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The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物,且所有试材的扩增结果一致,均无目的条带以外的产物扩增,说明Ty1-copia类逆转座子在苹果属植物中广泛存在,且存在历史可能较为久远;从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066),结果表明,同一基因组来源的这些序列存在高度的异质性。

The hypothesis on the equivalence in non conservative system is set up for irreversible process on the basis of bring\|in of the nonconservative noninertial system;and the hypothesis on the covariance in non conservative system is put forth for the space time with complex action on the basis of bring\|in of the ultra gauge.

在引入非保守非惯性系的基础上对不可逆过程建立非保守系等效性假设,在引入广域度规的基础上对具有复杂行为的时空建立非保守系协变性假设;利用密度分布的不均匀度h和粗粒熵S及推导的多标度因数η计算式,引入非保守惯性质量和非保守引力质量。

Methods: Clinical data of 145 EP patients were retrospectively reviewed; of them, medical treatment, conservative operation and conservative operation plus oviductal injection of 10 mg of methotrexate occurred in 62, 38 and 45 cases, respectively.

回顾性分析保守性治疗输卵管妊娠145例,其中药物治疗组62例,单纯保守性手术38例,保守性手术加术中输卵管局部注射甲氨蝶呤10mg 45例。

On the base of reviewing the development of NSP models, some deficiencies of existent models are analysed and listed as follows: most models can be applied only to very small watershed; mechanism models involve too many parameters which can not be calibrated properly by limited data; amoung practical models, empirical models are much more common than mechanism models; most models are focus on the conservative soluble pollutants rather than non-conservative and particulate pollutants; almost all models rely highly on the measured data, and there are fewer studies about the load estimation for ungauged areas.

本文在评述非点源污染模型研究进展的基础上,分析了现有模型的不足,主要有:大多数模型只能适应于很小的流域面积;机理类模型参数过多,率定困难,应用效果不理想;实用模型中,经验类模型多,机理类模型少;对保守性的液态污染物研究得较多,对非保守性和固态污染物研究得较少;各类模型均高度依赖于实测资料,无资料地区的模型或预测方法研究得很少。

The more conservative your field is, the more conservative your suit should be.

并且你的领域越是保守,你的着装也要相应的越保守。

The other 7 cases were cured by transferring to operation that was carried out in 4 cases because of strangulated intestinal obstruction during conservative treatment and in 3 because of invalid conservative treatment for 40 days.

回顾性分析我院1996年1月至2006年1月收治的61例术后早期炎性肠梗阻患者的临床资料。结果 54例患者经保守治疗痊愈,平均住院时间10.2 d.7例手术治疗后痊愈,其中4例因保守治疗时出现绞窄性肠梗阻而手术,3例保守治疗40 d无效而手术。

After the local party chose a moderate to run in the special election there, prominent conservative Republicans, led by Sarah Palin, took it upon themselves to campaign not for their own party but for the candidate of the tiny Conservative Party of New York State.

在当地的共和党选出了一位候选人后,莎拉·佩林领导的保守派共和党人自作主张的为纽约保守党的候选人而不是他们自己的候选人竞选助阵。

Dan Wu city is in however reforming and opening in 20 old time, conservative and complacent and conservative, backward, miss the good opportunity of development for many times, its economy actual strength and political influence are in Guangxi suffer a disastrous decline, be in the puniest position in city of 4 areas administer, economy grows long-term lag.

但梧州却在改革开放20多年的时间里,故步自封、保守落后,多次错失发展的良机,其经济实力和政治影响在广西一落千丈,处于四个区辖市中最弱小的地位,经济发展长期滞后了。

I cite a case, price of this word contest is as high as tumor 16 yuan are clicked every time, one day clicks the word of 1000 is then 16 thousand yuan everyday, lead the 1% words that will calculate by changeover (conservative estimation is inside this limits), so a day of 1000IP the word has 10 people is duped, 16 thousand yuan of affirmation of a day want these 10 people to come namely burden, so be equivalent to you treat disease most most the injustice money that the most conservative affirmation should give is the least 1600 yuan (affirmative of course and more than this, the person that just says to see a doctor here also should do a good turn cost of price giving contest, sweat).

我举个例子,肿瘤这个词竞价高达16元每次点击,一天点击1000的话那就是1.6万元每天,按转换率1%来算的话,那么一天1000IP个话有10个人是上当的,一天的1.6万元肯定就是要这10个人来负担,那么相当于你去看一次病最最最保守肯定要出的冤枉钱最少是1600元(当然肯定不止这个,我这里只是说看病的人也要帮忙出竞价费,汗)。

But as a proportion of the shrinking religious population, fundamentalist Protestantism, conservative Catholicism and orthodox Judaism have been expanding, while mainline Protestantism, liberal Catholicism and reform and conservative Judaism are losing adherents, mostly to non-belief.

但当宗教人口急速萎缩,基本教义派的新教{、传统的天主教徒及传统的回教徒正在扩张而主要的新教徒、自由的天主教徒及改革者、保守的犹太教徒正在失去信徒,大部份变成无信仰者。

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Certain types of sockets are ''connectionless'', most commonly

某些类型的套接字是无连接的,大多数是UDP协议。

Take it in to his nibs yourself .

你自己拿进去给头儿吧。

HoMedics has been around for just over 20 years, and yet in that time they have become recognized as a leader in the health and wellness industry.

HoMedics已经有超过20年,但在那个时候,他们已经成为公认的领导者,在卫生和健康产业。