺损检出率为100％，可疑青光眼20例32眼，没有检出极早期青光眼视野变化，但经6～18个月追踪观察，其中5例8眼出现青光眼视野缺损。

The second is to cut a mere 100 calories from your diet , which is the equivalent of a tablespoon of butter.

第二个就是从你的饮食中减少一百卡路里，只是相当于一大汤匙的黄油。

This is where the Rorschach test comes in.

现在，罗氏测试就出现了。