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Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；底板；神经管；轴索；神经胚形成；成神经细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；gangliogenesis；胚胎发生；axonogenesis；retinogenesis；gliogenesis；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管故障；无脑；脊柱裂；成神经细胞瘤细胞；细胞迁移；神经发生；发展；发展进程首页上一页下一页末页共有 22 条记录，本页从第 11 到第 20 条。

The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.

本研究分别对胎鼠听囊、新生鼠听觉上皮和成年鼠听觉上皮用自然培养方法进行体外培养，并应用免疫细胞化学方法，对培养细胞进行染色标记和观察，系统了解哺乳动物听觉细胞发生、发育过程，以及这些细胞在体外的增殖和分化过程，旨在了解哺乳动物听觉细胞听觉上皮感觉细胞的来源，发现细胞生长的前体细胞。

We discovered that the mechanisms of borneol opening BBB involved (1) promoting the tight junction of endothelial cells opening:(2) increasing the quantity of pinocytosis vesicle in endothelial cells and enhance the pinocytosis;(3) inhibiting the activation of P-glycoprotein (P-glycoprotein is an efflux pump of drugs) and increase the permeability of BBB;(4) reducing the expression of intercellular adhesion molecule-1 in brain microvessel endothelial cells;(5) increasing the concentration of Ca〓 in brain microvessel endothelial cells;(6) increasing the activation of eNOS in brain microvessel endothelial cells.

随后，在对冰片开放血脑屏障的机制作进一步的研究时又发现，冰片开放血脑屏障的机制包括以下几个方面：(1)冰片可使血脑屏障内皮细胞间的紧密连接开放；(2)冰片能使内皮细胞内的囊泡数量增加，吞饮功能增强；(3)冰片能抑制P-糖蛋白的活性（P-糖蛋白是一种药物外排泵），而使血脑屏障的通透性增加；(4)冰片能使脑微血管内皮细胞ICAM-1表达量减少；(5)冰片使脑微血管内皮细胞内的Ca〓浓度升高；(6)冰片可升高脑微血管内皮细胞eNOS的活性。

RESULTS: Both resting B cells and T cells did not express PD-L1 on their surface, however PD-L1 expression was significantly up-regulated on the surface of the activated B cells after 6 h stimulation with LPS or pokeweed mitogen, and the percentages of B cells that expressed PD-L1 reached a plateau at 24 h, which were (46.26±10.71)% with LPS and (43.67±6.14)% with PWM stimulation, respectively. No markedly change of PD-L1 expression on the surface of the activated T cells after stimulation with LPS was observed, but upregulation of PD-L1 expression was observed when stimulation with PWM.

结果： 静息B细胞和T细胞表面不表达PD-L1，LPS和PWM刺激6 h后表达PD-L1的B细胞百分率均显著高于静息B细胞，24 h达到最高，分别为(46.26±10.71)%和(43.67±6.14)%，之后随时间延长而降低；表达PD-L1的T细胞百分率在LPS刺激下无显著变化，但PWM刺激6 h后百分率明显高于静息条件，24 h达到最高，为(25.42±9.23)%，之后下降。

After 24 hours of incubation,the cells became bigger,irregular and typical DCs with pseudopodium. The floating cells were separately induced to CIK cells and LAK cells. CIK cells proliferated rapidly and became a lot of large growth-colonies.

悬浮的细胞分别用于诱导生成CIK和LAK细胞，3天后CIK细胞生长迅速，出现大量的生长集落，体积明显变大呈多形性。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Results The hela cells which transfected with HSV-TK and IL-12 gene was obviously changed in appearance after 24 hours which added GCV, but the normal control cells growthed prosperity. The survival rate of the hela cells which transfected with HSV-TK and IL-12 gene was fall-off with the plus of tile GCV. The killing effect of GCV for the hela cells transfected with HSV-TK and IL-12 gene was more strengthener than that for the normal cells.

结果 加入GCV后体外培养联合基因转染的Hela细胞，24h后细胞明显发生形态上改变，正常对照组细胞生长旺盛；随着GCV剂量的加大，转染联合基因的Hela细胞的存活率呈逐渐下降的趋势，GCV对转染细胞的杀伤作用较对正常细胞的杀伤作用明显增强。

METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基－N'-琥珀酰基壳聚糖进行标记，再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养，通过流式细胞仪及酶联免疫检测仪测定N-正辛基－N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

The properties of TCC with ATS were as good as the TCC with HTS, better that the requirement of standard GB8744-88 for semi-graphitic carbon block. As good as TCC with HTS in industrial cells, the TCC with ATS also reduces the sodium penetration to cathodic lining, no tear-off of the coating was found after starting up of the cells. As compared with reference cells, the operation of coating cells with ATS was also improved to keep good shape of cell chamber, reduce the formation of sludge on the cell bottom, and gain more uniform current distribution. The ohmic drop of TCC cell bottom was lessened by 11. 5mV. The current efficiency was increased by about 1. 48% in average. It was estimated that the TCC life could be 44 months. Obviously, the TCC with ATS could also improve the performance of industrial cells, save energy, and increase the cell life.

对常温固化涂层槽的考核表明，与高温固化涂层槽一样，阴极内衬对钠的吸收减少，钠渗透速度减缓，从而减小钠膨胀危害，减少了铝电解槽早期破损的几率；启动后，常温固化涂层不脱落，同样具有良好的粘接性能；与对比槽相比较，常温固化涂层槽正常生产后，阴极表面干净、光洁，无沉淀或结壳，炉膛规整，阴极、阳极电流分布均匀，炉底压降降低（平均降低11.5mV），电流效率提高（平均提高1.48％）；涂层在电解槽内存在的时间可达到44个月以上；常温固化涂层同样可以起到改善电解槽工作状态、节能降耗、延长槽寿命的作用。

Lymphoid stem cells can produce a few, but not all, kinds of blood cells. Finally, cells that only produce identical copies of themselves are called unipotent. Like skin cells, when they divide, they only make more skin cells.

淋巴干细胞可不像造血干细胞那样能造出所有血液类型的细胞，它们只能创造复制出自身相同的细胞，比如皮肤细胞，当它们分裂的时候，只能够制造皮肤细胞，我们把这些干细胞叫单一干细胞

The small blue capacitor has very high chances to go fully shorted compare to electrolytic capacitor which is very rare.

小蓝电容器具有很高的机会去充分地缩短了比较电解电容器，这是非常罕见的。

In the Italian campaigns of 1513 and 1515, when the Swiss won the victories of Novara and Marignan, he acted as army chaplain.

在意大利的运动， 1513年和1515年，当瑞士赢得了胜利，诺瓦拉和marignan ，他担任军队的牧师。