查询词典 chimeric

Is the chimeric protein of human epidermal growth factor and PE40 which is recombinant protein of the translocated subunit and killing subunit of Pseudomonas exotoxin A.

EGF-PE4 0是由人表皮生长因子基因与绿脓杆菌外毒素 A的跨膜亚单位和毒力亚单位( PE4 0 )基因融合，在大肠杆菌内表达的重组毒素。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

The leafstalk, chief vein, bud, the cane top on side branch, bast and xylem of type B had type A and C tow components, belonging to chimeric tissues. The leaf pulp and thin leaf vein had no components of type C, belonging to pure tissues of type A. Type B plant had two kinds of color roots, the rufous roots belonged to type A tissue, the yellow roots belonged to type C tissue through the SSR identification.

深州杨B型的叶柄、叶主脉、芽、侧枝茎尖、韧皮部、木质部同时检测到深州杨A、C型两种成分存在，属于嵌合组织，B型叶肉及细叶脉中检测不到深州杨C型成分，属于深州杨A型纯合组织；深州杨B型植株的地下部分含有红褐色和土黄色两种根，经检测，红褐色根是属于深州杨A型的纯合组织，土黄色根是属于深州杨C型的纯合组织。

After immunization, kinetics of antibody response, isotype of IgG antibody, and antibody responses to the individual components of the chimeric antigen were detected by ELISA. Interactions of the antibodies with native antigens located on surface of merozoite of Plasmodium falciparum were analyzed by indirect immunofluorescent assay.

以ELISA检测免疫血清中特异性抗体的动态变化、IgG抗体亚型及对融合抗原各组分的抗体水平，以间接荧光抗体实验分析免疫血清对恶性疟原虫天然抗原的识别情况。

By adding a sequence encoding a small peptide,〓, to the base sequence of pYZFcd20, a new phasmid pYZcpp3 was constructed. Using the pYZcpp3 to transform E. coli. 16c9, the genetically engineered bacteria 10916# was obtained. 10916# can secret the soluble chimeric antibody fragment anti-CD20 F〓 into periplasmic.

通过对质粒pYZFcd20的碱基序列的改造，在CH1基因的末端添加了一截二聚化结构的编码序列，实现了在大肠杆菌中直接表达可溶性抗CD20嵌和抗体片段F〓的目的。

The results indicated that:The multi\|locus DNA fingerprint with a new synthesized probe\|JL\|02,has enough polymerism and good stability,and should be very useful to monitor the chimerism in different tissues of ES cell chimeric mouse and to check whether an ES cell line has the capacity to enter the germ line,especially when involving strains that can not be discerned with coat color or biochemical markers.

结果表明：采用新型的人工合成的寡核苷酸多聚体探针(JL-02探针)的多位点DNA指纹图谱，具有足够的多态性和很好的稳定性。适用于鉴定ES细胞在嵌合体小鼠各种组织器官中的嵌合情况，也适用于鉴定ES细胞是否具有种系嵌合能力，尤其适用于在嵌合体研究中，最适宜的品种组合具有相同的毛色或相同的酶型。

Methods: Chimeric Ab production in culture supernatant was measured by ELISA.

用ELISA方法检测转染瘤细胞培养上清的抗体分泌量。

Chimeric proteins were expressed in COS-7 and CHO cell culture supernatant and confirmed by ELISA and Western blot.

ELISA 和Western blot确定在COS-7和CHO细胞中表达了CD40-Ig融合蛋白。

In 8 cases, both the results were consistent excepting for minor discrepancies.③In 3 cases, including 2 each with triploidy and tetraploidy respectively, and one with chimeric karyotype of normal/+22, the results from the two methods were discrepant.

CGH与常规R带的分析结果比较可分为三种：①CGH和常规核型分析结果完全一致的有3例；②CGH分析和R带分析从不同角度提供了相对一致信息的有8例；③CGH分析和R带分析结果不一致的有3例，其中2例分别为约三倍体和约四倍体，另1例为正常/＋22嵌合型。

Methods The HPV16E7 gene was cut to three parts, amplified by PCR, and connected individually with BPVL1 on plasmid PUC. With PVL1393 as a transfect vector, the BPVL1/HPV16E7 recombinants were transfected to baculovirus which subsequently expressed the chimeric BPVL1/HPV16E7 protein in the SF－9 cells. The recombinant proteins were then purified by centrifuge, sonication, sucrose and CsCl ultracentrifuge, and were identified by SDS-PAGE, Western blot, ECL and TEM.

HPV16E7基因分3段经PCR扩增后分别克隆入连有BPVL1的质粒PUC形成BPVL1-HPV16E7；以质粒PVL1393为载体将BPVL1-HPV16E7基因转染杆状病毒并在昆虫细胞中进行表达；用超声粉碎和蔗糖超离、氯化铯梯度离心等方法纯化以及免疫印迹、ECL、透射电镜等方法鉴定表达产物。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。