查询词典 detected

Methods The expression of ERK1/2, ER and PR in 30 endometrial carcinoma specimens was detected by immunohistochemical method. With the same method, the expression of ERK1/2 was detected in 20 normal endometria specimens and 13 hyperplastic endometria specimens. Semi-quantitative analysis of specimen staining was conducted.

用免疫组化方法检测30例子宫内膜癌石蜡标本中ERK1/2以及ER和PR的表达；同法检测20例正常子宫内膜、13例增生过长子宫内膜石蜡标本中ERK1/2的表达；对标本的染色情况做半定量分析。

In carcinoma cells,Fas was not detected whereas FasL was detected,implicating a mechanism that tumor cells evaded immune attacks.

肝癌细胞中未检出Fas而检出FasL，可能是一种肿瘤逃避免疫攻击的机制。

Methods by using a broad pH range column(Gemini NX C18,4.6mm×150mm,5μm),basic mobile phase which was[H_2O-ammonia-glacial acetic acid] (96:3.6:0.4)-methanol,the related substances in etimicinsulfate were separated and detected by electrospray ionization and positive ion monitoring,the ESI ion source condition was following: temperature is 350℃,nebulizing pressure is 50 psi,dry gas flow is 10 L /min.some related substances in etimicinsulfate were identified by comparing the retention time in chromatography,~+ spectrum and MS~2 spectrum with reference substances\',the others which haven\'t reference substances were identified or speculated by analyzing their MS~2 fragmentation with the help of a rule summarized from the MS~2 fragmentation of gentamicin C1a,micronomicin and etimicin.Results nineteen related substances in etimicinsulfate were separated and detected.

方法利用宽pH范围的Gemini NX C18(4.6mm×150mm，5μm)色谱柱，在碱性条件下，以[水-氨水-冰醋酸(96：3.6：0.4)]-甲醇(70：30)为流动相，直接分离各组分；并用HPLC-ESI-MS~2检测样品中各组分，质谱条件：ESI离子源，离子源温度350□，雾化室压力50 psi，干燥气流速10 L/min，正离子检测方式；对有对照品的有关物质采用比对有关物质与对照品的色谱保留时间、准分子离子的质荷比及二级质谱方法进行鉴定，无对照品的有关物质，则是先通过解析庆大霉素Cla、小诺霉素、依替米星的二级质谱，对该类化合物的二级质谱裂解规律进行归纳，并以此推测未知新化合物进行结构，再用推测的结构对二级质谱进行解析，如所有的碎片离子均合理解释，表明推测合理。

RESULTS: Although PER DNA was detected in the perfused plasma, no productie infectiity was detected.

结果：虽然在灌注过的血浆中检测到PERDNA，但并未检测到明显的感染性。

The quality concentrations of serum E2 were detected by radioimmunoassay. Pituitaries were embedded by paraffin and sectioned continuously. The morphologic figures of pituitary were shown by hematoxylin-eosin stain, the expression of estrogen receptor and progesterone receptor were detected by immunohistochemistry.

术后40 d麻醉后处死动物，放射免疫法检测血清雌二醇质量浓度，垂体组织常规石蜡包埋，连续切片，分别进行苏木精-伊红染色和雌、孕激素受体免疫组织化学染色。

The expression of Fas and FasL in placentas was detected with Western blot and the concentration of soluble Fas and FasL in serum was detected with ELISA method.

Fas/FasL系统在维持机体免疫稳定和免疫赦免中发挥重要作用，是妊娠免疫耐受的重要机制之一[1]，Fas/FasL的异常表达及功能紊乱可能导致病理妊娠的发生。

Result:①out of 25 patients,17 exhibited myeloma-related bone lesions on radionuclide imaging,the positive rate was 68.0%,ribs and spine were involved most frequently,162 lesions showed high uptake of radionuclide,the lesions lied in ribs appeared as strings of pearls,and those in sternums and vertebrae showed linear high uptake,the other 4 showed decreased radionuclide uptake.②the positive rate on radiography was 68.0%(17/25),which was the same as that on radionuclide imaging,the patterns were osteoporosis,pathological fracture,osteolysis or two or two and above of these appearances, radiography detected more abnormalities than radionuclide imaging in identical sites(162 versus 144),but radionuclide imaging detected more lesions than radiography in ribs and sternums.

结果：①25例患者中，骨显像异常17例，阳性率68.0%，肋骨、脊柱最常受累，162处病灶表现为异常放射性聚集，其中，肋骨病灶呈串珠样，胸骨及椎体病变呈扁平状或线状，4处表现为异常放射性减低；②x线片阳性率68.0%（17/25例），与骨显像相同，主要表现为骨质疏松、病理性骨折、骨破坏或呈混合性改变，共同检测的部位中，x线片检出的病灶总数较骨显像多（162和144），但骨显像检出肋骨和胸骨病灶较x线片多。

The positive rate on radiography was 68.0%(17/25),which was the same as that on radionuclide imaging,the patterns were osteoporosis,pathological fracture,osteolysis or two or two and above of these appearances, radiography detected more abnormalities than radionuclide imaging in identical sites(162 versus 144),but radionuclide imaging detected more lesions than radiography in ribs and sternums.

结果：①25例患者中，骨显像异常17例，阳性率68.0%，肋骨、脊柱最常受累，162处病灶表现为异常放射性聚集，其中，肋骨病灶呈串珠样，胸骨及椎体病变呈扁平状或线状，4处表现为异常放射性减低；②X线片阳性率68.0%（17/25例），与骨显像相同，主要表现为骨质疏松、病理性骨折、骨破坏或呈混合性改变，共同检测的部位中，X线片检出的病灶总数较骨显像多（162和144），但骨显像检出肋骨和胸骨病灶较X线片多。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

He became prime minister in 1997 and won international acclaim for his role in the Northern Ireland peace process.

他在1997年成为总理，并且因为在北爱尔兰的和平进程中发挥的作用受到国际社会的赞许。

Mixed Page Sorting Method Using the cuttable user model, this thesis proposes a mixed page sorting method by using cutted user model in server-side and complete user model in client-side.

隐私分级利用的混合式页面排序方法针对个性化搜索中搜索结果排序的问题，本文利用隐私可控开放的用户偏好模型，提出一个在客户端和服务器端分步进行的隐私分级利用的混合式页面排序方法。