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In this study, isolate 23 microbes with lipase activity from Wu-Jie scrap heap at Ilan and Taichung Environmental Protection Section. When the microbes incubator in 50oC, there are isolates B61 and M63 that lipase activity are better than others. Brevibacillus borstelensis SH168 isolated from NTU resterant food waste compost is used as control check. Isolates B61, M63 and Brevibacillus borstelensis SH168 determine the lipase activity by the LB with tributyrin. The lipase activity of isolates B61 and M63 are 2~3 fold higher than Brevibacillus borstelensis SH168. So isolates B61 and M63 are more useful in producing lipase. Isolate B61 can grow at pH 5~9, and at temperature 20oC ~ 60oC; isolate M63 can grow at pH 6~10, and at temperature 30oC ~ 50oC. Isolates B61 and M63 were identified by 16S rRNA. Isolate B61 is Bacillus circulans, and isolate M63 is Bacillus species.

本研究,於宜兰五结垃圾场和台中市环保局厨余堆肥样品中共分离出 23 株具脂肪酶活性菌株, 23 株菌株中经过三油酸甘油酯的洋菜培养基於 50℃培养 5 天后,发现有 2 株菌的脂肪酶活性较强,分别为分离株 B61 和 M63;以本实验室先前自台大女九餐厅蔬果厨余堆肥中所筛选出的耐高温脂解菌 Brevibacillus borstelensis SH168作为本实验对照菌株,将分离株 B61、M63 及 Brevibacillus borstelensis SH168 3 株菌株於含有三酪酸甘油酯液态培养基培养并测定其脂肪酶活性,发现分离菌株之活性皆比 Brevibacillus borstelensis SH168 高出 2~3 倍,认为分离株 B61、M63 适於产生脂肪酶的菌株,分离株 B61 在 pH 5~9 皆可生长,生长温度介於 20 oC ~ 60oC;分离株 M63 在 pH 6~10 可生长,生长温度介於 30 oC ~ 50oC; 2 株分离菌株以 16S rRNA定序结果分离株 B61 为 Bacillus circulans,分离株 M63 为 Bacillus 属。

At vacuous status, the predominant mieroflora in the intestinal tract was E. coli, then was anaerobic at un-ingestive stage and tubificidae fed stage, the third was bacillus, at artifIcial formulated feed stage, the second was bacillus, and enterococcus was emerged.

结果表明:在空腹状态下肠道菌群组成以大肠菌群为主,未开口期和水蚯蚓投喂期厌氧菌第二,其次为芽孢杆菌;投喂人工配合饲料后芽孢杆菌第二;投喂人工配合饲料后出现肠球菌。

Resistance of Bacillus subtilis var. niger Spores Occluded in Water-insoluble Crystals to Three Sterilization AgentsJOHN E. DOYLE AND ROBERT R. ERNSTResearch Laboratory, Castle Company, Rochester, New York 14623The resistance to destruction of spores of Bacillus subtilis var. niger occluded in crystals of calcium carbonate and exposed to ethylene oxide and moist and dry heat was determined and compared with the destruction of unoccluded spores.

检测了封闭在碳酸钙晶体中的枯草杆菌黑色变种芽孢暴露于环氧乙烷、湿热和干热中的耐受性,并与未封闭孢子的破坏相比。

A screening and cultivation method for Bacillus thuringiensis of high-yield high temperature resisting protease includes the following steps: high-yield high temperature resisting protease Bacillus thuringiensis Bt140 is obtained through adopting casein substrate agar to identify flat primary screen and fermenting enzyme activity measuring secondary screening; Plackett-Burman design and response surface analytical method are adopted to obtain optimal fermentation zymogenic formula and fermentation condition for Bt140 quickly; finally, shaking fermentation level reaches to 918.56U/mL.

一种高产耐高温蛋白酶的苏云金芽孢杆菌筛选及培养方法,采用酪蛋白底物琼脂鉴定平板初筛与发酵酶活测定复筛的方法获得高产耐高温蛋白酶苏云金芽孢杆菌Bt140;采用Plackett-Burman设计与响应面分析法快速获得了Bt140最佳的发酵产酶配方与发酵条件,最终摇瓶发酵水平可达918.56U/mL。

The main results are as follows:(1)In order to find out the catalysis character of pectinase and xylanse in bacillus circularise A_6 and exhibit their maxium catalysis rate,the optimum condition for determination of pectinase and xylanase activity in bacillus circularise A_6 was studied in aspect of pH,temperature,substance concentration,reaction time,anion concentration.

试验结果如下:(1)为探明环状芽孢杆菌果胶酶及木聚糖酶的催化特性,更好发挥它们的催化性能,本文从pH值、温度、底物浓度、反应时间、离子强度几个方面研究了环状芽孢杆菌A_6产生的果胶酶与小聚糖酶活性测定的最适方法。

The strain BEB2 of endophytic Bacillus isolated from banana was identified as Bacillus subtilis according to its homology analysis of 16S rDNA sequence. The temperature, pH and medium had a significant impact to the inhibition of BEB2 to the banana wilt disease.

利用聚合酶链式反应方法对细菌BEB2做进一步的分子鉴定,并研究不同温度、pH值和培养基下BEB2发酵液对香蕉枯萎病菌生长的抑制能力,以及应用BEB2对香蕉枯萎病进行防治测试。

Results In 136 patients, a total of 72cases sputum culture positive, the positive rate of 52.9% sputum culture for three consecutive single bacteria were 16 cases, the positive rate of 11.8%.16 patients sputum results were:hemolytic streptococcus 6 cases, 4cases of Pseudomonas aeruginosa , Klebsiella pneumoniae in 2 cases and gas production Bacillus 2 cases , Enterobacter Bacillus 2 cases, 136 patients with 386 cases sputum culture times , 106 times positive(27.5%), and consecutive three or more times a train 4 the same bacteria ,only 42 cases(10.8%); the same single-64 cases sputum culture , which amounts to 16.6%.

结果 在136例患者,共72例痰培养阳性,阳性率52.9%,痰培养连续3次为单一细菌者为16例,阳性率为11.8%。16例患者痰菌结果分别为:溶血性链球菌6例,绿脓杆菌4例,克雷伯氏杆菌2例,产气杆菌2例,阴沟杆菌2例,136例患者386例次痰菌培养,106例次阳性(27.5%),而连续3次以上培养为相同一细菌,仅为42例次(10.8%);非相同单一痰菌培养64例次,占16.6%。

Bacillus subtilis CCRC 14199 uricase is similar (61% amino acid identity) to the uricase from Bacillus sp.

TB-90之尿酸氧化酵素的氨基酸序列发现相似度为61%。

One strain Bacillus badius was identified from oil-containing wastewater. Our studies provide a basis for investigating the role of Bacillus badius in oil degradation and its mechanisms as well.

从含油脂废水中筛选出1株栗褐芽胞杆菌,为下一步实验证实其分解油脂的作用和机制奠定了基础。

Results Colifrom bacterium,staphylococcus albus,cerea bacillus,bacillus aeruginosus and yeast fungus can resist to arsenite.

结果 绿脓杆菌可耐受100mg/L的As3+浓度,大肠埃希菌、白色葡萄球菌、蜡样芽孢杆菌和酵母菌均可耐受1 000mg/L的As3+浓度;大肠埃希菌对As3+有一定的富集能力。

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