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From the second week on, the Apomorphine induced rotation behavior is detected once a week in the second, third, and forth week after the operation.

从术后第2周开始,分别于术后第二、三、四每周一次检测动物的Apomorphine诱导的旋转行为。

From the second week on, the Apomorphine induced rotation behavior is detected once a week in the second, third, and forth week aft...

从术后第2周开始,分别于术后第二、三、四每周一次检测动物的Apomorphine诱导的旋转行为。

Methods:All of 40 male Sprague-Dawley rats were divided into 3 groups:control group(group A, n = 10), diabetic group for 4 weeks(group B, n = 14), and diabetic group for 12 weeks(group C, n = 16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NF-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4周组(14只),C组为糖尿病12周组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后,A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB 活性检测。

Methods All of 40 male Sprague-Dawley rats were divided into 3 groups: control group (group A, n=10), diabetic group for 4 weeks (group B, n=14), and diabetic group for 12 weeks (group C, n=16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NE-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4周组(14只),C组为糖尿病12周组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后,A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB活性检测。

Methods Turning tests induced by apomorphine and forelimb functional tests were performed. Also, the expression of TH mRNA in mesencephalon of the rats was detected by semi-quantitative analysis of one step RT-PCR.

利用阿朴吗啡诱发旋转试验和前肢功能试验,检测PD大鼠的神经行为学特点,一管法逆转录聚合酶链反应半定量检测其中脑TH mRNA的表达。

Only one complete corollinae chromosome was detected in Ml4, and was determined corollinae chromosome 9. We have used in situ hybridization of genomic DNA to discrimination the parental chromosomes in Beta Ml 4, suppression of cross-hybridization by blocking DNA was not necessary indicating that the investigated Beta genome contain sufficient species-species DNA enabling the unequivocal determination of genomic composition of the hybrids.In our study , the results of BAC -FISH combined with the analysis of GISH in Beta M14,indicating that present of the corollinae chromosome 9 alone was sufficient to confer apospory in Beta M14 upon celluar and molecular..

BAC克隆16-M11和26-L15携带M14花期特异表达的基因;在M14甜菜对应的有性栽培甜菜染色体上没有出现信号,呈半合子状态;包含的单或低拷贝DNA序列在野生白花甜菜进化过程是共线的;两个BAC克隆部分同源;位于末端重组抑制区,所有这些不同寻常的特性都暗示着:BAC克隆16-M11和26-L15和无融合生殖特性相关,可作为无融合生殖

This approach is applied to real images, and the skeletons detected are consistent with visual apperception.

将算法应用于实际图像,检测到了与人视觉感知相一致的目标骨架。

The total genomic DNA of diseased apple leaves was rapidly obtained by modified CTAB method. Through PCR amplification assay, the pathogen of apple scab can be specifically detected from diseased apple leaf tissues and no symptom appearance of apple leaf tissues with pathogen incubation.

采用改进的CTAB法,快速提取发病叶片组织的DNA,并结合PCR检测技术,可从苹果黑星病显症叶片以及未显症而处于潜育期的叶片组织中特异性地检测到苹果黑星病菌。

In the stage of early hypha growth, spore germination, rapid filamentous growth stage and appressorium formation, the STK1 gene expression could be detected.

在产生分生孢子前、产生分生孢子时、菌丝的对数生长期和产生附着胞时都可以检测到STK1基因的表达。

Drug concentration in aqueous humor by injection could be detected in a lower but longer effective time.

观察不同处理组的角膜植片混浊、水肿、新生血管及植片存活时间。

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