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The results show that the duration of the 3 phases during the culture of cell suspension is related to the inoculum size, the initial nutrient concentration, the feeding of nutrients and the culture conditions such as temperature and dissolved oxygen, that the maximum cell yield reaches about 12g/L for batch culture or 35g/L for fed-batch culture in dry weight, that the saponin content of cells quickly increases in the bloom phase and keeps on increasing until the early stage of the declining phase, with a maximum saponin content of about 60~70g/kg in dry weight, that the conductance value of cell suspension decreases from more than 8000μS to less than 600μS with the growth of cells, but increases when the cells autolyze, and that part of cells turns red in the later culture, which has no apparent effect on the saponin content.

结果显示:细胞3个生长时期的持续时间与接种量、初始营养浓度、营养流加情况及培养条件有关;分批培养和流加培养时的最大细胞得率分别为12g/L和35g/L;细胞的皂甙含量在旺盛生长期快速上升,并保持增长至衰退期前期;干细胞中最大的皂甙含量达60~70g/kg;随着细胞的生长,培养液的电导值从8000μS以上下降至600μS以下,细胞自溶后逐渐回升。另外,培养后期部分细胞颜色变红,但没有发现颜色转变对皂甙含量造成影响。

Possible involvement of syntaxin4 and SNAP-23 in exocytosis from human natural killer cells to Porcine Endothelial Cells It was reported that the SNAREs protein complex are involved in many human immune cells and participated in exocytosis of the granules, such as platelet, macrophages and basophilic cells.

SNARE是一种参与神经递质释放的胞内蛋白质,在介导分泌性囊泡运动和融合中发挥重要作用,也参与某些免疫相关细胞的囊泡运动、效应物质分泌和免疫效应调节等。

The digestive gland is a compound gland in which the tubules consists of two types of cells: digestive cells and basophilic cells. The digestive cells contain many vesicles and granules.

消化腺为复管状腺,腺管上皮有消化细胞和嗜碱性细胞两种类型,消化细胞内含许多囊泡和颗粒。

At 4th week, the percentages of Clara cells over bronchiolar epithelial cells were similar between groups. At 24th week, the percentages of Clara cells were reduced in mice exposed to TCDD, NNK, CSC, or TCDD+CSC exposure, but not in mice exposed to TCDD+1 mg NNK. Thus, these findings suggested that TCDD, NNK, CSC, or TCDD+CSC exposure injuried Clara cells to lose CCSP protein in mice lung.

在A/J mice 4周实验组中,TCDD或NNK各实验组Clara cell的数目(26.6%-34.3%)与控制组(34.2%)比较,并没有明显的差异;反观A/J mice 24周实验组中,除了TCDD+NNK (1 mg)实验组外,其他各实验组Clara cell的数目与控制组(55.9%)比较明显下降11.7%-41.4%,显示暴露NNK、TCDD、CSC或TCDD+CSC皆会伤害Clara cell,暗示Clara cell对这些环境毒物比其他呼吸道上皮细胞敏感。

We did bronchovesicular lavage with 10 mL normal saline, then collected all cells in BALF, stained cells by Giemsa's stain and counted the total number of cells and differential cells numbers for observing the inflammatory changes in lungs after RSV infection.

以10 ml生理盐水进行支气管肺泡灌洗,收集肺泡灌洗液中细胞,常规吉姆萨染色,行细胞分类计数,观察RSV感染对肺脏的炎症影响。

This paper reviews the pathways of ES cells in vitro being committed and differentiated to hematopoietic cells, cardiomyocytes, neural cells, adipocytes, pancreatic islets, endothelial cells, epithelia, osteoblasts, chondroblast and melanocytes.

文章综述了体外定向诱导ES细胞分化为造血细胞、心肌细胞、神经细胞、脂肪细胞、胰岛细胞、内皮细胞、上皮细胞、成骨细胞、软骨细胞和黑素细胞等的途径和方法。

After the cells were treated by high concentration toxin for 4d, the content of nitrate-nitrogen in cells were lower markedly than the contrast, after the cells were treated by middle and low concentration toxin for 7d, the content of nitrate-nitrogen in cells were lowered. 3 groups of toxin treating all lowered the total phosphorus content. At the end of test, the phosphorus contently of low, middle, high concentration treating groups were 74.98%, 76.47% and 84.00% of the contrast respectively. The ACP activity of 3 treating groups compared with the contrast appeared the tendency of first lowering then raising.

高浓度毒素处理细胞4d后,细胞内硝态氮含量显著低于对照;中、低浓度毒素处理细胞7d后降低了胞内硝态氮含量。3组毒素处理均降低了胞内总磷含量,到实验结束时,低、中、高浓度处理组的胞内磷含量分别为对照的74.98%、76.47%和84.00%。3组处理组ACP活力与对照相比呈现先降低后升高的趋势。

Furthermore, we also observed a kind of medium type cells intermediate between squamous and cuboidal cells, whose cytoplasm and nucleus were much better developed than those of squamous cells but less well developed than those of cuboidal cells.

另外,我们还观察到一种介于扁平形细胞和立方形细胞之间的中间型细胞,其胞质和核的发育明显好于扁平形细胞,但比立方形细胞差。

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果:在绒毛组织中,IL-8蛋白定位于绒毛上皮细胞的细胞质内,且病例组的表达明显高于对照组;在蜕膜组织中;病例组蜕膜细胞的胞质内可见IL-8蛋白表达,且高于对照组;H-E染色可见病例组绒毛组织的滋养层变薄,细胞变性甚至坏死、嗜酸性增强,绒毛中轴纤维化程度增强;蜕膜组织中蜕膜细胞失去细胞间连接,部分蜕膜细胞解体、核消失。

Epidermal cells near the wound dedifferentiate and proliferate forming blastema and/or migrate to the wound plane forming a pre-epidermis consisting of several layers of stem cells covering the wound plane, which then re-differentiates further forming fully developed epidermis. Cells in the parietal peritoneum are also induced by injury to dedifferentiate into stem cells which then proliferate and migrate along the parietal peritoneum to the wound place forming a pre-peritoneum which re-differentiates into fully developed parietal peritoneum.

伤口愈合包括伤口闭合、顶端表皮层及体腔上皮的新生:创伤后的前4 d,残腕顶端的肌肉组织向伤口处迁移并重排使伤口闭合;创伤附近的表皮层细胞发生脱分化并增殖后迁移到创伤面形成由数层干细胞组成的前表皮层,之后进一步分化形成表皮层;而体腔上皮细胞在创伤诱导下也脱分化并增殖,然后沿体腔上皮迁移到创伤处形成&前体腔上皮&,经再分化形成新的体腔上皮。

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